Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation
NaHCO3 accelerates the aging of tobacco leaves; however, the underlying molecular mechanisms have not been elucidated. This study aimed to explore the mechanism of NaHCO3 in the promotion of tobacco leaf maturation using transcriptome analysis. Leaves on plants or detached leaves of the tobacco vari...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-04-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-0849 |
| _version_ | 1797197594837909504 |
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| author | Wang Tingting Zhao Yuanyuan Wang Dexun Shi Hongzhi |
| author_facet | Wang Tingting Zhao Yuanyuan Wang Dexun Shi Hongzhi |
| author_sort | Wang Tingting |
| collection | DOAJ |
| description | NaHCO3 accelerates the aging of tobacco leaves; however, the underlying molecular mechanisms have not been elucidated. This study aimed to explore the mechanism of NaHCO3 in the promotion of tobacco leaf maturation using transcriptome analysis. Leaves on plants or detached leaves of the tobacco variety, Honghua Dajinyuan, were sprayed with or without 1% NaHCO3. The leaf yellowing was observed, the pigment content and enzyme activities were determined and RNA sequencing (RNA-seq) was performed. Spraying NaHCO3 onto detached leaves was found to promote leaf yellowing. Pigment content, catalase activity, and superoxide dismutase activity significantly decreased, whereas peroxidase activity and malondialdehyde content significantly increased. RNA-seq demonstrated that spraying with NaHCO3 upregulated genes associated with cysteine and methionine metabolism; alpha-linolenic acid metabolism; and phenylalanine, tyrosine, and tryptophan biosynthesis and downregulated genes related to photosynthesis and carotenoid biosynthesis. Genes correlated with autophagy-other, valine, leucine, and isoleucine degradation, and the MAPK signaling pathway were upregulated while those correlated with DNA replication, phenylalanine, and tyrosine and tryptophan biosynthesis were downregulated in detached leaves sprayed with NaHCO3 compared with the plant leaves sprayed with NaHCO3. Overall, this study is the first to elucidate the molecular and metabolic mechanisms of NaHCO3 in the promotion of tobacco leaf maturation. |
| first_indexed | 2024-04-24T06:46:27Z |
| format | Article |
| id | doaj.art-3fb012cc415b4da7b15d8457eb61d8ef |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-04-24T06:46:27Z |
| publishDate | 2024-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-3fb012cc415b4da7b15d8457eb61d8ef2024-04-22T19:39:30ZengDe GruyterOpen Life Sciences2391-54122024-04-0119112110.1515/biol-2022-0849Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturationWang Tingting0Zhao Yuanyuan1Wang Dexun2Shi Hongzhi3College of Tobacco Science, Henan Agricultural University, No. 218 Pingan Avenue, Zhengdong New District, Zhengzhou, Henan, 450046, ChinaCollege of Tobacco Science, Henan Agricultural University, No. 218 Pingan Avenue, Zhengdong New District, Zhengzhou, Henan, 450046, ChinaDali Branch of Yunnan Provincial Tobacco Company, Dali, Yunnan, 671000, ChinaCollege of Tobacco Science, Henan Agricultural University, No. 218 Pingan Avenue, Zhengdong New District, Zhengzhou, Henan, 450046, ChinaNaHCO3 accelerates the aging of tobacco leaves; however, the underlying molecular mechanisms have not been elucidated. This study aimed to explore the mechanism of NaHCO3 in the promotion of tobacco leaf maturation using transcriptome analysis. Leaves on plants or detached leaves of the tobacco variety, Honghua Dajinyuan, were sprayed with or without 1% NaHCO3. The leaf yellowing was observed, the pigment content and enzyme activities were determined and RNA sequencing (RNA-seq) was performed. Spraying NaHCO3 onto detached leaves was found to promote leaf yellowing. Pigment content, catalase activity, and superoxide dismutase activity significantly decreased, whereas peroxidase activity and malondialdehyde content significantly increased. RNA-seq demonstrated that spraying with NaHCO3 upregulated genes associated with cysteine and methionine metabolism; alpha-linolenic acid metabolism; and phenylalanine, tyrosine, and tryptophan biosynthesis and downregulated genes related to photosynthesis and carotenoid biosynthesis. Genes correlated with autophagy-other, valine, leucine, and isoleucine degradation, and the MAPK signaling pathway were upregulated while those correlated with DNA replication, phenylalanine, and tyrosine and tryptophan biosynthesis were downregulated in detached leaves sprayed with NaHCO3 compared with the plant leaves sprayed with NaHCO3. Overall, this study is the first to elucidate the molecular and metabolic mechanisms of NaHCO3 in the promotion of tobacco leaf maturation.https://doi.org/10.1515/biol-2022-0849tobacconahco3 rna sequencingleaf maturation |
| spellingShingle | Wang Tingting Zhao Yuanyuan Wang Dexun Shi Hongzhi Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation Open Life Sciences tobacco nahco3 rna sequencing leaf maturation |
| title | Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation |
| title_full | Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation |
| title_fullStr | Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation |
| title_full_unstemmed | Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation |
| title_short | Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation |
| title_sort | transcriptome analysis reveals the mechanism of nahco3 promoting tobacco leaf maturation |
| topic | tobacco nahco3 rna sequencing leaf maturation |
| url | https://doi.org/10.1515/biol-2022-0849 |
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