Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method
Sex identification of endangered and protected birds in captivity is very important for conservation programs. Half of the world’s bird species are monomorphic, where male and female are difficult to distinguished morphologically, including cockatoos. Sex identification using molecular bird sexing i...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2021-03-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/54611 |
| _version_ | 1811170991636742144 |
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| author | Diana Savitri Irhamna Putri Warih Pulung Nugrahani Medania Purwaningrum Aris Haryanto |
| author_facet | Diana Savitri Irhamna Putri Warih Pulung Nugrahani Medania Purwaningrum Aris Haryanto |
| author_sort | Diana Savitri |
| collection | DOAJ |
| description | Sex identification of endangered and protected birds in captivity is very important for conservation programs. Half of the world’s bird species are monomorphic, where male and female are difficult to distinguished morphologically, including cockatoos. Sex identification using molecular bird sexing is more accurate and applicable because it directly targets the sex chromosomes. The purpose of this study was to determine the sex of Sulphur‐crested cockatoo (Cacatua galerita) by detecting differences in the intron size of the chromodomain helicase DNA‐binding 1 (CHD1) gene on the Z and W chromosomes by polymerase chain reaction (PCR) method and to compare of plucked feathers and blood samples as DNA sources for molecular bird sexing. DNA was extracted from feather and blood samples from four C. galerita. Extracted DNA was amplified on the CHD1 gene by PCR method with P2, MP, and NP primers, which were visualized using agarose gel 1.5% under UV transilluminator with a wavelength of 280 nm. The resulting PCR product was detected at 392 bp for the CHD1 Z gene segment and 297 bp for CHD1 W gene segments, where males showed a single DNA band (ZZ) and females showed a double DNA band (ZW). Four C. galerita were 100% successfully determined, consisting of one female and three males. Electrophoresis results showed DNA bands from blood samples were thicker and brighter than DNA bands from feather samples. |
| first_indexed | 2024-04-10T17:07:08Z |
| format | Article |
| id | doaj.art-3f95eef1c56a4ad0af706cdb8ba348f7 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-10T17:07:08Z |
| publishDate | 2021-03-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-3f95eef1c56a4ad0af706cdb8ba348f72023-02-06T03:00:10ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412021-03-012611610.22146/ijbiotech.5461129521Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction methodDiana Savitri0Irhamna Putri1Warih Pulung Nugrahani2Medania Purwaningrum3Aris Haryanto4Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281Wildlife Rescue Centre (WRC), Jl. Pengasih‐Nanggulan, Pengasih, Kulon Progo, Yogyakarta 55652, IndonesiaWildlife Rescue Centre (WRC), Jl. Pengasih‐Nanggulan, Pengasih, Kulon Progo, Yogyakarta 55652, IndonesiaDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281Sex identification of endangered and protected birds in captivity is very important for conservation programs. Half of the world’s bird species are monomorphic, where male and female are difficult to distinguished morphologically, including cockatoos. Sex identification using molecular bird sexing is more accurate and applicable because it directly targets the sex chromosomes. The purpose of this study was to determine the sex of Sulphur‐crested cockatoo (Cacatua galerita) by detecting differences in the intron size of the chromodomain helicase DNA‐binding 1 (CHD1) gene on the Z and W chromosomes by polymerase chain reaction (PCR) method and to compare of plucked feathers and blood samples as DNA sources for molecular bird sexing. DNA was extracted from feather and blood samples from four C. galerita. Extracted DNA was amplified on the CHD1 gene by PCR method with P2, MP, and NP primers, which were visualized using agarose gel 1.5% under UV transilluminator with a wavelength of 280 nm. The resulting PCR product was detected at 392 bp for the CHD1 Z gene segment and 297 bp for CHD1 W gene segments, where males showed a single DNA band (ZZ) and females showed a double DNA band (ZW). Four C. galerita were 100% successfully determined, consisting of one female and three males. Electrophoresis results showed DNA bands from blood samples were thicker and brighter than DNA bands from feather samples.https://jurnal.ugm.ac.id/ijbiotech/article/view/54611chd1 geneconservationsex identificationz and w chromosomes |
| spellingShingle | Diana Savitri Irhamna Putri Warih Pulung Nugrahani Medania Purwaningrum Aris Haryanto Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method Indonesian Journal of Biotechnology chd1 gene conservation sex identification z and w chromosomes |
| title | Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method |
| title_full | Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method |
| title_fullStr | Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method |
| title_full_unstemmed | Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method |
| title_short | Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method |
| title_sort | molecular bird sexing of sulphur crested cockatoo cacatua galerita by polymerase chain reaction method |
| topic | chd1 gene conservation sex identification z and w chromosomes |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/54611 |
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