<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway
<i>Cucumis sativus</i> L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a <i>C. sativus</i> seeds extrac...
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MDPI AG
2021-11-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/14/11/1197 |
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| author | Muqeet Wahid Fatima Saqib Hanadi Talal Ahmedah Claudia Mihaela Gavris Vincenzo De Feo Mircea Hogea Marius Moga Radu Chicea |
| author_facet | Muqeet Wahid Fatima Saqib Hanadi Talal Ahmedah Claudia Mihaela Gavris Vincenzo De Feo Mircea Hogea Marius Moga Radu Chicea |
| author_sort | Muqeet Wahid |
| collection | DOAJ |
| description | <i>Cucumis sativus</i> L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a <i>C. sativus</i> seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. <i>C. sativus</i> seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K<sup>+</sup> (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, <i>C. sativus</i> seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway. |
| first_indexed | 2024-03-10T05:09:48Z |
| format | Article |
| id | doaj.art-3fd0f28cb90d4402a06f80387ca6c1ab |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T05:09:48Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-3fd0f28cb90d4402a06f80387ca6c1ab2023-11-23T00:56:45ZengMDPI AGPharmaceuticals1424-82472021-11-011411119710.3390/ph14111197<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling PathwayMuqeet Wahid0Fatima Saqib1Hanadi Talal Ahmedah2Claudia Mihaela Gavris3Vincenzo De Feo4Mircea Hogea5Marius Moga6Radu Chicea7Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Rabigh 25732, Saudi ArabiaFaculty of Medicine, Transilvania University of Brasov, 500036 Brasov, RomaniaDepartment of Pharmacy, Salerno University, Fisciano, 84084 Salerno, ItalyFaculty of Medicine, Transilvania University of Brasov, 500036 Brasov, RomaniaFaculty of Medicine, Transilvania University of Brasov, 500036 Brasov, RomaniaFaculty of Medicine, Lucian Blaga University, 550024 Sibiu, Romania<i>Cucumis sativus</i> L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a <i>C. sativus</i> seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. <i>C. sativus</i> seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K<sup>+</sup> (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, <i>C. sativus</i> seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway.https://www.mdpi.com/1424-8247/14/11/1197asthmaantidiarrhealbronchodilator<i>Cucumis sativus</i>cucumberHPLC |
| spellingShingle | Muqeet Wahid Fatima Saqib Hanadi Talal Ahmedah Claudia Mihaela Gavris Vincenzo De Feo Mircea Hogea Marius Moga Radu Chicea <i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway Pharmaceuticals asthma antidiarrheal bronchodilator <i>Cucumis sativus</i> cucumber HPLC |
| title | <i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway |
| title_full | <i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway |
| title_fullStr | <i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway |
| title_full_unstemmed | <i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway |
| title_short | <i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway |
| title_sort | i cucumis sativus i l seeds ameliorate muscular spasm induced gastrointestinal and respiratory disorders by simultaneously inhibiting calcium mediated signaling pathway |
| topic | asthma antidiarrheal bronchodilator <i>Cucumis sativus</i> cucumber HPLC |
| url | https://www.mdpi.com/1424-8247/14/11/1197 |
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