Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue
<p>Abstract</p> <p>The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2011-01-01
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| Series: | Nanoscale Research Letters |
| Online Access: | http://www.nanoscalereslett.com/content/6/1/386 |
| _version_ | 1797723182299348992 |
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| author | Aamand Jens Bache Michael Taboryski Rafael Schmid Silvan Jakobsen Mogens |
| author_facet | Aamand Jens Bache Michael Taboryski Rafael Schmid Silvan Jakobsen Mogens |
| author_sort | Aamand Jens |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4<sup>© </sup>system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4<sup>© </sup>system is a suited platform for a pesticide assay system.</p> |
| first_indexed | 2024-03-12T09:58:17Z |
| format | Article |
| id | doaj.art-3fce96032b1f4528b7e7ac595890c10f |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T09:58:17Z |
| publishDate | 2011-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-3fce96032b1f4528b7e7ac595890c10f2023-09-02T11:55:50ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2011-01-0161386Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residueAamand JensBache MichaelTaboryski RafaelSchmid SilvanJakobsen Mogens<p>Abstract</p> <p>The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4<sup>© </sup>system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4<sup>© </sup>system is a suited platform for a pesticide assay system.</p>http://www.nanoscalereslett.com/content/6/1/386 |
| spellingShingle | Aamand Jens Bache Michael Taboryski Rafael Schmid Silvan Jakobsen Mogens Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue Nanoscale Research Letters |
| title | Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue |
| title_full | Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue |
| title_fullStr | Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue |
| title_full_unstemmed | Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue |
| title_short | Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue |
| title_sort | investigations on antibody binding to a micro cantilever coated with a bam pesticide residue |
| url | http://www.nanoscalereslett.com/content/6/1/386 |
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