Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery
OBJECTIVES: Bacterial and aseptic meningitis after neurosurgery can present similar clinical signs and symptoms. The aims of this study were to develop and test a molecular method to diagnose bacterial meningitis (BM) after neurosurgery. METHODS: A 16S ribosomal RNA gene PCR-based strategy was deve...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier España
2021-01-01
|
| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322021000100220&tlng=en |
| _version_ | 1811229636386881536 |
|---|---|
| author | Lauro Vieira Perdigão Neto Micheli Medeiros Suzete Cleusa Ferreira Anna Shoko Nishiya Denise Brandão de Assis Ícaro Boszczowski Silvia Figueiredo Costa Anna S. Levin |
| author_facet | Lauro Vieira Perdigão Neto Micheli Medeiros Suzete Cleusa Ferreira Anna Shoko Nishiya Denise Brandão de Assis Ícaro Boszczowski Silvia Figueiredo Costa Anna S. Levin |
| author_sort | Lauro Vieira Perdigão Neto |
| collection | DOAJ |
| description | OBJECTIVES: Bacterial and aseptic meningitis after neurosurgery can present similar clinical signs and symptoms. The aims of this study were to develop and test a molecular method to diagnose bacterial meningitis (BM) after neurosurgery. METHODS: A 16S ribosomal RNA gene PCR-based strategy was developed using artificially inoculated cerebrospinal fluid (CSF) followed by sequencing. The method was tested using CSF samples from 43 patients who had undergone neurosurgery and were suspected to suffer from meningitis, and from 8 patients without neurosurgery or meningitis. Patients were classified into five groups, confirmed BM, probable BM, possible BM, unlikely BM, and no meningitis. RESULTS: Among the samples from the 51 patients, 21 samples (41%) were culture-negative and PCR-positive. Of these, 3 (14%) were probable BM, 4 (19%) were possible BM, 13 (62%) were unlikely BM, and 1 (5%) was meningitis negative. Enterobacterales, non-fermenters (Pseudomonas aeruginosa and Acinetobacter baumannii), Staphylococcus haemolyticus, Granulicatella, Variovorax, and Enterococcus cecorum could be identified. In the group of patients with meningitis, a good agreement (3 of 4) was observed with the results of cultures, including the identification of species. CONCLUSION: Molecular methods may complement the diagnosis, guide treatment, and identify non-cultivable microorganisms. We suggest the association of methods for suspected cases of BM after neurosurgery, especially for instances in which the culture is negative. |
| first_indexed | 2024-04-12T10:16:43Z |
| format | Article |
| id | doaj.art-3fcc0a74bbcd4e0d80b25983fce01884 |
| institution | Directory Open Access Journal |
| issn | 1980-5322 |
| language | English |
| last_indexed | 2024-04-12T10:16:43Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-3fcc0a74bbcd4e0d80b25983fce018842022-12-22T03:37:11ZengElsevier EspañaClinics1980-53222021-01-017610.6061/clinics/2021/e2284Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgeryLauro Vieira Perdigão Netohttps://orcid.org/0000-0002-7681-8090Micheli Medeiroshttps://orcid.org/0000-0001-6870-8898Suzete Cleusa Ferreirahttps://orcid.org/0000-0002-4190-0238Anna Shoko Nishiyahttps://orcid.org/0000-0002-0623-1999Denise Brandão de Assishttps://orcid.org/0000-0002-8758-5472Ícaro Boszczowskihttps://orcid.org/0000-0002-0221-3814Silvia Figueiredo Costahttps://orcid.org/0000-0003-1087-752XAnna S. Levinhttps://orcid.org/0000-0003-2427-8368OBJECTIVES: Bacterial and aseptic meningitis after neurosurgery can present similar clinical signs and symptoms. The aims of this study were to develop and test a molecular method to diagnose bacterial meningitis (BM) after neurosurgery. METHODS: A 16S ribosomal RNA gene PCR-based strategy was developed using artificially inoculated cerebrospinal fluid (CSF) followed by sequencing. The method was tested using CSF samples from 43 patients who had undergone neurosurgery and were suspected to suffer from meningitis, and from 8 patients without neurosurgery or meningitis. Patients were classified into five groups, confirmed BM, probable BM, possible BM, unlikely BM, and no meningitis. RESULTS: Among the samples from the 51 patients, 21 samples (41%) were culture-negative and PCR-positive. Of these, 3 (14%) were probable BM, 4 (19%) were possible BM, 13 (62%) were unlikely BM, and 1 (5%) was meningitis negative. Enterobacterales, non-fermenters (Pseudomonas aeruginosa and Acinetobacter baumannii), Staphylococcus haemolyticus, Granulicatella, Variovorax, and Enterococcus cecorum could be identified. In the group of patients with meningitis, a good agreement (3 of 4) was observed with the results of cultures, including the identification of species. CONCLUSION: Molecular methods may complement the diagnosis, guide treatment, and identify non-cultivable microorganisms. We suggest the association of methods for suspected cases of BM after neurosurgery, especially for instances in which the culture is negative.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322021000100220&tlng=enMeningitis, BacterialCraniotomyCerebrospinal FluidDiagnosisRNA, Ribosomal, 16SSequence Analysis |
| spellingShingle | Lauro Vieira Perdigão Neto Micheli Medeiros Suzete Cleusa Ferreira Anna Shoko Nishiya Denise Brandão de Assis Ícaro Boszczowski Silvia Figueiredo Costa Anna S. Levin Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery Clinics Meningitis, Bacterial Craniotomy Cerebrospinal Fluid Diagnosis RNA, Ribosomal, 16S Sequence Analysis |
| title | Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery |
| title_full | Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery |
| title_fullStr | Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery |
| title_full_unstemmed | Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery |
| title_short | Polymerase chain reaction targeting 16S ribosomal RNA for the diagnosis of bacterial meningitis after neurosurgery |
| title_sort | polymerase chain reaction targeting 16s ribosomal rna for the diagnosis of bacterial meningitis after neurosurgery |
| topic | Meningitis, Bacterial Craniotomy Cerebrospinal Fluid Diagnosis RNA, Ribosomal, 16S Sequence Analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322021000100220&tlng=en |
| work_keys_str_mv | AT laurovieiraperdigaoneto polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery AT michelimedeiros polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery AT suzetecleusaferreira polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery AT annashokonishiya polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery AT denisebrandaodeassis polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery AT icaroboszczowski polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery AT silviafigueiredocosta polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery AT annaslevin polymerasechainreactiontargeting16sribosomalrnaforthediagnosisofbacterialmeningitisafterneurosurgery |