DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines
The Philippines hosts a wide-ranging diversity of plant species with pharmacological potential. Species identification on the basis of DNA sequences has been done for some time in plants and used to detect variations among the sequences specifying genetic divergence as a result of molecular evolutio...
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| Format: | Article |
| Language: | English |
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International Journal of Secondary Metabolite
2018-12-01
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| Series: | International Journal of Secondary Metabolite |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/39967/493809?publisher=ijate |
| _version_ | 1797914569630285824 |
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| author | John Dave C. Aquino Lariza Mae Pajarillaga Jerwin R. Undan |
| author_facet | John Dave C. Aquino Lariza Mae Pajarillaga Jerwin R. Undan |
| author_sort | John Dave C. Aquino |
| collection | DOAJ |
| description | The
Philippines hosts a wide-ranging diversity of plant species with
pharmacological potential. Species identification on the basis of DNA sequences
has been done for some time in plants and used to detect variations among the
sequences specifying genetic divergence as a result of molecular evolution during
the course of time. In this study, molecular approach as well as phylogentic
analysis were conducted to identify the wild plant "Lal lat tan"
collected from Imugan, Sta Fe, Nueva Vizcaya. In addition, thin layer
chromatography was conducted to determine the bioactive compounds present in
the wild plant. For molecular approach, the genomic DNA was extracted from the
young leaves using CTAB and amplified using the nrDNA ITS marker. The PCR
amplified product was sequenced and subjected for search query analysis using
BLAST and was identified as Dendrocnide
meyeniana. Using chloroform+methanol as solvent system, the result revealed
the presence of saponins, phenols, tannins, flavonoids, anthrones,
anthraquinones, terpenes and steroids which are considered as active medicinal
phytochemical constituents. Lal lat tan is a wild plant and was known for
having stinger leaf that lead to severe itchiness and swelling of eyes and face
when touched. |
| first_indexed | 2024-04-10T12:28:36Z |
| format | Article |
| id | doaj.art-3fafb8db3dfe48ce8e01384f851c5cbf |
| institution | Directory Open Access Journal |
| issn | 2148-6905 |
| language | English |
| last_indexed | 2024-04-10T12:28:36Z |
| publishDate | 2018-12-01 |
| publisher | International Journal of Secondary Metabolite |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj.art-3fafb8db3dfe48ce8e01384f851c5cbf2023-02-15T16:15:03ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052018-12-015435336110.21448/ijsm.493809618DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, PhilippinesJohn Dave C. AquinoLariza Mae PajarillagaJerwin R. UndanThe Philippines hosts a wide-ranging diversity of plant species with pharmacological potential. Species identification on the basis of DNA sequences has been done for some time in plants and used to detect variations among the sequences specifying genetic divergence as a result of molecular evolution during the course of time. In this study, molecular approach as well as phylogentic analysis were conducted to identify the wild plant "Lal lat tan" collected from Imugan, Sta Fe, Nueva Vizcaya. In addition, thin layer chromatography was conducted to determine the bioactive compounds present in the wild plant. For molecular approach, the genomic DNA was extracted from the young leaves using CTAB and amplified using the nrDNA ITS marker. The PCR amplified product was sequenced and subjected for search query analysis using BLAST and was identified as Dendrocnide meyeniana. Using chloroform+methanol as solvent system, the result revealed the presence of saponins, phenols, tannins, flavonoids, anthrones, anthraquinones, terpenes and steroids which are considered as active medicinal phytochemical constituents. Lal lat tan is a wild plant and was known for having stinger leaf that lead to severe itchiness and swelling of eyes and face when touched.https://dergipark.org.tr/tr/pub/ijsm/issue/39967/493809?publisher=ijateitslal lat tandendrocnide meyeniana |
| spellingShingle | John Dave C. Aquino Lariza Mae Pajarillaga Jerwin R. Undan DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines International Journal of Secondary Metabolite its lal lat tan dendrocnide meyeniana |
| title | DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines |
| title_full | DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines |
| title_fullStr | DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines |
| title_full_unstemmed | DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines |
| title_short | DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines |
| title_sort | dna barcoding and phytochemical profiling of wild plant lal lat tan from imugan sta fe nueva vizcaya philippines |
| topic | its lal lat tan dendrocnide meyeniana |
| url | https://dergipark.org.tr/tr/pub/ijsm/issue/39967/493809?publisher=ijate |
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