In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study
Vascular diseases are increasing health problems affecting >25 million individuals in westernized societies. Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | EBioMedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396414000024 |
| _version_ | 1828810651921809408 |
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| author | Michael Olausson Vijay Kumar Kuna Galyna Travnikova Henrik Bäckdahl Pradeep B. Patil Robert Saalman Helena Borg Anders Jeppsson Suchitra Sumitran-Holgersson |
| author_facet | Michael Olausson Vijay Kumar Kuna Galyna Travnikova Henrik Bäckdahl Pradeep B. Patil Robert Saalman Helena Borg Anders Jeppsson Suchitra Sumitran-Holgersson |
| author_sort | Michael Olausson |
| collection | DOAJ |
| description | Vascular diseases are increasing health problems affecting >25 million individuals in westernized societies. Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Here we demonstrate a novel approach to generate transplantable vascular grafts using decellularized allogeneic vascular scaffolds, repopulated with peripheral whole blood (PWB) in vitro in a bioreactor. Circulating, VEGFR-2+/CD45+ and a smaller fraction of VEGFR-2+/CD14+ cells contributed to repopulation of the graft. SEM micrographs showed flat cells on the luminal surface of the grafts consistent with endothelial cells. For clinical validation, two autologous PWB tissue-engineered vein conduits were prepared and successfully used for by-pass procedures in two pediatric patients. These results provide a proof of principle for the generation of transplantable vascular grafts using a simple autologous blood sample, making it clinically feasible globally. |
| first_indexed | 2024-12-12T09:16:11Z |
| format | Article |
| id | doaj.art-3fc135738ffb4b03aecde14ba904edf6 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-12T09:16:11Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-3fc135738ffb4b03aecde14ba904edf62022-12-22T00:29:23ZengElsevierEBioMedicine2352-39642014-11-0111727910.1016/j.ebiom.2014.09.001In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept StudyMichael Olausson0Vijay Kumar Kuna1Galyna Travnikova2Henrik Bäckdahl3Pradeep B. Patil4Robert Saalman5Helena Borg6Anders Jeppsson7Suchitra Sumitran-Holgersson8Department of Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenSP Technical Research Institute of Sweden, Dept. of Chemistry, Materials and Surfaces, SE-50462 Borås, SwedenDepartment of Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Paediatrics, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Paediatrics, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Cardiothoracic Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SwedenVascular diseases are increasing health problems affecting >25 million individuals in westernized societies. Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Here we demonstrate a novel approach to generate transplantable vascular grafts using decellularized allogeneic vascular scaffolds, repopulated with peripheral whole blood (PWB) in vitro in a bioreactor. Circulating, VEGFR-2+/CD45+ and a smaller fraction of VEGFR-2+/CD14+ cells contributed to repopulation of the graft. SEM micrographs showed flat cells on the luminal surface of the grafts consistent with endothelial cells. For clinical validation, two autologous PWB tissue-engineered vein conduits were prepared and successfully used for by-pass procedures in two pediatric patients. These results provide a proof of principle for the generation of transplantable vascular grafts using a simple autologous blood sample, making it clinically feasible globally.http://www.sciencedirect.com/science/article/pii/S2352396414000024Vein conduitsVascular diseasesTissue-engineeringEndothelial precursors |
| spellingShingle | Michael Olausson Vijay Kumar Kuna Galyna Travnikova Henrik Bäckdahl Pradeep B. Patil Robert Saalman Helena Borg Anders Jeppsson Suchitra Sumitran-Holgersson In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study EBioMedicine Vein conduits Vascular diseases Tissue-engineering Endothelial precursors |
| title | In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study |
| title_full | In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study |
| title_fullStr | In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study |
| title_full_unstemmed | In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study |
| title_short | In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study |
| title_sort | in vivo application of tissue engineered veins using autologous peripheral whole blood a proof of concept study |
| topic | Vein conduits Vascular diseases Tissue-engineering Endothelial precursors |
| url | http://www.sciencedirect.com/science/article/pii/S2352396414000024 |
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