Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells
Objective: To explore the possible neuroprotective activities of Humulus japonicus extract against Parkinson’s disease (PD) in a cellular model. Methods: PD was modeled in PC12 cells using 6-hydroxydopamine (6-OHDA). The cell activity, intracellular levels of reactive oxygen species (ROS), anti-oxid...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=5;spage=197;epage=206;aulast=Wang |
| _version_ | 1818161911035854848 |
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| author | Feng Wang Byoung Ok Cho Jae Young Shin Suping Hao Seon Il Jang |
| author_facet | Feng Wang Byoung Ok Cho Jae Young Shin Suping Hao Seon Il Jang |
| author_sort | Feng Wang |
| collection | DOAJ |
| description | Objective: To explore the possible neuroprotective activities of Humulus japonicus extract against Parkinson’s disease (PD) in a cellular model.
Methods: PD was modeled in PC12 cells using 6-hydroxydopamine (6-OHDA). The cell activity, intracellular levels of reactive oxygen species (ROS), anti-oxidative and anti-apoptotic effects, and other related indicators and related signaling pathways were evaluated to elucidate the neuroprotective effects of Humulus japonicus extract.
Results: Humulus japonicus extract exhibited anti-oxidative and anti-apoptotic effects in 6-OHDA-stimulated PC12 cells. It also reduced oxidative stress-induced ROS accumulation; upregulated antioxidant enzymes, such as glutathione, catalase, heme oxidase-1, and 8-oxguanine glycosylase 1; promoted cell survival by decreasing BAX and increasing Bcl-2 and sirtuin 1 expression via the MAPK and/or Nrf2 signaling pathways.
Conclusions: Humulus japonicus extract has antioxidative and anti-apoptotic effects and could be developed as a promising candidate for preventing and treating oxidative stress-related neurodegenerative diseases. |
| first_indexed | 2024-12-11T16:25:17Z |
| format | Article |
| id | doaj.art-3fb72eede68a4af0a24b941fcf1f5a82 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-12-11T16:25:17Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-3fb72eede68a4af0a24b941fcf1f5a822022-12-22T00:58:45ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222022-01-0112519720610.4103/2221-1691.343387Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cellsFeng WangByoung Ok ChoJae Young ShinSuping HaoSeon Il JangObjective: To explore the possible neuroprotective activities of Humulus japonicus extract against Parkinson’s disease (PD) in a cellular model. Methods: PD was modeled in PC12 cells using 6-hydroxydopamine (6-OHDA). The cell activity, intracellular levels of reactive oxygen species (ROS), anti-oxidative and anti-apoptotic effects, and other related indicators and related signaling pathways were evaluated to elucidate the neuroprotective effects of Humulus japonicus extract. Results: Humulus japonicus extract exhibited anti-oxidative and anti-apoptotic effects in 6-OHDA-stimulated PC12 cells. It also reduced oxidative stress-induced ROS accumulation; upregulated antioxidant enzymes, such as glutathione, catalase, heme oxidase-1, and 8-oxguanine glycosylase 1; promoted cell survival by decreasing BAX and increasing Bcl-2 and sirtuin 1 expression via the MAPK and/or Nrf2 signaling pathways. Conclusions: Humulus japonicus extract has antioxidative and anti-apoptotic effects and could be developed as a promising candidate for preventing and treating oxidative stress-related neurodegenerative diseases.http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=5;spage=197;epage=206;aulast=Wanghumulus japonicus; parkinson’s disease; oxidative stress; apoptosis; mapk; pc12 cells; nrf2 |
| spellingShingle | Feng Wang Byoung Ok Cho Jae Young Shin Suping Hao Seon Il Jang Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells Asian Pacific Journal of Tropical Biomedicine humulus japonicus; parkinson’s disease; oxidative stress; apoptosis; mapk; pc12 cells; nrf2 |
| title | Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells |
| title_full | Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells |
| title_fullStr | Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells |
| title_full_unstemmed | Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells |
| title_short | Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells |
| title_sort | humulus japonicus extract alleviates oxidative stress and apoptosis in 6 hydroxydopamine induced pc12 cells |
| topic | humulus japonicus; parkinson’s disease; oxidative stress; apoptosis; mapk; pc12 cells; nrf2 |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=5;spage=197;epage=206;aulast=Wang |
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