Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.
Chikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arri...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-05-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0007332 |
| _version_ | 1797661373110419456 |
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| author | Lais Ceschini Machado Mariana Carolina de Morais-Sobral Tulio de Lima Campos Mylena Ribeiro Pereira Maria de Fátima Pessoa Militão de Albuquerque Clément Gilbert Rafael Freitas Oliveira Franca Gabriel Luz Wallau |
| author_facet | Lais Ceschini Machado Mariana Carolina de Morais-Sobral Tulio de Lima Campos Mylena Ribeiro Pereira Maria de Fátima Pessoa Militão de Albuquerque Clément Gilbert Rafael Freitas Oliveira Franca Gabriel Luz Wallau |
| author_sort | Lais Ceschini Machado |
| collection | DOAJ |
| description | Chikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arrived in Brazil in 2014 through two independent introductions: the Asian/Caribbean genotype entered through the North region and the African ECSA genotype was imported through the Northeast region. Following their initial introduction, both genotypes established their urban cycle among large naive human populations causing several outbreaks in the Americas. Here, we sequenced CHIKV genomes from a recent outbreak in the Northeast region of Brazil, employing an in-house developed Next-Generation Sequencing (NGS) protocol capable of directly detecting multiple known CHIKV genotypes from clinical positive samples. Our results demonstrate that both Asian/Caribbean and ECSA genotypes expanded their ranges, reaching cocirculation in the Northeast region of Brazil. In addition, our NGS data supports the findings of simultaneous infection by these two genotypes, suggesting that coinfection might be more common than previously thought in highly endemic areas. Future efforts to understand CHIKV epidemiology should thus take into consideration the possibility of coinfection by different genotypes in the human population. |
| first_indexed | 2024-03-11T18:43:30Z |
| format | Article |
| id | doaj.art-3f8c568ad0f040df8f53e2991c70776d |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-03-11T18:43:30Z |
| publishDate | 2019-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-3f8c568ad0f040df8f53e2991c70776d2023-10-12T05:32:20ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352019-05-01135e000733210.1371/journal.pntd.0007332Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.Lais Ceschini MachadoMariana Carolina de Morais-SobralTulio de Lima CamposMylena Ribeiro PereiraMaria de Fátima Pessoa Militão de AlbuquerqueClément GilbertRafael Freitas Oliveira FrancaGabriel Luz WallauChikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arrived in Brazil in 2014 through two independent introductions: the Asian/Caribbean genotype entered through the North region and the African ECSA genotype was imported through the Northeast region. Following their initial introduction, both genotypes established their urban cycle among large naive human populations causing several outbreaks in the Americas. Here, we sequenced CHIKV genomes from a recent outbreak in the Northeast region of Brazil, employing an in-house developed Next-Generation Sequencing (NGS) protocol capable of directly detecting multiple known CHIKV genotypes from clinical positive samples. Our results demonstrate that both Asian/Caribbean and ECSA genotypes expanded their ranges, reaching cocirculation in the Northeast region of Brazil. In addition, our NGS data supports the findings of simultaneous infection by these two genotypes, suggesting that coinfection might be more common than previously thought in highly endemic areas. Future efforts to understand CHIKV epidemiology should thus take into consideration the possibility of coinfection by different genotypes in the human population.https://doi.org/10.1371/journal.pntd.0007332 |
| spellingShingle | Lais Ceschini Machado Mariana Carolina de Morais-Sobral Tulio de Lima Campos Mylena Ribeiro Pereira Maria de Fátima Pessoa Militão de Albuquerque Clément Gilbert Rafael Freitas Oliveira Franca Gabriel Luz Wallau Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. PLoS Neglected Tropical Diseases |
| title | Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
| title_full | Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
| title_fullStr | Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
| title_full_unstemmed | Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
| title_short | Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
| title_sort | genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in brazil |
| url | https://doi.org/10.1371/journal.pntd.0007332 |
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