Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLD
In the present work, a rapid, accurate, and cost-effective method was developed for the simultaneous quantification of aflatoxins and benzo(a)pyrene in lipid matrices, using solid-phase extraction (SPE) via humic acid-bonded silica (HAS) sorbents, followed by high-performance liquid chromatography c...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/5/352 |
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| author | Di Yuan Liangxiao Zhang Fei Ma Peiwu Li |
| author_facet | Di Yuan Liangxiao Zhang Fei Ma Peiwu Li |
| author_sort | Di Yuan |
| collection | DOAJ |
| description | In the present work, a rapid, accurate, and cost-effective method was developed for the simultaneous quantification of aflatoxins and benzo(a)pyrene in lipid matrices, using solid-phase extraction (SPE) via humic acid-bonded silica (HAS) sorbents, followed by high-performance liquid chromatography coupled with photochemical post-column reactor fluorescence spectroscopy (HPLC–PHRED–FLD) analysis. The major parameters of extraction efficiency and HPLC–PHRED–FLD analysis were investigated and this method was fully validated. The limits of quantification and the limits of detection were 0.05–0.30 and 0.01–0.09 µg kg<sup>−1</sup>, respectively. The recoveries were 66.9%–118.4% with intra-day and inter-day precision less than 7.2%. The results of 80 oil samples from supermarkets indicated a high occurrence of BaP, and most of concentrations were within the requirements of EU and China food safety regulations. This is the first utilization of HAS–SPE HPLC–PHRED–FLD to simultaneously analyze the occurrence of aflatoxins and benzo(a)pyrene in vegetable oils. |
| first_indexed | 2024-03-10T01:41:12Z |
| format | Article |
| id | doaj.art-3f9204e76c064c10ac594361843631d3 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T01:41:12Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-3f9204e76c064c10ac594361843631d32023-11-23T13:23:22ZengMDPI AGToxins2072-66512022-05-0114535210.3390/toxins14050352Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLDDi Yuan0Liangxiao Zhang1Fei Ma2Peiwu Li3Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaIn the present work, a rapid, accurate, and cost-effective method was developed for the simultaneous quantification of aflatoxins and benzo(a)pyrene in lipid matrices, using solid-phase extraction (SPE) via humic acid-bonded silica (HAS) sorbents, followed by high-performance liquid chromatography coupled with photochemical post-column reactor fluorescence spectroscopy (HPLC–PHRED–FLD) analysis. The major parameters of extraction efficiency and HPLC–PHRED–FLD analysis were investigated and this method was fully validated. The limits of quantification and the limits of detection were 0.05–0.30 and 0.01–0.09 µg kg<sup>−1</sup>, respectively. The recoveries were 66.9%–118.4% with intra-day and inter-day precision less than 7.2%. The results of 80 oil samples from supermarkets indicated a high occurrence of BaP, and most of concentrations were within the requirements of EU and China food safety regulations. This is the first utilization of HAS–SPE HPLC–PHRED–FLD to simultaneously analyze the occurrence of aflatoxins and benzo(a)pyrene in vegetable oils.https://www.mdpi.com/2072-6651/14/5/352aflatoxinssolid-phase extraction (SPE)quantificationhigh-performance liquid chromatography with photochemical post-column reactors fluorescence detector (HPLC–PHRED–FLD)vegetable oil |
| spellingShingle | Di Yuan Liangxiao Zhang Fei Ma Peiwu Li Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLD Toxins aflatoxins solid-phase extraction (SPE) quantification high-performance liquid chromatography with photochemical post-column reactors fluorescence detector (HPLC–PHRED–FLD) vegetable oil |
| title | Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLD |
| title_full | Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLD |
| title_fullStr | Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLD |
| title_full_unstemmed | Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLD |
| title_short | Simultaneous Determination of Aflatoxins and Benzo(a)pyrene in Vegetable Oils Using Humic Acid-Bonded Silica SPE HPLC–PHRED–FLD |
| title_sort | simultaneous determination of aflatoxins and benzo a pyrene in vegetable oils using humic acid bonded silica spe hplc phred fld |
| topic | aflatoxins solid-phase extraction (SPE) quantification high-performance liquid chromatography with photochemical post-column reactors fluorescence detector (HPLC–PHRED–FLD) vegetable oil |
| url | https://www.mdpi.com/2072-6651/14/5/352 |
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