Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic Acid
The aim of this study was the development and optimization of some topical collagen-dextran sponges with flufenamic acid, designed to be potential dressings for burn wounds healing. The sponges were obtained by lyophilization of hydrogels based on type I fibrillar collagen gel extracted from calf hi...
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MDPI AG
2017-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/9/1552 |
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| author | Mihaela Violeta Ghica Mădălina Georgiana Albu Kaya Cristina-Elena Dinu-Pîrvu Dumitru Lupuleasa Denisa Ioana Udeanu |
| author_facet | Mihaela Violeta Ghica Mădălina Georgiana Albu Kaya Cristina-Elena Dinu-Pîrvu Dumitru Lupuleasa Denisa Ioana Udeanu |
| author_sort | Mihaela Violeta Ghica |
| collection | DOAJ |
| description | The aim of this study was the development and optimization of some topical collagen-dextran sponges with flufenamic acid, designed to be potential dressings for burn wounds healing. The sponges were obtained by lyophilization of hydrogels based on type I fibrillar collagen gel extracted from calf hide, dextran and flufenamic acid, crosslinked and un-crosslinked, and designed according to a 3-factor, 3-level Box-Behnken experimental design. The sponges showed good fluid uptake ability quantified by a high swelling ratio. The flufenamic acid release profiles from sponges presented two stages—burst effect resulting in a rapid inflammation reduction, and gradual delivery ensuring the anti-inflammatory effect over a longer burn healing period. The resistance to enzymatic degradation was monitored through a weight loss parameter. The optimization of the sponge formulations was performed based on an experimental design technique combined with response surface methodology, followed by the Taguchi approach to select those formulations that are the least affected by the noise factors. The treatment of experimentally induced burns on animals with selected sponges accelerated the wound healing process and promoted a faster regeneration of the affected epithelial tissues compared to the control group. The results generated by the complex sponge characterization indicate that these formulations could be successfully used for burn dressing applications. |
| first_indexed | 2024-04-13T14:38:42Z |
| format | Article |
| id | doaj.art-3fd198c7615843108c74ccc5aa0e6684 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T14:38:42Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3fd198c7615843108c74ccc5aa0e66842022-12-22T02:42:58ZengMDPI AGMolecules1420-30492017-09-01229155210.3390/molecules22091552molecules22091552Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic AcidMihaela Violeta Ghica0Mădălina Georgiana Albu Kaya1Cristina-Elena Dinu-Pîrvu2Dumitru Lupuleasa3Denisa Ioana Udeanu4Department of Physical and Colloidal Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy ”Carol Davila”, Bucharest 020956, RomaniaDepartment of Collagen Research, Division of Leather and Footwear Research Institute, National Research & Development Institute for Textiles and Leather, Bucharest 031215, RomaniaDepartment of Physical and Colloidal Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy ”Carol Davila”, Bucharest 020956, RomaniaDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy ”Carol Davila”, Bucharest 020956, RomaniaDepartment of Clinical Laboratory and Food Safety, Faculty of Pharmacy, University of Medicine and Pharmacy ”Carol Davila”, Bucharest 020956, RomaniaThe aim of this study was the development and optimization of some topical collagen-dextran sponges with flufenamic acid, designed to be potential dressings for burn wounds healing. The sponges were obtained by lyophilization of hydrogels based on type I fibrillar collagen gel extracted from calf hide, dextran and flufenamic acid, crosslinked and un-crosslinked, and designed according to a 3-factor, 3-level Box-Behnken experimental design. The sponges showed good fluid uptake ability quantified by a high swelling ratio. The flufenamic acid release profiles from sponges presented two stages—burst effect resulting in a rapid inflammation reduction, and gradual delivery ensuring the anti-inflammatory effect over a longer burn healing period. The resistance to enzymatic degradation was monitored through a weight loss parameter. The optimization of the sponge formulations was performed based on an experimental design technique combined with response surface methodology, followed by the Taguchi approach to select those formulations that are the least affected by the noise factors. The treatment of experimentally induced burns on animals with selected sponges accelerated the wound healing process and promoted a faster regeneration of the affected epithelial tissues compared to the control group. The results generated by the complex sponge characterization indicate that these formulations could be successfully used for burn dressing applications.https://www.mdpi.com/1420-3049/22/9/1552collagen spongesflufenamic acidin vitro drug deliveryexperimental designtaguchi techniquein vivo burn healing |
| spellingShingle | Mihaela Violeta Ghica Mădălina Georgiana Albu Kaya Cristina-Elena Dinu-Pîrvu Dumitru Lupuleasa Denisa Ioana Udeanu Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic Acid Molecules collagen sponges flufenamic acid in vitro drug delivery experimental design taguchi technique in vivo burn healing |
| title | Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic Acid |
| title_full | Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic Acid |
| title_fullStr | Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic Acid |
| title_full_unstemmed | Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic Acid |
| title_short | Development, Optimization and In Vitro/In Vivo Characterization of Collagen-Dextran Spongious Wound Dressings Loaded with Flufenamic Acid |
| title_sort | development optimization and in vitro in vivo characterization of collagen dextran spongious wound dressings loaded with flufenamic acid |
| topic | collagen sponges flufenamic acid in vitro drug delivery experimental design taguchi technique in vivo burn healing |
| url | https://www.mdpi.com/1420-3049/22/9/1552 |
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