Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb
Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-11-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913603706 |
| _version_ | 1818739083431641088 |
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| author | Peng WU Zhi-wei QIN Tao WU Xiu-yan ZHOU Ming XIN Qian-qian GUO |
| author_facet | Peng WU Zhi-wei QIN Tao WU Xiu-yan ZHOU Ming XIN Qian-qian GUO |
| author_sort | Peng WU |
| collection | DOAJ |
| description | Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes (glutathione reductase, GR; glutathione S-tramsferase, GST) and soluble sugar content of cucumber fruit were significantly increased, but malonaldehyde (MDA) content was significantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis (2-DE) protein profiles of control and propamocarb-treated fruits, and identified 18 differentially expressed (13 up-regulated and 5 down-regulated) proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction (qRT-PCR) was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment. |
| first_indexed | 2024-12-18T01:19:12Z |
| format | Article |
| id | doaj.art-3fb3824449584c2cbd32c80d0bd49d73 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T01:19:12Z |
| publishDate | 2013-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-3fb3824449584c2cbd32c80d0bd49d732022-12-21T21:25:53ZengElsevierJournal of Integrative Agriculture2095-31192013-11-01121120222035Proteomic Analysis of Cucumber Defense Responses Induced by PropamocarbPeng WU0Zhi-wei QIN1Tao WU2Xiu-yan ZHOU3Ming XIN4Qian-qian GUO5College of Horticulture, Northeast Agricultural University, Harbin 150030, P.R. ChinaCorrespondence QIN Zhi-wei, Tel: +86-451-55190058, Fax: +86-451-55190058; College of Horticulture, Northeast Agricultural University, Harbin 150030, P.R. ChinaCollege of Horticulture, Northeast Agricultural University, Harbin 150030, P.R. ChinaCollege of Horticulture, Northeast Agricultural University, Harbin 150030, P.R. ChinaCollege of Horticulture, Northeast Agricultural University, Harbin 150030, P.R. ChinaCollege of Horticulture, Northeast Agricultural University, Harbin 150030, P.R. ChinaPropamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes (glutathione reductase, GR; glutathione S-tramsferase, GST) and soluble sugar content of cucumber fruit were significantly increased, but malonaldehyde (MDA) content was significantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis (2-DE) protein profiles of control and propamocarb-treated fruits, and identified 18 differentially expressed (13 up-regulated and 5 down-regulated) proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction (qRT-PCR) was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment.http://www.sciencedirect.com/science/article/pii/S2095311913603706cucumberfungicidepropamocarbproteomicsqRT-PCR |
| spellingShingle | Peng WU Zhi-wei QIN Tao WU Xiu-yan ZHOU Ming XIN Qian-qian GUO Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb Journal of Integrative Agriculture cucumber fungicide propamocarb proteomics qRT-PCR |
| title | Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb |
| title_full | Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb |
| title_fullStr | Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb |
| title_full_unstemmed | Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb |
| title_short | Proteomic Analysis of Cucumber Defense Responses Induced by Propamocarb |
| title_sort | proteomic analysis of cucumber defense responses induced by propamocarb |
| topic | cucumber fungicide propamocarb proteomics qRT-PCR |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913603706 |
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