Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L.
Abstract Thirteen fungi that produce compounds with herbicidal activities were isolated, identified, and extracted under the assumption that the mechanism of action occurs during seed exposure to the extract. The extracts from all the fungal strains considerably decreased the growth parameters of Am...
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46201-0 |
| _version_ | 1797636884853161984 |
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| author | Nutcha Manichart Chamroon Laosinwattana Naphat Somala Montinee Teerarak Nawasit Chotsaeng |
| author_facet | Nutcha Manichart Chamroon Laosinwattana Naphat Somala Montinee Teerarak Nawasit Chotsaeng |
| author_sort | Nutcha Manichart |
| collection | DOAJ |
| description | Abstract Thirteen fungi that produce compounds with herbicidal activities were isolated, identified, and extracted under the assumption that the mechanism of action occurs during seed exposure to the extract. The extracts from all the fungal strains considerably decreased the growth parameters of Amaranthus tricolor L. The EC010 strain extracts showed the greatest effect. Through ITS region gene sequencing methods, the isolated EC010 was identified as a genus of Diaporthe. The results showed a significant (p < 0.05) inhibitory effect of 91.25% on germination and a decrease in shoot and root length by 91.28% and 95.30%, respectively. The mycelium of Diaporthe sp. was extracted using sequential extraction techniques for the partial separation of the herbicidal fraction. According to the bioassay activities, the EtOAc fraction showed the highest inhibitory activity. The osmotic stress of the A. tricolor seeds was studied. Although the extract increased the accumulation of proline and soluble protein, the treated seeds showed lower imbibition. While the activity of α-amylase was dramatically decreased after treatment. A cytogenetic assay in the treated Allium cepa L. root revealed a decrease in the mitotic index, an altered mitotic phase index, and a promotion of mitotic abnormalities. Accordingly, the Diaporthe sp. may serve as a potential herbicidal compound resource. |
| first_indexed | 2024-03-11T12:41:34Z |
| format | Article |
| id | doaj.art-3f835029074446909b7f9bc24d5a017a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T12:41:34Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
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| spelling | doaj.art-3f835029074446909b7f9bc24d5a017a2023-11-05T12:17:36ZengNature PortfolioScientific Reports2045-23222023-10-0113111210.1038/s41598-023-46201-0Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L.Nutcha Manichart0Chamroon Laosinwattana1Naphat Somala2Montinee Teerarak3Nawasit Chotsaeng4Department of Plant Production Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology LadkrabangDepartment of Plant Production Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology LadkrabangDepartment of Plant Production Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology LadkrabangDepartment of Plant Production Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology LadkrabangDepartment of Chemistry, School of Science, King Mongkut’s Institute of Technology LadkrabangAbstract Thirteen fungi that produce compounds with herbicidal activities were isolated, identified, and extracted under the assumption that the mechanism of action occurs during seed exposure to the extract. The extracts from all the fungal strains considerably decreased the growth parameters of Amaranthus tricolor L. The EC010 strain extracts showed the greatest effect. Through ITS region gene sequencing methods, the isolated EC010 was identified as a genus of Diaporthe. The results showed a significant (p < 0.05) inhibitory effect of 91.25% on germination and a decrease in shoot and root length by 91.28% and 95.30%, respectively. The mycelium of Diaporthe sp. was extracted using sequential extraction techniques for the partial separation of the herbicidal fraction. According to the bioassay activities, the EtOAc fraction showed the highest inhibitory activity. The osmotic stress of the A. tricolor seeds was studied. Although the extract increased the accumulation of proline and soluble protein, the treated seeds showed lower imbibition. While the activity of α-amylase was dramatically decreased after treatment. A cytogenetic assay in the treated Allium cepa L. root revealed a decrease in the mitotic index, an altered mitotic phase index, and a promotion of mitotic abnormalities. Accordingly, the Diaporthe sp. may serve as a potential herbicidal compound resource.https://doi.org/10.1038/s41598-023-46201-0 |
| spellingShingle | Nutcha Manichart Chamroon Laosinwattana Naphat Somala Montinee Teerarak Nawasit Chotsaeng Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L. Scientific Reports |
| title | Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L. |
| title_full | Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L. |
| title_fullStr | Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L. |
| title_full_unstemmed | Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L. |
| title_short | Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L. |
| title_sort | physiological mechanism of action and partial separation of herbicide active compounds from the diaporthe sp extract on amaranthus tricolor l |
| url | https://doi.org/10.1038/s41598-023-46201-0 |
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