The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome
Abstract We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small s...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
|
| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500716&lng=en&tlng=en |
| _version_ | 1818200189674979328 |
|---|---|
| author | Daniella de Sousa Mendes Moreira Alves Gustavo Souza Gonçalves Aline Silva Moraes Luciano Moreira Alves José Rodrigues do Carmo Neto Mariana Machado Hecht Nadjar Nitz Rodrigo Gurgel-Gonçalves Geisa Bernardes Ana Maria de Castro Maria Regina Chalita Marina Clare Vinaud |
| author_facet | Daniella de Sousa Mendes Moreira Alves Gustavo Souza Gonçalves Aline Silva Moraes Luciano Moreira Alves José Rodrigues do Carmo Neto Mariana Machado Hecht Nadjar Nitz Rodrigo Gurgel-Gonçalves Geisa Bernardes Ana Maria de Castro Maria Regina Chalita Marina Clare Vinaud |
| author_sort | Daniella de Sousa Mendes Moreira Alves |
| collection | DOAJ |
| description | Abstract We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. To the best of our knowledge, this is the first report of T4 Acanthamoeba keratitis case from the Midwest region of Brazil. |
| first_indexed | 2024-12-12T02:33:42Z |
| format | Article |
| id | doaj.art-3fc011f6ec8f4a0b988f042391ced23b |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T02:33:42Z |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-3fc011f6ec8f4a0b988f042391ced23b2022-12-22T00:41:20ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-984951571671910.1590/0037-8682-0010-2018S0037-86822018000500716The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcomeDaniella de Sousa Mendes Moreira AlvesGustavo Souza GonçalvesAline Silva MoraesLuciano Moreira AlvesJosé Rodrigues do Carmo NetoMariana Machado HechtNadjar NitzRodrigo Gurgel-GonçalvesGeisa BernardesAna Maria de CastroMaria Regina ChalitaMarina Clare VinaudAbstract We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. To the best of our knowledge, this is the first report of T4 Acanthamoeba keratitis case from the Midwest region of Brazil.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500716&lng=en&tlng=enAcanthamoeba keratitisCulturePCR |
| spellingShingle | Daniella de Sousa Mendes Moreira Alves Gustavo Souza Gonçalves Aline Silva Moraes Luciano Moreira Alves José Rodrigues do Carmo Neto Mariana Machado Hecht Nadjar Nitz Rodrigo Gurgel-Gonçalves Geisa Bernardes Ana Maria de Castro Maria Regina Chalita Marina Clare Vinaud The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome Revista da Sociedade Brasileira de Medicina Tropical Acanthamoeba keratitis Culture PCR |
| title | The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome |
| title_full | The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome |
| title_fullStr | The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome |
| title_full_unstemmed | The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome |
| title_short | The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome |
| title_sort | first acanthamoeba keratitis case in the midwest region of brazil diagnosis genotyping of the parasite and disease outcome |
| topic | Acanthamoeba keratitis Culture PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500716&lng=en&tlng=en |
| work_keys_str_mv | AT danielladesousamendesmoreiraalves thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT gustavosouzagoncalves thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT alinesilvamoraes thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT lucianomoreiraalves thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT joserodriguesdocarmoneto thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT marianamachadohecht thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT nadjarnitz thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT rodrigogurgelgoncalves thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT geisabernardes thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT anamariadecastro thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT mariareginachalita thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT marinaclarevinaud thefirstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT danielladesousamendesmoreiraalves firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT gustavosouzagoncalves firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT alinesilvamoraes firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT lucianomoreiraalves firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT joserodriguesdocarmoneto firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT marianamachadohecht firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT nadjarnitz firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT rodrigogurgelgoncalves firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT geisabernardes firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT anamariadecastro firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT mariareginachalita firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome AT marinaclarevinaud firstacanthamoebakeratitiscaseinthemidwestregionofbrazildiagnosisgenotypingoftheparasiteanddiseaseoutcome |