Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in China
The Cat Que orthobunyavirus has been found in mosquitoes, birds, pigs, and humans, suggesting its wide range of hosts and potential public health implications. During arbovirus surveillance in 2013, the HN1304M virus was isolated from naturally occurring <i>Culicoides</i> biting midges i...
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MDPI AG
2022-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/9/1049 |
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| author | Ziqian Xu Lei Cao Liang Cai Shihong Fu Kai Nie Qikai Yin Yuxi Cao Guoping Liu Yunzhi Liu Hong Zhang Lidong Gao Ying He Huanyu Wang Guodong Liang |
| author_facet | Ziqian Xu Lei Cao Liang Cai Shihong Fu Kai Nie Qikai Yin Yuxi Cao Guoping Liu Yunzhi Liu Hong Zhang Lidong Gao Ying He Huanyu Wang Guodong Liang |
| author_sort | Ziqian Xu |
| collection | DOAJ |
| description | The Cat Que orthobunyavirus has been found in mosquitoes, birds, pigs, and humans, suggesting its wide range of hosts and potential public health implications. During arbovirus surveillance in 2013, the HN1304M virus was isolated from naturally occurring <i>Culicoides</i> biting midges in Hunan Province, southern China. The virus was cytopathic to BHK-21 cells and showed stable passage, but was not cytopathic to C6/36 cells. Determination and analysis of the viral genome sequence revealed that HN1304M is an RNA virus with three gene segments, namely, L, M, and S. The nucleotide and amino acid sequence homologies of HN1304M to Cat Que viruses in the Manzanilla species complex were 90.3–99.4%, and 95–100%, respectively, while the homologies to other viruses in this species complex were 74–86.6% and 78.1–96.1%, respectively. A phylogenetic analysis of the viral genes revealed that HN1304M formed an evolutionary branch with other Cat Que viruses isolated from mosquitoes, pigs, birds, and humans, which was completely independent of the other viruses in this complex. The fact that the Cat Que virus was isolated from <i>Culicoides</i> suggests that biting midges may participate in the natural circulation of Cat Que viruses. |
| first_indexed | 2024-03-09T22:53:04Z |
| format | Article |
| id | doaj.art-3fd565d9dd904d808578ba3c2b793d01 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:53:04Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-3fd565d9dd904d808578ba3c2b793d012023-11-23T18:16:34ZengMDPI AGPathogens2076-08172022-09-01119104910.3390/pathogens11091049Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in ChinaZiqian Xu0Lei Cao1Liang Cai2Shihong Fu3Kai Nie4Qikai Yin5Yuxi Cao6Guoping Liu7Yunzhi Liu8Hong Zhang9Lidong Gao10Ying He11Huanyu Wang12Guodong Liang13State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaHunan Center for Disease Control and Prevention, Changsha 410005, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaDepartment of Pest Control, Center for Disease Control and Prevention of Northern Theater Command, Shenyang 110034, ChinaFoshan Blood Center, Foshan 528000, ChinaHunan Center for Disease Control and Prevention, Changsha 410005, ChinaHunan Center for Disease Control and Prevention, Changsha 410005, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaThe Cat Que orthobunyavirus has been found in mosquitoes, birds, pigs, and humans, suggesting its wide range of hosts and potential public health implications. During arbovirus surveillance in 2013, the HN1304M virus was isolated from naturally occurring <i>Culicoides</i> biting midges in Hunan Province, southern China. The virus was cytopathic to BHK-21 cells and showed stable passage, but was not cytopathic to C6/36 cells. Determination and analysis of the viral genome sequence revealed that HN1304M is an RNA virus with three gene segments, namely, L, M, and S. The nucleotide and amino acid sequence homologies of HN1304M to Cat Que viruses in the Manzanilla species complex were 90.3–99.4%, and 95–100%, respectively, while the homologies to other viruses in this species complex were 74–86.6% and 78.1–96.1%, respectively. A phylogenetic analysis of the viral genes revealed that HN1304M formed an evolutionary branch with other Cat Que viruses isolated from mosquitoes, pigs, birds, and humans, which was completely independent of the other viruses in this complex. The fact that the Cat Que virus was isolated from <i>Culicoides</i> suggests that biting midges may participate in the natural circulation of Cat Que viruses.https://www.mdpi.com/2076-0817/11/9/1049orthobunyavirusManzanilla species complexCat Que virus<i>Culicoides</i>arbovirus |
| spellingShingle | Ziqian Xu Lei Cao Liang Cai Shihong Fu Kai Nie Qikai Yin Yuxi Cao Guoping Liu Yunzhi Liu Hong Zhang Lidong Gao Ying He Huanyu Wang Guodong Liang Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in China Pathogens orthobunyavirus Manzanilla species complex Cat Que virus <i>Culicoides</i> arbovirus |
| title | Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in China |
| title_full | Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in China |
| title_fullStr | Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in China |
| title_full_unstemmed | Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in China |
| title_short | Molecular Characterization of HN1304M, a Cat Que Virus Isolated from Midges in China |
| title_sort | molecular characterization of hn1304m a cat que virus isolated from midges in china |
| topic | orthobunyavirus Manzanilla species complex Cat Que virus <i>Culicoides</i> arbovirus |
| url | https://www.mdpi.com/2076-0817/11/9/1049 |
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