The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell Lines
In women, breast cancer is the most commonly diagnosed cancer (11.7% of total cases) and the leading cause of cancer death (6.9%) worldwide. Bioactive dietary components such as <i>Sea buckthorn</i> berries are known for their high carotenoid content, which has been shown to possess anti...
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MDPI AG
2023-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/11/4486 |
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| author | Simona Visan Olga Soritau Corina Tatomir Oana Baldasici Loredana Balacescu Ovidiu Balacescu Patricia Muntean Cristina Gherasim Adela Pintea |
| author_facet | Simona Visan Olga Soritau Corina Tatomir Oana Baldasici Loredana Balacescu Ovidiu Balacescu Patricia Muntean Cristina Gherasim Adela Pintea |
| author_sort | Simona Visan |
| collection | DOAJ |
| description | In women, breast cancer is the most commonly diagnosed cancer (11.7% of total cases) and the leading cause of cancer death (6.9%) worldwide. Bioactive dietary components such as <i>Sea buckthorn</i> berries are known for their high carotenoid content, which has been shown to possess anti-cancer properties. Considering the limited number of studies investigating the bioactive properties of carotenoids in breast cancer, the aim of this study was to investigate the antiproliferative, antioxidant, and proapoptotic properties of saponified lipophilic <i>Sea buckthorn</i> berries extract (LSBE) in two breast cancer cell lines with different phenotypes: T47D (ER+, PR+, HER2−) and BT-549 (ER-, PR-, HER2−). The antiproliferative effects of LSBE were evaluated by an Alamar Blue assay, the extracellular antioxidant capacity was evaluated through DPPH, ABTS, and FRAP assays, the intracellular antioxidant capacity was evaluated through a DCFDA assay, and the apoptosis rate was assessed by flow cytometry. LSBE inhibited the proliferation of breast cancer cells in a concentration-dependent manner, with a mean IC<sub>50</sub> of 16 µM. LSBE has proven to be a good antioxidant both at the intracellular level, due to its ability to significantly decrease the ROS levels in both cell lines (<i>p</i> = 0.0279 for T47D, and <i>p</i> = 0.0188 for BT-549), and at the extracellular level, where the ABTS and DPPH inhibition vried between 3.38–56.8%, respectively 5.68–68.65%, and 35.6 mg/L equivalent ascorbic acid/g LSBE were recorded. Based on the results from the antioxidant assays, LSBE was found to have good antioxidant activity due to its rich carotenoid content. The flow cytometry results revealed that LSBE treatment induced significant alterations in late-stage apoptotic cells represented by 80.29% of T47D cells (<i>p</i> = 0.0119), and 40.6% of BT-549 cells (<i>p</i> = 0.0137). Considering the antiproliferative, antioxidant, and proapoptotic properties of the carotenoids from LSBE on breast cancer cells, further studies should investigate whether these bioactive dietary compounds could be used as nutraceuticals in breast cancer therapy. |
| first_indexed | 2024-03-11T03:00:31Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T03:00:31Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-3fd1259a39944e298044919e4f4c55652023-11-18T08:17:16ZengMDPI AGMolecules1420-30492023-06-012811448610.3390/molecules28114486The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell LinesSimona Visan0Olga Soritau1Corina Tatomir2Oana Baldasici3Loredana Balacescu4Ovidiu Balacescu5Patricia Muntean6Cristina Gherasim7Adela Pintea8Department of Genetics, Genomics, and Experimental Pathology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 400015 Cluj-Napoca, RomaniaDepartment of Cell Biology and Radiobiology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 400015 Cluj-Napoca, RomaniaDepartment of Cell Biology and Radiobiology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 400015 Cluj-Napoca, RomaniaDepartment of Genetics, Genomics, and Experimental Pathology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 400015 Cluj-Napoca, RomaniaDepartment of Genetics, Genomics, and Experimental Pathology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 400015 Cluj-Napoca, RomaniaDepartment of Genetics, Genomics, and Experimental Pathology, The Oncology Institute “Prof. Dr. Ion Chiricuta”, 400015 Cluj-Napoca, RomaniaDepartment of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaIn women, breast cancer is the most commonly diagnosed cancer (11.7% of total cases) and the leading cause of cancer death (6.9%) worldwide. Bioactive dietary components such as <i>Sea buckthorn</i> berries are known for their high carotenoid content, which has been shown to possess anti-cancer properties. Considering the limited number of studies investigating the bioactive properties of carotenoids in breast cancer, the aim of this study was to investigate the antiproliferative, antioxidant, and proapoptotic properties of saponified lipophilic <i>Sea buckthorn</i> berries extract (LSBE) in two breast cancer cell lines with different phenotypes: T47D (ER+, PR+, HER2−) and BT-549 (ER-, PR-, HER2−). The antiproliferative effects of LSBE were evaluated by an Alamar Blue assay, the extracellular antioxidant capacity was evaluated through DPPH, ABTS, and FRAP assays, the intracellular antioxidant capacity was evaluated through a DCFDA assay, and the apoptosis rate was assessed by flow cytometry. LSBE inhibited the proliferation of breast cancer cells in a concentration-dependent manner, with a mean IC<sub>50</sub> of 16 µM. LSBE has proven to be a good antioxidant both at the intracellular level, due to its ability to significantly decrease the ROS levels in both cell lines (<i>p</i> = 0.0279 for T47D, and <i>p</i> = 0.0188 for BT-549), and at the extracellular level, where the ABTS and DPPH inhibition vried between 3.38–56.8%, respectively 5.68–68.65%, and 35.6 mg/L equivalent ascorbic acid/g LSBE were recorded. Based on the results from the antioxidant assays, LSBE was found to have good antioxidant activity due to its rich carotenoid content. The flow cytometry results revealed that LSBE treatment induced significant alterations in late-stage apoptotic cells represented by 80.29% of T47D cells (<i>p</i> = 0.0119), and 40.6% of BT-549 cells (<i>p</i> = 0.0137). Considering the antiproliferative, antioxidant, and proapoptotic properties of the carotenoids from LSBE on breast cancer cells, further studies should investigate whether these bioactive dietary compounds could be used as nutraceuticals in breast cancer therapy.https://www.mdpi.com/1420-3049/28/11/4486<i>Sea buckthorn</i>carotenoidscytotoxicityapoptosisbreast cancerantioxidants |
| spellingShingle | Simona Visan Olga Soritau Corina Tatomir Oana Baldasici Loredana Balacescu Ovidiu Balacescu Patricia Muntean Cristina Gherasim Adela Pintea The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell Lines Molecules <i>Sea buckthorn</i> carotenoids cytotoxicity apoptosis breast cancer antioxidants |
| title | The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell Lines |
| title_full | The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell Lines |
| title_fullStr | The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell Lines |
| title_full_unstemmed | The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell Lines |
| title_short | The Bioactive Properties of Carotenoids from Lipophilic <i>Sea buckthorn</i> Extract (<i>Hippophae rhamnoides</i> L.) in Breast Cancer Cell Lines |
| title_sort | bioactive properties of carotenoids from lipophilic i sea buckthorn i extract i hippophae rhamnoides i l in breast cancer cell lines |
| topic | <i>Sea buckthorn</i> carotenoids cytotoxicity apoptosis breast cancer antioxidants |
| url | https://www.mdpi.com/1420-3049/28/11/4486 |
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