Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging
Abstract Background Near-infrared quantum dots (NIR QDs) are a new class of fluorescent labels with excellent bioimaging features, such as high fluorescence intensity, good fluorescence stability, sufficient electron density, and strong tissue-penetrating ability. For all such features, NIR QDs have...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-018-0346-1 |
| _version_ | 1828273444561616896 |
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| author | Jlenia Brunetti Giulia Riolo Mariangela Gentile Andrea Bernini Eugenio Paccagnini Chiara Falciani Luisa Lozzi Silvia Scali Lorenzo Depau Alessandro Pini Pietro Lupetti Luisa Bracci |
| author_facet | Jlenia Brunetti Giulia Riolo Mariangela Gentile Andrea Bernini Eugenio Paccagnini Chiara Falciani Luisa Lozzi Silvia Scali Lorenzo Depau Alessandro Pini Pietro Lupetti Luisa Bracci |
| author_sort | Jlenia Brunetti |
| collection | DOAJ |
| description | Abstract Background Near-infrared quantum dots (NIR QDs) are a new class of fluorescent labels with excellent bioimaging features, such as high fluorescence intensity, good fluorescence stability, sufficient electron density, and strong tissue-penetrating ability. For all such features, NIR QDs have great potential for early cancer diagnosis, in vivo tumor imaging and high resolution electron microscopy studies on cancer cells. Results In the present study we constructed NIR QDs functionalized with the NT4 cancer-selective tetrabranched peptides (NT4-QDs). We observed specific uptake of NT4-QDs in human cancer cells in in vitro experiments and a much higher selective accumulation and retention of targeted QDs at the tumor site, compared to not targeted QDs, in a colon cancer mouse model. Conclusions NIR QDs labelled with the tetrabranched NT4 peptide have very promising performance for selective addressing of tumor cells in vitro and in vivo, proving rising features of NT4-QDs as theranostics. |
| first_indexed | 2024-04-13T06:20:47Z |
| format | Article |
| id | doaj.art-3fa4334a5d7749a19cd6a5b86c810471 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T06:20:47Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-3fa4334a5d7749a19cd6a5b86c8104712022-12-22T02:58:38ZengBMCJournal of Nanobiotechnology1477-31552018-03-0116111010.1186/s12951-018-0346-1Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imagingJlenia Brunetti0Giulia Riolo1Mariangela Gentile2Andrea Bernini3Eugenio Paccagnini4Chiara Falciani5Luisa Lozzi6Silvia Scali7Lorenzo Depau8Alessandro Pini9Pietro Lupetti10Luisa Bracci11Department of Medical Biotechnologies, University of SienaDepartment of Medical Biotechnologies, University of SienaDepartment of Life Sciences, University of SienaDepartment of Biotechnology, Chemistry and Pharmacy, University of SienaDepartment of Life Sciences, University of SienaDepartment of Medical Biotechnologies, University of SienaDepartment of Medical Biotechnologies, University of SienaDepartment of Medical Biotechnologies, University of SienaDepartment of Medical Biotechnologies, University of SienaDepartment of Medical Biotechnologies, University of SienaDepartment of Life Sciences, University of SienaDepartment of Medical Biotechnologies, University of SienaAbstract Background Near-infrared quantum dots (NIR QDs) are a new class of fluorescent labels with excellent bioimaging features, such as high fluorescence intensity, good fluorescence stability, sufficient electron density, and strong tissue-penetrating ability. For all such features, NIR QDs have great potential for early cancer diagnosis, in vivo tumor imaging and high resolution electron microscopy studies on cancer cells. Results In the present study we constructed NIR QDs functionalized with the NT4 cancer-selective tetrabranched peptides (NT4-QDs). We observed specific uptake of NT4-QDs in human cancer cells in in vitro experiments and a much higher selective accumulation and retention of targeted QDs at the tumor site, compared to not targeted QDs, in a colon cancer mouse model. Conclusions NIR QDs labelled with the tetrabranched NT4 peptide have very promising performance for selective addressing of tumor cells in vitro and in vivo, proving rising features of NT4-QDs as theranostics.http://link.springer.com/article/10.1186/s12951-018-0346-1PeptidesNear-infrared quantum dotsIn vivo imaging |
| spellingShingle | Jlenia Brunetti Giulia Riolo Mariangela Gentile Andrea Bernini Eugenio Paccagnini Chiara Falciani Luisa Lozzi Silvia Scali Lorenzo Depau Alessandro Pini Pietro Lupetti Luisa Bracci Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging Journal of Nanobiotechnology Peptides Near-infrared quantum dots In vivo imaging |
| title | Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging |
| title_full | Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging |
| title_fullStr | Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging |
| title_full_unstemmed | Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging |
| title_short | Near-infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging |
| title_sort | near infrared quantum dots labelled with a tumor selective tetrabranched peptide for in vivo imaging |
| topic | Peptides Near-infrared quantum dots In vivo imaging |
| url | http://link.springer.com/article/10.1186/s12951-018-0346-1 |
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