Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm
Cryodamage affects the normal physiological functions and survivability of boar sperm during cryopreservation. Lysine acetylation is thought to be an important regulatory mechanism in sperm functions. However, little is known about protein acetylation and its effects on cryotolerance or cryodamage i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/10983 |
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| author | Malik Ahsan Ali Ziyue Qin Shan Dou Anqi Huang Yihan Wang Xiang Yuan Yan Zhang Qingyong Ni Rameesha Azmat Changjun Zeng |
| author_facet | Malik Ahsan Ali Ziyue Qin Shan Dou Anqi Huang Yihan Wang Xiang Yuan Yan Zhang Qingyong Ni Rameesha Azmat Changjun Zeng |
| author_sort | Malik Ahsan Ali |
| collection | DOAJ |
| description | Cryodamage affects the normal physiological functions and survivability of boar sperm during cryopreservation. Lysine acetylation is thought to be an important regulatory mechanism in sperm functions. However, little is known about protein acetylation and its effects on cryotolerance or cryodamage in boar sperm. In this study, the characterization and protein acetylation dynamics of boar sperm during cryopreservation were determined using liquid chromatography–mass spectrometry (LC-MS). A total of 1440 proteins were identified out of 4705 modified proteins, and 2764 quantifiable sites were elucidated. Among the differentially modified sites, 1252 were found to be upregulated compared to 172 downregulated sites in fresh and frozen sperms. Gene ontology indicated that these differentially modified proteins are involved in metabolic processes and catalytic and antioxidant activities, which are involved in pyruvate metabolism, phosphorylation and lysine degradation. In addition, the present study demonstrated that the mRNA and protein expressions of SIRT5, IDH2, MDH2 and LDHC, associated with sperm quality parameters, are downregulated after cryopreservation. In conclusion, cryopreservation induces the acetylation and deacetylation of energy metabolism-related proteins, which may contribute to the post-thawed boar sperm quality parameters. |
| first_indexed | 2024-03-11T01:38:56Z |
| format | Article |
| id | doaj.art-3f8ba25ddab04b81ba9577b6113ed03e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:38:56Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3f8ba25ddab04b81ba9577b6113ed03e2023-11-18T16:46:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124131098310.3390/ijms241310983Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar SpermMalik Ahsan Ali0Ziyue Qin1Shan Dou2Anqi Huang3Yihan Wang4Xiang Yuan5Yan Zhang6Qingyong Ni7Rameesha Azmat8Changjun Zeng9Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Biochemistry, Faculty of Science and Technology, Government College Women University, Faisalabad 38000, PakistanKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaCryodamage affects the normal physiological functions and survivability of boar sperm during cryopreservation. Lysine acetylation is thought to be an important regulatory mechanism in sperm functions. However, little is known about protein acetylation and its effects on cryotolerance or cryodamage in boar sperm. In this study, the characterization and protein acetylation dynamics of boar sperm during cryopreservation were determined using liquid chromatography–mass spectrometry (LC-MS). A total of 1440 proteins were identified out of 4705 modified proteins, and 2764 quantifiable sites were elucidated. Among the differentially modified sites, 1252 were found to be upregulated compared to 172 downregulated sites in fresh and frozen sperms. Gene ontology indicated that these differentially modified proteins are involved in metabolic processes and catalytic and antioxidant activities, which are involved in pyruvate metabolism, phosphorylation and lysine degradation. In addition, the present study demonstrated that the mRNA and protein expressions of SIRT5, IDH2, MDH2 and LDHC, associated with sperm quality parameters, are downregulated after cryopreservation. In conclusion, cryopreservation induces the acetylation and deacetylation of energy metabolism-related proteins, which may contribute to the post-thawed boar sperm quality parameters.https://www.mdpi.com/1422-0067/24/13/10983boarsperm cryopreservationprotein acetylationenergy metabolism |
| spellingShingle | Malik Ahsan Ali Ziyue Qin Shan Dou Anqi Huang Yihan Wang Xiang Yuan Yan Zhang Qingyong Ni Rameesha Azmat Changjun Zeng Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm International Journal of Molecular Sciences boar sperm cryopreservation protein acetylation energy metabolism |
| title | Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm |
| title_full | Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm |
| title_fullStr | Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm |
| title_full_unstemmed | Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm |
| title_short | Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm |
| title_sort | cryopreservation induces acetylation of metabolism related proteins in boar sperm |
| topic | boar sperm cryopreservation protein acetylation energy metabolism |
| url | https://www.mdpi.com/1422-0067/24/13/10983 |
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