The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection
Sophia Ogechi Ekeuku,1 Benjamin Ka Seng Thong,1,2 Adam Quraisiah,1 Fazalda Annuar,1 Alfizah Hanafiah,3 Mohd Fahami Nur Azlina,1 Kok-Yong Chin1 1Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Malaysia; 2Shanghai Jiao Tong University School of Medicine, Shangh...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-12-01
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| Series: | Drug Design, Development and Therapy |
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| Online Access: | https://www.dovepress.com/the-skeletal-effects-of-short-term-triple-therapy-in-a-rat-model-of-ga-peer-reviewed-article-DDDT |
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| author | Ekeuku SO Thong BKS Quraisiah A Annuar F Hanafiah A Nur Azlina MF Chin KY |
| author_facet | Ekeuku SO Thong BKS Quraisiah A Annuar F Hanafiah A Nur Azlina MF Chin KY |
| author_sort | Ekeuku SO |
| collection | DOAJ |
| description | Sophia Ogechi Ekeuku,1 Benjamin Ka Seng Thong,1,2 Adam Quraisiah,1 Fazalda Annuar,1 Alfizah Hanafiah,3 Mohd Fahami Nur Azlina,1 Kok-Yong Chin1 1Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Malaysia; 2Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Department of Medical Microbiology & Immunology Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, MalaysiaCorrespondence: Kok-Yong ChinDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Level 17, Preclinical Building, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, Kuala Lumpur, MalaysiaTel +60 3-9145-9573Email chinkokyong@ppukm.ukm.edu.myPurpose: Triple therapy is the standard therapy to eradicate Helicobacter pylori (H.pylori) infection. Chronic use of proton pump inhibitors (PPIs), a component of triple therapy, is associated with osteoporosis. However, the skeletal effects of short-term triple therapy containing PPI remain elusive. This study aims to determine the skeletal effect of short-term triple therapy in a rat model of gastric ulcer induced by H. pylori.Methods: Three-month-old male Sprague Dawley rats were assigned to normal control, H. pylori-inoculated group (negative control) and H. pylori-inoculated group receiving triple therapy consisting of omeprazole [2.035 mg/kg body weight (b.w)], amoxicillin (102.80 mg/kg b.w) and clarithromycin (51.37 mg/kg b.w) (n=6/group). H. pylori infection developed for four weeks after inoculation, followed by two-week triple therapy. At the end of the treatment period, femoral bones of the rats were harvested for analysis. Bone mineral density and content of the femurs were determined using dual-energy X-ray absorptiometry, while bone strength was measured with a universal mechanical tester.Results: Bone mineral content was significantly lower in the negative control group compared to the triple therapy group (p=0.014). Triple therapy decreased strain (vs negative control, p=0.002) and displacement of the femur (vs normal control, p=0.004; vs untreated control, p=0.005). No significant difference was observed in other parameters among the study groups (p> 0.05).Conclusion: Short-term triple therapy increases bone mineral content but decreases bone strength of rats. Skeletal prophylaxis should be considered for patients on short-term triple therapy containing PPI.Keywords: bone, gastric ulcer, omeprazole, osteoporosis, osteopenia, proton pump inhibitor |
| first_indexed | 2024-12-21T13:12:57Z |
| format | Article |
| id | doaj.art-3fc8856eeccb4a7585fa5f152b12d34f |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-21T13:12:57Z |
| publishDate | 2020-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-3fc8856eeccb4a7585fa5f152b12d34f2022-12-21T19:02:49ZengDove Medical PressDrug Design, Development and Therapy1177-88812020-12-01Volume 145359536659980The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori InfectionEkeuku SOThong BKSQuraisiah AAnnuar FHanafiah ANur Azlina MFChin KYSophia Ogechi Ekeuku,1 Benjamin Ka Seng Thong,1,2 Adam Quraisiah,1 Fazalda Annuar,1 Alfizah Hanafiah,3 Mohd Fahami Nur Azlina,1 Kok-Yong Chin1 1Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Malaysia; 2Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Department of Medical Microbiology & Immunology Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, MalaysiaCorrespondence: Kok-Yong ChinDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Level 17, Preclinical Building, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, Kuala Lumpur, MalaysiaTel +60 3-9145-9573Email chinkokyong@ppukm.ukm.edu.myPurpose: Triple therapy is the standard therapy to eradicate Helicobacter pylori (H.pylori) infection. Chronic use of proton pump inhibitors (PPIs), a component of triple therapy, is associated with osteoporosis. However, the skeletal effects of short-term triple therapy containing PPI remain elusive. This study aims to determine the skeletal effect of short-term triple therapy in a rat model of gastric ulcer induced by H. pylori.Methods: Three-month-old male Sprague Dawley rats were assigned to normal control, H. pylori-inoculated group (negative control) and H. pylori-inoculated group receiving triple therapy consisting of omeprazole [2.035 mg/kg body weight (b.w)], amoxicillin (102.80 mg/kg b.w) and clarithromycin (51.37 mg/kg b.w) (n=6/group). H. pylori infection developed for four weeks after inoculation, followed by two-week triple therapy. At the end of the treatment period, femoral bones of the rats were harvested for analysis. Bone mineral density and content of the femurs were determined using dual-energy X-ray absorptiometry, while bone strength was measured with a universal mechanical tester.Results: Bone mineral content was significantly lower in the negative control group compared to the triple therapy group (p=0.014). Triple therapy decreased strain (vs negative control, p=0.002) and displacement of the femur (vs normal control, p=0.004; vs untreated control, p=0.005). No significant difference was observed in other parameters among the study groups (p> 0.05).Conclusion: Short-term triple therapy increases bone mineral content but decreases bone strength of rats. Skeletal prophylaxis should be considered for patients on short-term triple therapy containing PPI.Keywords: bone, gastric ulcer, omeprazole, osteoporosis, osteopenia, proton pump inhibitorhttps://www.dovepress.com/the-skeletal-effects-of-short-term-triple-therapy-in-a-rat-model-of-ga-peer-reviewed-article-DDDTbonegastric ulceromeprazoleosteoporosisosteopeniaproton pump inhibitor |
| spellingShingle | Ekeuku SO Thong BKS Quraisiah A Annuar F Hanafiah A Nur Azlina MF Chin KY The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection Drug Design, Development and Therapy bone gastric ulcer omeprazole osteoporosis osteopenia proton pump inhibitor |
| title | The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection |
| title_full | The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection |
| title_fullStr | The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection |
| title_full_unstemmed | The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection |
| title_short | The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection |
| title_sort | skeletal effects of short term triple therapy in a rat model of gastric ulcer induced by helicobacter pylori infection |
| topic | bone gastric ulcer omeprazole osteoporosis osteopenia proton pump inhibitor |
| url | https://www.dovepress.com/the-skeletal-effects-of-short-term-triple-therapy-in-a-rat-model-of-ga-peer-reviewed-article-DDDT |
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