Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease
Objective: To analyze the percentage of collagen fibers and mast cell density in the left ventricular myocardium of autopsied patients with and without hypertensive heart disease. Methods: Thirty fragments of left ventricular myocardium were obtained from individuals autopsied at the Clinical Hospit...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-02-01
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| Series: | Revista Portuguesa de Cardiologia |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0870255120300810 |
| _version_ | 1818485766512181248 |
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| author | Guilherme Ribeiro Juliano Mariana Fleury Skaf Luciana Santos Ramalho Gabriela Ribeiro Juliano Bianca Gonçalves Silva Torquato Mariana Silva Oliveira Flávia Aparecida Oliveira Ana Paula Espíndula Camila Lourencini Cavellani Vicente de Paula Antunes Teixeira Mara Lúcia da Fonseca Ferraz |
| author_facet | Guilherme Ribeiro Juliano Mariana Fleury Skaf Luciana Santos Ramalho Gabriela Ribeiro Juliano Bianca Gonçalves Silva Torquato Mariana Silva Oliveira Flávia Aparecida Oliveira Ana Paula Espíndula Camila Lourencini Cavellani Vicente de Paula Antunes Teixeira Mara Lúcia da Fonseca Ferraz |
| author_sort | Guilherme Ribeiro Juliano |
| collection | DOAJ |
| description | Objective: To analyze the percentage of collagen fibers and mast cell density in the left ventricular myocardium of autopsied patients with and without hypertensive heart disease. Methods: Thirty fragments of left ventricular myocardium were obtained from individuals autopsied at the Clinical Hospital of the Federal University of Triângulo Mineiro (UFTM) in the period from 1987 to 2017. Individuals were divided into two groups: those with hypertensive heart disease (HD) and those with no heart disease (ND). Subjects were also assessed according to age, gender and race (white and non-white). Collagen fibers were quantified by computed morphometry and mast cell density was assessed by immunohistochemical methods. Results: There were significantly more collagen fibers in the left ventricle in the HD group than in the ND group (p< 0.001). Mast cell density was significantly higher in the left ventricle of individuals with HD immunolabeled with anti-chymase and anti-tryptase antibodies (p=0.02) and also of those immunolabeled only with anti-tryptase antibodies (p=0.03). Analyzing the HD group, there was a significant positive correlation between the percentage of collagen fibers in the left ventricle and mast cell density immunolabeled by anti-chymase and anti-tryptase antibodies (p=0.04) and also mast cell density immunolabeled only with anti-tryptase antibodies (p=0.02). Conclusions: Mast cells are involved in the development of hypertensive heart disease, contributing to the remodeling of collagen fibers in this disease. Resumo: Objetivo: Analisar a porcentagem de fibras colágenas e a densidade mastocitária no miocárdio ventricular esquerdo de pacientes autopsiados com e sem cardiopatia hipertensiva. Métodos: Foram obtidos 30 fragmentos do miocárdio ventricular esquerdo de indivíduos autopsiados no Hospital Clínico da Universidade Federal do Triângulo Mineiro (UFTM) em 1987-2017, agrupados em indivíduos com cardiopatia hipertensiva (HD) e sem cardiopatia (ND) e avaliados de acordo com fatores como idade, sexo (masculino e feminino) e cor (branco e não branco). A quantificação das fibras colágenas foi feita por morfometria computadorizada e a densidade mastocitária foi avaliada por métodos imuno-histoquímicos. Resultados: Houve aumento significativo das fibras colágenas no ventrículo esquerdo no grupo HD em comparação com o grupo ND (p < 0,001). Quanto à densidade mastocitária, houve aumento significativo no ventrículo esquerdo de indivíduos com HD imunomarcados por antiquimase/antitriptase (p=0,02) e também na densidade mastocitária no ventrículo esquerdo de indivíduos imunomarcados por antitriptase (p=0,03). Analisando o grupo de HD, houve uma correlação positiva e significativa entre a porcentagem de fibras colágenas no ventrículo esquerdo e a densidade mastocitária no ventrículo esquerdo imunomarcados por antiquimase/antitriptase (p=0,04) e também na densidade mastocitária no ventrículo esquerdo de indivíduos imunomarcados por antitriptase (p=0,02). Conclusões: Os mastócitos estão envolvidos no desenvolvimento da cardiopatia hipertensiva, contribuem para a remodelação das fibras colágenas nessa doença. |
| first_indexed | 2024-12-10T16:13:17Z |
| format | Article |
| id | doaj.art-3fd6ce0691b846f198e3a9bcb99f40fd |
| institution | Directory Open Access Journal |
| issn | 0870-2551 |
| language | English |
| last_indexed | 2024-12-10T16:13:17Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Revista Portuguesa de Cardiologia |
