Effects of storage conditions and digestion time on DNA amplification of biting midge (Culicoides) blood meals

Effects of storage conditions and digestion time on DNA amplification of biting midge (Culicoides) blood meals

Abstract Background Molecular analysis of blood meals is increasingly used to identify the hosts of biting insects such as midges and mosquitoes. Successful host identification depends on the availability of sufficient host DNA template for PCR amplification, making it important to understand how am...

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Bibliographic Details
Main Authors: Ben Bellekom, Abigail Bailey, Marion England, Zoe Langlands, Owen T. Lewis, Talya D. Hackett
Format: Article
Language:English
Published: BMC 2023-01-01
Series:Parasites & Vectors
Subjects:
Culicoides
DNA degradation
Metabarcoding
Blood meal
Biting diptera
DNA digestion
Online Access:https://doi.org/10.1186/s13071-022-05607-x

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