Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods
Andrias davidianus (Chinese giant salamander, CGS) is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China. However, the progress of functional peptides mining was slow due to lack of reference genome and protein sequence data. In this...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001143 |
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| author | Changge Guan Zhenglin Tan Shucheng Li Yi Wang Naoyuki Yamamoto Chong Zhang Songjun Wang Junjie Chen Xin-Hui Xing |
| author_facet | Changge Guan Zhenglin Tan Shucheng Li Yi Wang Naoyuki Yamamoto Chong Zhang Songjun Wang Junjie Chen Xin-Hui Xing |
| author_sort | Changge Guan |
| collection | DOAJ |
| description | Andrias davidianus (Chinese giant salamander, CGS) is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China. However, the progress of functional peptides mining was slow due to lack of reference genome and protein sequence data. In this study, we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA sequences. By functional annotation and amino acid composition analysis, we have discovered various genes related to signal transduction, and 16 genes related to longevity. We have also found vast variety of functional peptides through protein coding sequence (CDS) analysis by comparing the data obtained with the functional peptide database. Val-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis, suggesting that our approach is reliable. This study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat, thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences. |
| first_indexed | 2024-03-12T03:52:20Z |
| format | Article |
| id | doaj.art-3f81933d97aa44cab7b41792fb263b2d |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T03:52:20Z |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-3f81933d97aa44cab7b41792fb263b2d2023-09-03T12:14:16ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131166172Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foodsChangge Guan0Zhenglin Tan1Shucheng Li2Yi Wang3Naoyuki Yamamoto4Chong Zhang5Songjun Wang6Junjie Chen7Xin-Hui Xing8Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Corresponding authors.School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-0026, Japan; Sensing System Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, 305-8565, JapanKey Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-0026, JapanKey Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing 100084, ChinaSchool of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-0026, JapanKey Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaHenan Giant Salamander Protection and Development Association, Luoyang 471000, ChinaIntelligent Computing Research Center, Harbin Institute of Technology, Shenzhen 518071, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China; Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 518067, China; Corresponding authors.Andrias davidianus (Chinese giant salamander, CGS) is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China. However, the progress of functional peptides mining was slow due to lack of reference genome and protein sequence data. In this study, we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA sequences. By functional annotation and amino acid composition analysis, we have discovered various genes related to signal transduction, and 16 genes related to longevity. We have also found vast variety of functional peptides through protein coding sequence (CDS) analysis by comparing the data obtained with the functional peptide database. Val-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis, suggesting that our approach is reliable. This study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat, thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences.http://www.sciencedirect.com/science/article/pii/S2213453023001143Chinese giant salamanderTranscriptomic analysisBioactive componentsFunctional peptides mining |
| spellingShingle | Changge Guan Zhenglin Tan Shucheng Li Yi Wang Naoyuki Yamamoto Chong Zhang Songjun Wang Junjie Chen Xin-Hui Xing Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods Food Science and Human Wellness Chinese giant salamander Transcriptomic analysis Bioactive components Functional peptides mining |
| title | Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods |
| title_full | Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods |
| title_fullStr | Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods |
| title_full_unstemmed | Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods |
| title_short | Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods |
| title_sort | transcriptomic analysis of andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods |
| topic | Chinese giant salamander Transcriptomic analysis Bioactive components Functional peptides mining |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001143 |
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