The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approach
Societal Impact Statement The English West Country is the home of cider making, providing the region with jobs and industry, as well as cultural reference points such as Laurie Lee's Cider with Rosie. Many important cider apple varieties were developed at Long Ashton Research Station (LARS), ne...
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| Format: | Article |
| Language: | English |
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Wiley
2020-03-01
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| Series: | Plants, People, Planet |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ppp3.10074 |
| _version_ | 1818282584255234048 |
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| author | Helen Harper Mark O. Winfield Liz Copas Sacha A. Przewieslik‐Allen Gary L. A. Barker Amanda Burridge Bob R. Hughes Les Davies Keith J. Edwards |
| author_facet | Helen Harper Mark O. Winfield Liz Copas Sacha A. Przewieslik‐Allen Gary L. A. Barker Amanda Burridge Bob R. Hughes Les Davies Keith J. Edwards |
| author_sort | Helen Harper |
| collection | DOAJ |
| description | Societal Impact Statement The English West Country is the home of cider making, providing the region with jobs and industry, as well as cultural reference points such as Laurie Lee's Cider with Rosie. Many important cider apple varieties were developed at Long Ashton Research Station (LARS), near Bristol, UK, including 29 varieties known collectively as ‘The Girls’. After its closure, some of the knowledge and expertise acquired at Long Ashton was lost, including the pedigree of ‘The Girls’. We sampled LARS’ derived trees and, using a novel genotyping technique, rediscovered the pedigree of ‘The Girls’, ensuring that this important cider apple collection will be available for future generations. Summary Our research had two objectives: (a) record the influence of Long Ashton Research Station on the introduction of new cider apple cultivars to the UK; (b) rediscover the parentage of the cider apple cultivars known collectively as ‘The Girls’. For rapid, cost effective and accurate genotyping, we used the recently developed, medium density, single nucleotide polymorphism‐based genotyping procedure, SEQSNP®, to characterize the cultivars. We generated a medium density (1,500 markers), whole genome genotype for 245 apple cultivars that allowed us to determine the relationship between cultivars and, in so doing, rediscover the parentage of ‘The Girls’. We show that SNP genotyping is an efficient tool for the analysis of genetic diversity in cider apples and apples in general, and that the cider apple breeding programme carried out at Long Ashton Research Station made, and continues to make, a unique contribution to UK cider production. |
| first_indexed | 2024-12-13T00:23:20Z |
| format | Article |
| id | doaj.art-3fac8a502eed4f439f8867a5ff2c3d2c |
| institution | Directory Open Access Journal |
| issn | 2572-2611 |
| language | English |
| last_indexed | 2024-12-13T00:23:20Z |
| publishDate | 2020-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plants, People, Planet |
| spelling | doaj.art-3fac8a502eed4f439f8867a5ff2c3d2c2022-12-22T00:05:29ZengWileyPlants, People, Planet2572-26112020-03-012216717510.1002/ppp3.10074The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approachHelen Harper0Mark O. Winfield1Liz Copas2Sacha A. Przewieslik‐Allen3Gary L. A. Barker4Amanda Burridge5Bob R. Hughes6Les Davies7Keith J. Edwards8School of Biological Sciences University of Bristol Bristol UKSchool of Biological Sciences University of Bristol Bristol UKLullingstone Fore Street WinshamSomerset UKSchool of Biological Sciences University of Bristol Bristol UKSchool of Biological Sciences University of Bristol Bristol UKSchool of Biological Sciences University of Bristol Bristol UK7 Warren Lane Long AshtonBristol UK4 Springfield Terrace StreetSomerset UKSchool of Biological Sciences University of Bristol Bristol UKSocietal Impact Statement The English West Country is the home of cider making, providing the region with jobs and industry, as well as cultural reference points such as Laurie Lee's Cider with Rosie. Many important cider apple varieties were developed at Long Ashton Research Station (LARS), near Bristol, UK, including 29 varieties known collectively as ‘The Girls’. After its closure, some of the knowledge and expertise acquired at Long Ashton was lost, including the pedigree of ‘The Girls’. We sampled LARS’ derived trees and, using a novel genotyping technique, rediscovered the pedigree of ‘The Girls’, ensuring that this important cider apple collection will be available for future generations. Summary Our research had two objectives: (a) record the influence of Long Ashton Research Station on the introduction of new cider apple cultivars to the UK; (b) rediscover the parentage of the cider apple cultivars known collectively as ‘The Girls’. For rapid, cost effective and accurate genotyping, we used the recently developed, medium density, single nucleotide polymorphism‐based genotyping procedure, SEQSNP®, to characterize the cultivars. We generated a medium density (1,500 markers), whole genome genotype for 245 apple cultivars that allowed us to determine the relationship between cultivars and, in so doing, rediscover the parentage of ‘The Girls’. We show that SNP genotyping is an efficient tool for the analysis of genetic diversity in cider apples and apples in general, and that the cider apple breeding programme carried out at Long Ashton Research Station made, and continues to make, a unique contribution to UK cider production.https://doi.org/10.1002/ppp3.10074applecidergenotypingLong Ashton Research StationSEQSNP®SNP |
| spellingShingle | Helen Harper Mark O. Winfield Liz Copas Sacha A. Przewieslik‐Allen Gary L. A. Barker Amanda Burridge Bob R. Hughes Les Davies Keith J. Edwards The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approach Plants, People, Planet apple cider genotyping Long Ashton Research Station SEQSNP® SNP |
| title | The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approach |
| title_full | The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approach |
| title_fullStr | The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approach |
| title_full_unstemmed | The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approach |
| title_short | The Long Ashton Legacy: Characterising United Kingdom West Country cider apples using a genotyping by targeted sequencing approach |
| title_sort | long ashton legacy characterising united kingdom west country cider apples using a genotyping by targeted sequencing approach |
| topic | apple cider genotyping Long Ashton Research Station SEQSNP® SNP |
| url | https://doi.org/10.1002/ppp3.10074 |
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