<b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental species
The research aimed to establish a protocol of micropropagation of Syngonanthus elegans. Seed germination in WPM media containing 0%, 25%, 50%, 75% and 100% salt concentrations was evaluated. Establishment of plantlets in WPM and MS media, with 50% or 100% salt concentrations, was studied. The additi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Eduem (Editora da Universidade Estadual de Maringá)
2014-07-01
|
| Series: | Acta Scientiarum: Agronomy |
| Subjects: | |
| Online Access: | http://186.233.154.254/ojs/index.php/ActaSciAgron/article/view/17946 |
| _version_ | 1817982012073443328 |
|---|---|
| author | Rogério Gomes Pêgo Patrícia Duarte de Oliveira Paiva Renato Paiva |
| author_facet | Rogério Gomes Pêgo Patrícia Duarte de Oliveira Paiva Renato Paiva |
| author_sort | Rogério Gomes Pêgo |
| collection | DOAJ |
| description | The research aimed to establish a protocol of micropropagation of Syngonanthus elegans. Seed germination in WPM media containing 0%, 25%, 50%, 75% and 100% salt concentrations was evaluated. Establishment of plantlets in WPM and MS media, with 50% or 100% salt concentrations, was studied. The addition of 0, 5, 10, 15, 20, 25 and 30 g L-1of sucrose in WPM media was evaluated. The effects of TDZ (0.0; 0.5; 1.0; 2.0 and 4.0 mg L-1) and ANA (0.0; 0.5 and 1.0 mg L-1) in all possible combinations were investigated. To test acclimatization, sand, PlantmaxTM or vermiculite substrates were evaluated by direct acclimatization and pre-acclimatization. The germination of S. elegans seeds was not influenced by salt concentration in the culture media, but the germination speed was affected. The WPM medium with 17 g L-1 of sucrose is recommended for establishment of plantlets. Callus formation occurs in presence of 0.5 or 1.0 mg L-1 of NAA, in absence of TDZ, or when same concentrations of NAA and TDZ were added. Sprouts were most frequently produced when 0.5+0.5 mg L-1 or 1.0+1.0 mg L-1 of NAA and TDZ were used. The S. elegans plants had survival rate was 25.6% after acclimatization. |
| first_indexed | 2024-04-13T23:13:53Z |
| format | Article |
| id | doaj.art-3fcf3e8761874e98af6b6575956d3cbb |
| institution | Directory Open Access Journal |
| issn | 1679-9275 1807-8621 |
| language | English |
| last_indexed | 2024-04-13T23:13:53Z |
| publishDate | 2014-07-01 |
| publisher | Eduem (Editora da Universidade Estadual de Maringá) |
| record_format | Article |
| series | Acta Scientiarum: Agronomy |
| spelling | doaj.art-3fcf3e8761874e98af6b6575956d3cbb2022-12-22T02:25:27ZengEduem (Editora da Universidade Estadual de Maringá)Acta Scientiarum: Agronomy1679-92751807-86212014-07-0136334735310.4025/actasciagron.v36i3.1794610793<b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental speciesRogério Gomes Pêgo0Patrícia Duarte de Oliveira Paiva1Renato Paiva2Universidade Federal de ViçosaUniversidade Federal de LavrasUniversidade Federal de LavrasThe research aimed to establish a protocol of micropropagation of Syngonanthus elegans. Seed germination in WPM media containing 0%, 25%, 50%, 75% and 100% salt concentrations was evaluated. Establishment of plantlets in WPM and MS media, with 50% or 100% salt concentrations, was studied. The addition of 0, 5, 10, 15, 20, 25 and 30 g L-1of sucrose in WPM media was evaluated. The effects of TDZ (0.0; 0.5; 1.0; 2.0 and 4.0 mg L-1) and ANA (0.0; 0.5 and 1.0 mg L-1) in all possible combinations were investigated. To test acclimatization, sand, PlantmaxTM or vermiculite substrates were evaluated by direct acclimatization and pre-acclimatization. The germination of S. elegans seeds was not influenced by salt concentration in the culture media, but the germination speed was affected. The WPM medium with 17 g L-1 of sucrose is recommended for establishment of plantlets. Callus formation occurs in presence of 0.5 or 1.0 mg L-1 of NAA, in absence of TDZ, or when same concentrations of NAA and TDZ were added. Sprouts were most frequently produced when 0.5+0.5 mg L-1 or 1.0+1.0 mg L-1 of NAA and TDZ were used. The S. elegans plants had survival rate was 25.6% after acclimatization.http://186.233.154.254/ojs/index.php/ActaSciAgron/article/view/17946floriculturein vitro cultivationmultiplicationacclimatization |
| spellingShingle | Rogério Gomes Pêgo Patrícia Duarte de Oliveira Paiva Renato Paiva <b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental species Acta Scientiarum: Agronomy floriculture in vitro cultivation multiplication acclimatization |
| title | <b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental species |
| title_full | <b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental species |
| title_fullStr | <b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental species |
| title_full_unstemmed | <b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental species |
| title_short | <b>Micropropagation protocol for <i>Syngonanthus elegans</i> (Bong.) Ruhland: an ornamental species |
| title_sort | b micropropagation protocol for i syngonanthus elegans i bong ruhland an ornamental species |
| topic | floriculture in vitro cultivation multiplication acclimatization |
| url | http://186.233.154.254/ojs/index.php/ActaSciAgron/article/view/17946 |
| work_keys_str_mv | AT rogeriogomespego bmicropropagationprotocolforisyngonanthuselegansibongruhlandanornamentalspecies AT patriciaduartedeoliveirapaiva bmicropropagationprotocolforisyngonanthuselegansibongruhlandanornamentalspecies AT renatopaiva bmicropropagationprotocolforisyngonanthuselegansibongruhlandanornamentalspecies |