Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain
The generation of reactive oxygen species (ROS) and scavenging of the superoxide radical by superoxide dismutase (SOD) was studied in mat-like canopies of the green macroalga Ulva rotundata Bliding in a tidal brine pond system in southern Spain. Artificial canopies were covered with different cut-of...
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| Format: | Article |
| Language: | English |
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Consejo Superior de Investigaciones Científicas
2003-09-01
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| Series: | Scientia Marina |
| Subjects: | |
| Online Access: | http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/485 |
| _version_ | 1818737185967308800 |
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| author | Kai Bischof Paul J. Janknegt Anita G.J. Buma Jan W. Rijstenbil Gloria Peralta Anneke M. Breeman |
| author_facet | Kai Bischof Paul J. Janknegt Anita G.J. Buma Jan W. Rijstenbil Gloria Peralta Anneke M. Breeman |
| author_sort | Kai Bischof |
| collection | DOAJ |
| description | The generation of reactive oxygen species (ROS) and scavenging of the superoxide radical by superoxide dismutase (SOD) was studied in mat-like canopies of the green macroalga Ulva rotundata Bliding in a tidal brine pond system in southern Spain. Artificial canopies were covered with different cut-off filters, generating different radiation conditions. ROS and SOD were assessed after three days of exposure. ROS induced lipid peroxidation depended on the position of individual thalli within the canopy and on radiation conditions. Samples exposed to the full solar spectrum were most affected, whereas samples either exposed to photosynthetically active radiation (PAR) alone or UV radiation without PAR exhibited fewer peroxidation products. The activity of SOD appeared to be controlled by the impinging UV-A and UV-B radiation and also increased in response to oxidative stress. The results provide evidence for additive effects of high PAR and UV-B under field conditions and support the previously proposed hypothesis that UV-B effects are mediated by an inhibition of the xanthophyll cycle, which increases ROS production and, consequently, causes oxidative damage to components of the photosynthetic machinery, such as proteins and pigments. |
| first_indexed | 2024-12-18T00:49:02Z |
| format | Article |
| id | doaj.art-3fb53d1459cd46f1a95405c83aba102f |
| institution | Directory Open Access Journal |
| issn | 0214-8358 1886-8134 |
| language | English |
| last_indexed | 2024-12-18T00:49:02Z |
| publishDate | 2003-09-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Scientia Marina |
| spelling | doaj.art-3fb53d1459cd46f1a95405c83aba102f2022-12-21T21:26:44ZengConsejo Superior de Investigaciones CientíficasScientia Marina0214-83581886-81342003-09-0167335335910.3989/scimar.2003.67n3353480Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern SpainKai Bischof0Paul J. Janknegt1Anita G.J. Buma2Jan W. Rijstenbil3Gloria Peralta4Anneke M. Breeman5University of Groningen, Department of Marine BiologyUniversity of Groningen, Department of Marine BiologyUniversity of Groningen, Department of Marine BiologyNetherlands Institute of Ecology, Centre for Estuarine and Coastal ResearchNetherlands Institute of Ecology, Centre for Estuarine and Coastal ResearchUniversity of Groningen, Department of Marine BiologyThe generation of reactive oxygen species (ROS) and scavenging of the superoxide radical by superoxide dismutase (SOD) was studied in mat-like canopies of the green macroalga Ulva rotundata Bliding in a tidal brine pond system in southern Spain. Artificial canopies were covered with different cut-off filters, generating different radiation conditions. ROS and SOD were assessed after three days of exposure. ROS induced lipid peroxidation depended on the position of individual thalli within the canopy and on radiation conditions. Samples exposed to the full solar spectrum were most affected, whereas samples either exposed to photosynthetically active radiation (PAR) alone or UV radiation without PAR exhibited fewer peroxidation products. The activity of SOD appeared to be controlled by the impinging UV-A and UV-B radiation and also increased in response to oxidative stress. The results provide evidence for additive effects of high PAR and UV-B under field conditions and support the previously proposed hypothesis that UV-B effects are mediated by an inhibition of the xanthophyll cycle, which increases ROS production and, consequently, causes oxidative damage to components of the photosynthetic machinery, such as proteins and pigments.http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/485macroalgaephotosynthesisreactive oxygen speciessuperoxide dismutaseulvauv radiation |
| spellingShingle | Kai Bischof Paul J. Janknegt Anita G.J. Buma Jan W. Rijstenbil Gloria Peralta Anneke M. Breeman Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain Scientia Marina macroalgae photosynthesis reactive oxygen species superoxide dismutase ulva uv radiation |
| title | Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain |
| title_full | Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain |
| title_fullStr | Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain |
| title_full_unstemmed | Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain |
| title_short | Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain |
| title_sort | oxidative stress and enzymatic scavenging of superoxide radicals induced by solar uv b radiation in ulva canopies from southern spain |
| topic | macroalgae photosynthesis reactive oxygen species superoxide dismutase ulva uv radiation |
| url | http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/485 |
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