| spelling | doaj.art-3fd6ce0691b846f198e3a9bcb99f40fd2022-12-22T01:42:03ZengElsevierRevista Portuguesa de Cardiologia0870-25512020-02-013928996Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart diseaseGuilherme Ribeiro Juliano0Mariana Fleury Skaf1Luciana Santos Ramalho2Gabriela Ribeiro Juliano3Bianca Gonçalves Silva Torquato4Mariana Silva Oliveira5Flávia Aparecida Oliveira6Ana Paula Espíndula7Camila Lourencini Cavellani8Vicente de Paula Antunes Teixeira9Mara Lúcia da Fonseca Ferraz10General Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, Brazil; Corresponding author.General Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilInstitute of Tropical Pathology and Public Health (IPTSP), Federal University of Goiás (UFG), Goiânia, GO, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilGeneral Pathology Department, Biological and Natural Sciences Institute (ICBN), Federal University of Triângulo Mineiro (UFTM), Uberaba, MG, BrazilObjective: To analyze the percentage of collagen fibers and mast cell density in the left ventricular myocardium of autopsied patients with and without hypertensive heart disease. Methods: Thirty fragments of left ventricular myocardium were obtained from individuals autopsied at the Clinical Hospital of the Federal University of Triângulo Mineiro (UFTM) in the period from 1987 to 2017. Individuals were divided into two groups: those with hypertensive heart disease (HD) and those with no heart disease (ND). Subjects were also assessed according to age, gender and race (white and non-white). Collagen fibers were quantified by computed morphometry and mast cell density was assessed by immunohistochemical methods. Results: There were significantly more collagen fibers in the left ventricle in the HD group than in the ND group (p< 0.001). Mast cell density was significantly higher in the left ventricle of individuals with HD immunolabeled with anti-chymase and anti-tryptase antibodies (p=0.02) and also of those immunolabeled only with anti-tryptase antibodies (p=0.03). Analyzing the HD group, there was a significant positive correlation between the percentage of collagen fibers in the left ventricle and mast cell density immunolabeled by anti-chymase and anti-tryptase antibodies (p=0.04) and also mast cell density immunolabeled only with anti-tryptase antibodies (p=0.02). Conclusions: Mast cells are involved in the development of hypertensive heart disease, contributing to the remodeling of collagen fibers in this disease. Resumo: Objetivo: Analisar a porcentagem de fibras colágenas e a densidade mastocitária no miocárdio ventricular esquerdo de pacientes autopsiados com e sem cardiopatia hipertensiva. Métodos: Foram obtidos 30 fragmentos do miocárdio ventricular esquerdo de indivíduos autopsiados no Hospital Clínico da Universidade Federal do Triângulo Mineiro (UFTM) em 1987-2017, agrupados em indivíduos com cardiopatia hipertensiva (HD) e sem cardiopatia (ND) e avaliados de acordo com fatores como idade, sexo (masculino e feminino) e cor (branco e não branco). A quantificação das fibras colágenas foi feita por morfometria computadorizada e a densidade mastocitária foi avaliada por métodos imuno-histoquímicos. Resultados: Houve aumento significativo das fibras colágenas no ventrículo esquerdo no grupo HD em comparação com o grupo ND (p < 0,001). Quanto à densidade mastocitária, houve aumento significativo no ventrículo esquerdo de indivíduos com HD imunomarcados por antiquimase/antitriptase (p=0,02) e também na densidade mastocitária no ventrículo esquerdo de indivíduos imunomarcados por antitriptase (p=0,03). Analisando o grupo de HD, houve uma correlação positiva e significativa entre a porcentagem de fibras colágenas no ventrículo esquerdo e a densidade mastocitária no ventrículo esquerdo imunomarcados por antiquimase/antitriptase (p=0,04) e também na densidade mastocitária no ventrículo esquerdo de indivíduos imunomarcados por antitriptase (p=0,02). Conclusões: Os mastócitos estão envolvidos no desenvolvimento da cardiopatia hipertensiva, contribuem para a remodelação das fibras colágenas nessa doença.http://www.sciencedirect.com/science/article/pii/S0870255120300810AutopsiaCardiopatia hipertensivaMastócitos |
| spellingShingle | Guilherme Ribeiro Juliano Mariana Fleury Skaf Luciana Santos Ramalho Gabriela Ribeiro Juliano Bianca Gonçalves Silva Torquato Mariana Silva Oliveira Flávia Aparecida Oliveira Ana Paula Espíndula Camila Lourencini Cavellani Vicente de Paula Antunes Teixeira Mara Lúcia da Fonseca Ferraz Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease Revista Portuguesa de Cardiologia Autopsia Cardiopatia hipertensiva Mastócitos |
| title | Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease |
| title_full | Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease |
| title_fullStr | Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease |
| title_full_unstemmed | Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease |
| title_short | Analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease |
| title_sort | analysis of mast cells and myocardial fibrosis in autopsied patients with hypertensive heart disease |
| topic | Autopsia Cardiopatia hipertensiva Mastócitos |
| url | http://www.sciencedirect.com/science/article/pii/S0870255120300810 |
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