The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications
To reveal mtgenome characterizations and reconstruct phylogenetic relationships of Hylicinae, the complete mtgenomes of four hylicine species, including <i>Nacolus tuberculatus</i>, <i>Hylica paradoxa</i>, <i>Balala fujiana,</i> and <i>Kalasha nativa</i&g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/12/869 |
| _version_ | 1797545504881508352 |
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| author | Jiu Tang Weijian Huang Yalin Zhang |
| author_facet | Jiu Tang Weijian Huang Yalin Zhang |
| author_sort | Jiu Tang |
| collection | DOAJ |
| description | To reveal mtgenome characterizations and reconstruct phylogenetic relationships of Hylicinae, the complete mtgenomes of four hylicine species, including <i>Nacolus tuberculatus</i>, <i>Hylica paradoxa</i>, <i>Balala fujiana,</i> and <i>Kalasha nativa</i>, were sequenced and comparatively analyzed for the first time. We also carried out the richest (11) subfamily sampling of Cicadellidae to date, and reconstructed phylogenetic relationships of Membracoidea among 61 species based on three datasets using maximum likelihood and Bayesian inference analyses. All new sequenced mtgenomes are molecules ranging from 14,918 to 16,221 bp in length and are double stranded, circular in shape. The gene composition and arrangement of these mtgenomes are consistent with members of Membracoidea. Among 13 protein-coding genes, most show typical ATN start codons and TAR (TAA/TAG) or an incomplete stop codon T–, and several genes start by TTG/GTG. Results of the analysis for sliding window, nucleotide diversity, and nonsynonymous substitution/synonymous substitution indicate <i>cox1</i> is a comparatively slower-evolving gene while <i>atp8</i> is the fastest gene. In line with previous researches, phylogenetic results indicate that treehopper families are paraphyletic with respect to family Cicadellidae and also support the monophyly of all involved subfamilies including Hylicinae. Relationships among the four hylicine genera were recovered as (<i>Hylica</i> + (<i>Nacolus</i> + (<i>Balala</i> + <i>Kalasha</i>))). |
| first_indexed | 2024-03-10T14:16:24Z |
| format | Article |
| id | doaj.art-3f7f3613be9c4b9fa03a1285ab4156ff |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T14:16:24Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-3f7f3613be9c4b9fa03a1285ab4156ff2023-11-20T23:45:13ZengMDPI AGInsects2075-44502020-12-01111286910.3390/insects11120869The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic ImplicationsJiu Tang0Weijian Huang1Yalin Zhang2Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaTo reveal mtgenome characterizations and reconstruct phylogenetic relationships of Hylicinae, the complete mtgenomes of four hylicine species, including <i>Nacolus tuberculatus</i>, <i>Hylica paradoxa</i>, <i>Balala fujiana,</i> and <i>Kalasha nativa</i>, were sequenced and comparatively analyzed for the first time. We also carried out the richest (11) subfamily sampling of Cicadellidae to date, and reconstructed phylogenetic relationships of Membracoidea among 61 species based on three datasets using maximum likelihood and Bayesian inference analyses. All new sequenced mtgenomes are molecules ranging from 14,918 to 16,221 bp in length and are double stranded, circular in shape. The gene composition and arrangement of these mtgenomes are consistent with members of Membracoidea. Among 13 protein-coding genes, most show typical ATN start codons and TAR (TAA/TAG) or an incomplete stop codon T–, and several genes start by TTG/GTG. Results of the analysis for sliding window, nucleotide diversity, and nonsynonymous substitution/synonymous substitution indicate <i>cox1</i> is a comparatively slower-evolving gene while <i>atp8</i> is the fastest gene. In line with previous researches, phylogenetic results indicate that treehopper families are paraphyletic with respect to family Cicadellidae and also support the monophyly of all involved subfamilies including Hylicinae. Relationships among the four hylicine genera were recovered as (<i>Hylica</i> + (<i>Nacolus</i> + (<i>Balala</i> + <i>Kalasha</i>))).https://www.mdpi.com/2075-4450/11/12/869mitochondrial DNAleafhopperHylicinaephylogeny |
| spellingShingle | Jiu Tang Weijian Huang Yalin Zhang The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications Insects mitochondrial DNA leafhopper Hylicinae phylogeny |
| title | The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications |
| title_full | The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications |
| title_fullStr | The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications |
| title_full_unstemmed | The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications |
| title_short | The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications |
| title_sort | complete mitochondrial genome of four hylicinae hemiptera cicadellidae structural features and phylogenetic implications |
| topic | mitochondrial DNA leafhopper Hylicinae phylogeny |
| url | https://www.mdpi.com/2075-4450/11/12/869 |
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