An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli
The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in Chinawas expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2005-08-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200508-0002_an-elisa-for-antibodies-to-infectious-bronchitis-virus-based-on-nucleocapsid-protein-produced-in-escherichia-co.php |
| Summary: | The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in Chinawas expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the N-ELISA to evaluate the antibody level of chicken serum. In an experiment to test field samples for antibody detection, the N-ELISA assay shared 95.7% identity of total positive ratio with the commercial ELISA kit. It indicated that the N-ELISA assay, which was safer and easier to prepare than traditional methods, was a good candidate for evaluation of IB vaccine efficiency and virus exposure. |
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| ISSN: | 0375-8427 1805-9392 |