Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with Ethylene
This work presents the transcriptome analysis of green ‘Hayward’ (<i>Actinidia deliciosa</i>) and gold ‘Haegeum’ (<i>Actinidia chinensis</i>) kiwifruit cultivars after treatment with ethylene for three days at 25 °C. Illumina high-throughput sequencing platform was used to se...
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MDPI AG
2022-06-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/6/177 |
| _version_ | 1797411954512363520 |
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| author | Han Ryul Choi Min Woo Baek Cheon Soon Jeong Shimeles Tilahun |
| author_facet | Han Ryul Choi Min Woo Baek Cheon Soon Jeong Shimeles Tilahun |
| author_sort | Han Ryul Choi |
| collection | DOAJ |
| description | This work presents the transcriptome analysis of green ‘Hayward’ (<i>Actinidia deliciosa</i>) and gold ‘Haegeum’ (<i>Actinidia chinensis</i>) kiwifruit cultivars after treatment with ethylene for three days at 25 °C. Illumina high-throughput sequencing platform was used to sequence total mRNAs and the transcriptome gene set was constructed by de novo assembly. A total of 1287 and 1724 unigenes were differentially expressed during the comparison of ethylene treatment with control in green ‘Hayward’ and gold ‘Haegeum’, respectively. From the differentially expressed unigenes, 594 and 906 were upregulated, and 693 and 818 were downregulated in the green and gold kiwifruit cultivars, respectively, when treated with ethylene. We also identified a list of genes that were expressed commonly and exclusively in the green and gold kiwifruit cultivars treated with ethylene. Several genes were expressed differentially during the ripening of kiwifruits, and their cumulative effect brought about the softening- and ripening-related changes. This work also identified and categorized genes related to softening and other changes during ripening. Furthermore, the transcript levels of 12 selected representative genes from the differentially expressed genes (DEGs) identified in the transcriptome analysis were confirmed via quantitative real-time PCR (qRT-PCR) to validate the reliability of the expression profiles obtained from RNA-Seq. The data obtained from the present study will add to the information available on the molecular mechanisms of the effects of ethylene during the ripening of kiwifruits. This study will also provide resources for further studies of the genes related to ripening, helping kiwifruit breeders and postharvest technologists to improve ripening quality. |
| first_indexed | 2024-03-09T04:53:53Z |
| format | Article |
| id | doaj.art-3f900f04c7614941b6f9470ca817e608 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T04:53:53Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-3f900f04c7614941b6f9470ca817e6082023-12-03T13:07:50ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-06-014462593261310.3390/cimb44060177Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with EthyleneHan Ryul Choi0Min Woo Baek1Cheon Soon Jeong2Shimeles Tilahun3Department of Horticulture, Kangwon National University, Chuncheon 24341, KoreaDepartment of Horticulture, Kangwon National University, Chuncheon 24341, KoreaDepartment of Horticulture, Kangwon National University, Chuncheon 24341, KoreaDepartment of Horticulture, Kangwon National University, Chuncheon 24341, KoreaThis work presents the transcriptome analysis of green ‘Hayward’ (<i>Actinidia deliciosa</i>) and gold ‘Haegeum’ (<i>Actinidia chinensis</i>) kiwifruit cultivars after treatment with ethylene for three days at 25 °C. Illumina high-throughput sequencing platform was used to sequence total mRNAs and the transcriptome gene set was constructed by de novo assembly. A total of 1287 and 1724 unigenes were differentially expressed during the comparison of ethylene treatment with control in green ‘Hayward’ and gold ‘Haegeum’, respectively. From the differentially expressed unigenes, 594 and 906 were upregulated, and 693 and 818 were downregulated in the green and gold kiwifruit cultivars, respectively, when treated with ethylene. We also identified a list of genes that were expressed commonly and exclusively in the green and gold kiwifruit cultivars treated with ethylene. Several genes were expressed differentially during the ripening of kiwifruits, and their cumulative effect brought about the softening- and ripening-related changes. This work also identified and categorized genes related to softening and other changes during ripening. Furthermore, the transcript levels of 12 selected representative genes from the differentially expressed genes (DEGs) identified in the transcriptome analysis were confirmed via quantitative real-time PCR (qRT-PCR) to validate the reliability of the expression profiles obtained from RNA-Seq. The data obtained from the present study will add to the information available on the molecular mechanisms of the effects of ethylene during the ripening of kiwifruits. This study will also provide resources for further studies of the genes related to ripening, helping kiwifruit breeders and postharvest technologists to improve ripening quality.https://www.mdpi.com/1467-3045/44/6/177kiwifruittranscriptome analysisgene expressionethylene treatmentsoftening |
| spellingShingle | Han Ryul Choi Min Woo Baek Cheon Soon Jeong Shimeles Tilahun Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with Ethylene Current Issues in Molecular Biology kiwifruit transcriptome analysis gene expression ethylene treatment softening |
| title | Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with Ethylene |
| title_full | Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with Ethylene |
| title_fullStr | Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with Ethylene |
| title_full_unstemmed | Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with Ethylene |
| title_short | Comparative Transcriptome Analysis of Softening and Ripening-Related Genes in Kiwifruit Cultivars Treated with Ethylene |
| title_sort | comparative transcriptome analysis of softening and ripening related genes in kiwifruit cultivars treated with ethylene |
| topic | kiwifruit transcriptome analysis gene expression ethylene treatment softening |
| url | https://www.mdpi.com/1467-3045/44/6/177 |
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