The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development
The evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, the Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polym...
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PeerJ Inc.
2017-06-01
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| author | Markéta Kostrouchová David Kostrouch Ahmed A. Chughtai Filip Kaššák Jan P. Novotný Veronika Kostrouchová Aleš Benda Michael W. Krause Vladimír Saudek Marta Kostrouchová Zdeněk Kostrouch |
| author_facet | Markéta Kostrouchová David Kostrouch Ahmed A. Chughtai Filip Kaššák Jan P. Novotný Veronika Kostrouchová Aleš Benda Michael W. Krause Vladimír Saudek Marta Kostrouchová Zdeněk Kostrouch |
| author_sort | Markéta Kostrouchová |
| collection | DOAJ |
| description | The evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, the Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polymerase II activity. One Mediator subunit, MED28, is known to interact with cytoplasmic structural proteins, providing a potential direct link between cytoplasmic dynamics and the control of gene transcription. Although identified in many animals and plants, MED28 is not present in yeast; no bona fide MED28 has been described previously in Caenorhabditis elegans. Here, we identify bioinformatically F28F8.5, an uncharacterized predicted protein, as the nematode homologue of MED28. As in other Metazoa, F28F8.5 has dual nuclear and cytoplasmic localization and plays critical roles in the regulation of development. F28F8.5 is a vital gene and its null mutants have severely malformed gonads and do not reproduce. F28F8.5 interacts on the protein level with the Mediator subunits MDT-6 and MDT-30. Our results indicate that F28F8.5 is an orthologue of MED28 and suggest that the potential to link cytoplasmic and nuclear events is conserved between MED28 vertebrate and nematode orthologues. |
| first_indexed | 2024-03-09T07:27:01Z |
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| id | doaj.art-3fd1b7a6270249538fa62b81c1e9027b |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:27:01Z |
| publishDate | 2017-06-01 |
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| spelling | doaj.art-3fd1b7a6270249538fa62b81c1e9027b2023-12-03T06:52:20ZengPeerJ Inc.PeerJ2167-83592017-06-015e339010.7717/peerj.3390The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans developmentMarkéta Kostrouchová0David Kostrouch1Ahmed A. Chughtai2Filip Kaššák3Jan P. Novotný4Veronika Kostrouchová5Aleš Benda6Michael W. Krause7Vladimír Saudek8Marta Kostrouchová9Zdeněk Kostrouch10Biocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicBiocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicBiocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicBiocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicBiocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicBiocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicImaging Methods Core Facility, BIOCEV, Faculty of Science, Charles University, Prague, Czech RepublicLaboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USAMetabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge, Cambridge, UKBiocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicBiocev, First Faculty of Medicine, Charles University, Prague, Czech RepublicThe evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, the Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polymerase II activity. One Mediator subunit, MED28, is known to interact with cytoplasmic structural proteins, providing a potential direct link between cytoplasmic dynamics and the control of gene transcription. Although identified in many animals and plants, MED28 is not present in yeast; no bona fide MED28 has been described previously in Caenorhabditis elegans. Here, we identify bioinformatically F28F8.5, an uncharacterized predicted protein, as the nematode homologue of MED28. As in other Metazoa, F28F8.5 has dual nuclear and cytoplasmic localization and plays critical roles in the regulation of development. F28F8.5 is a vital gene and its null mutants have severely malformed gonads and do not reproduce. F28F8.5 interacts on the protein level with the Mediator subunits MDT-6 and MDT-30. Our results indicate that F28F8.5 is an orthologue of MED28 and suggest that the potential to link cytoplasmic and nuclear events is conserved between MED28 vertebrate and nematode orthologues.https://peerj.com/articles/3390.pdfGene expression regulationCaenorhabditis elegansDevelopmentEvolutionMediator complexMED28 |
| spellingShingle | Markéta Kostrouchová David Kostrouch Ahmed A. Chughtai Filip Kaššák Jan P. Novotný Veronika Kostrouchová Aleš Benda Michael W. Krause Vladimír Saudek Marta Kostrouchová Zdeněk Kostrouch The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development PeerJ Gene expression regulation Caenorhabditis elegans Development Evolution Mediator complex MED28 |
| title | The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development |
| title_full | The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development |
| title_fullStr | The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development |
| title_full_unstemmed | The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development |
| title_short | The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development |
| title_sort | nematode homologue of mediator complex subunit 28 f28f8 5 is a critical regulator of c elegans development |
| topic | Gene expression regulation Caenorhabditis elegans Development Evolution Mediator complex MED28 |
| url | https://peerj.com/articles/3390.pdf |
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