Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i>
The Colorado potato beetle <i>Leptinotarsa decemlineata</i> is an insect pest that threatens potato crops globally. The primary method to control its damage on potato plants is the use of insecticides, including imidacloprid, chlorantraniliprole and spinosad. However, insecticide resista...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/11/820 |
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| author | Pierre Bastarache Gabriel Wajnberg Pascal Dumas Simi Chacko Jacynthe Lacroix Nicolas Crapoulet Chandra E. Moffat Pier Morin |
| author_facet | Pierre Bastarache Gabriel Wajnberg Pascal Dumas Simi Chacko Jacynthe Lacroix Nicolas Crapoulet Chandra E. Moffat Pier Morin |
| author_sort | Pierre Bastarache |
| collection | DOAJ |
| description | The Colorado potato beetle <i>Leptinotarsa decemlineata</i> is an insect pest that threatens potato crops globally. The primary method to control its damage on potato plants is the use of insecticides, including imidacloprid, chlorantraniliprole and spinosad. However, insecticide resistance has been frequently observed in Colorado potato beetles. The molecular targets and the basis of resistance to imidacloprid and chlorantraniliprole have both been previously quantified. This work was undertaken with the overarching goal of better characterizing the molecular changes associated with spinosad exposure in this insect pest. Next-generation sequencing was conducted to identify transcripts that were differentially expressed between Colorado potato beetles exposed to spinosad versus control insects. Results showed several transcripts that exhibit different expression levels between the two conditions, including ones coding for venom carboxylesterase-6, chitinase 10, juvenile hormone esterase and multidrug resistance-associated protein 4. In addition, several microRNAs, such as miR-12-3p and miR-750-3p, were also modulated in the investigated conditions. Overall, this work reveals a molecular footprint underlying spinosad response in Colorado potato beetles and provides novel leads that could be targeted as part of RNAi-based approaches to control this insect pest. |
| first_indexed | 2024-03-10T14:40:50Z |
| format | Article |
| id | doaj.art-3fd201f58255405f9a2184185f677358 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T14:40:50Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-3fd201f58255405f9a2184185f6773582023-11-20T21:48:12ZengMDPI AGInsects2075-44502020-11-01111182010.3390/insects11110820Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i>Pierre Bastarache0Gabriel Wajnberg1Pascal Dumas2Simi Chacko3Jacynthe Lacroix4Nicolas Crapoulet5Chandra E. Moffat6Pier Morin7Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, Moncton, NB E1A 3E9, CanadaAtlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, Moncton, NB E1C 8X3, CanadaDepartment of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, Moncton, NB E1A 3E9, CanadaAtlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, Moncton, NB E1C 8X3, CanadaAtlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, Moncton, NB E1C 8X3, CanadaAtlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, Moncton, NB E1C 8X3, CanadaFredericton Research and Development Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, Fredericton, NB E3B 4Z7, CanadaDepartment of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, Moncton, NB E1A 3E9, CanadaThe Colorado potato beetle <i>Leptinotarsa decemlineata</i> is an insect pest that threatens potato crops globally. The primary method to control its damage on potato plants is the use of insecticides, including imidacloprid, chlorantraniliprole and spinosad. However, insecticide resistance has been frequently observed in Colorado potato beetles. The molecular targets and the basis of resistance to imidacloprid and chlorantraniliprole have both been previously quantified. This work was undertaken with the overarching goal of better characterizing the molecular changes associated with spinosad exposure in this insect pest. Next-generation sequencing was conducted to identify transcripts that were differentially expressed between Colorado potato beetles exposed to spinosad versus control insects. Results showed several transcripts that exhibit different expression levels between the two conditions, including ones coding for venom carboxylesterase-6, chitinase 10, juvenile hormone esterase and multidrug resistance-associated protein 4. In addition, several microRNAs, such as miR-12-3p and miR-750-3p, were also modulated in the investigated conditions. Overall, this work reveals a molecular footprint underlying spinosad response in Colorado potato beetles and provides novel leads that could be targeted as part of RNAi-based approaches to control this insect pest.https://www.mdpi.com/2075-4450/11/11/820Colorado potato beetleinsecticidesmicroRNAsnext-generation sequencingnon-coding RNAsspinosad |
| spellingShingle | Pierre Bastarache Gabriel Wajnberg Pascal Dumas Simi Chacko Jacynthe Lacroix Nicolas Crapoulet Chandra E. Moffat Pier Morin Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i> Insects Colorado potato beetle insecticides microRNAs next-generation sequencing non-coding RNAs spinosad |
| title | Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i> |
| title_full | Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i> |
| title_fullStr | Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i> |
| title_full_unstemmed | Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i> |
| title_short | Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle, <i>Leptinotarsa decemlineata</i> |
| title_sort | transcriptomics based approach identifies spinosad associated targets in the colorado potato beetle i leptinotarsa decemlineata i |
| topic | Colorado potato beetle insecticides microRNAs next-generation sequencing non-coding RNAs spinosad |
| url | https://www.mdpi.com/2075-4450/11/11/820 |
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