Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker
In 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins using the methods of the PAGE ISTA. On the basis...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2003-03-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200303-0001_detection-of-the-varietal-purity-in-sample-of-harvested-wheat-and-triticale-grains-by-prolamin-marker.php |
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| author | T. Vyhnánek J. Bednář |
| author_facet | T. Vyhnánek J. Bednář |
| author_sort | T. Vyhnánek |
| collection | DOAJ |
| description | In 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins using the methods of the PAGE ISTA. On the basis of the identity index two sister prolamin lines with different percentage of participation, which was based on the weather conditions of the year of harvest, were discovered in seven wheat genotypes (Astella, Brea, Hana, Ilona, Siria, Sofia and Šárka) and two triticale genotypes (Tornádo and KM 779). A foreign genotype was detected in the Hana and Astella varieties. The identity index of the impurity to the Astella and Hana variety (i.e. ii = 0.28 and ii = 0.20, respectively) was considerably lower. In an unknown genotype (impurity) we detected the gliadin block Gld 1B3, which is the genetic marker of rye translocation T1BL.1RS, the Sr31 gene of resistance to black rust, higher cold resistance and the marker of poor baking quality (presence of secalin genes). The results proved the potential practical application of the method of electrophoretic detection of polymorphism of prolamin proteins as markers of impurities of foreign genotypes in a seed sample. |
| first_indexed | 2024-04-10T08:13:57Z |
| format | Article |
| id | doaj.art-3fd7b424b63d4c1280814673cccd1fe5 |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:13:57Z |
| publishDate | 2003-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-3fd7b424b63d4c1280814673cccd1fe52023-02-23T03:44:52ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682003-03-01493959810.17221/4096-PSEpse-200303-0001Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin markerT. Vyhnánek0J. Bednář1Mendel University of Agriculture and Forestry in Brno, Czech RepublicMendel University of Agriculture and Forestry in Brno, Czech RepublicIn 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins using the methods of the PAGE ISTA. On the basis of the identity index two sister prolamin lines with different percentage of participation, which was based on the weather conditions of the year of harvest, were discovered in seven wheat genotypes (Astella, Brea, Hana, Ilona, Siria, Sofia and Šárka) and two triticale genotypes (Tornádo and KM 779). A foreign genotype was detected in the Hana and Astella varieties. The identity index of the impurity to the Astella and Hana variety (i.e. ii = 0.28 and ii = 0.20, respectively) was considerably lower. In an unknown genotype (impurity) we detected the gliadin block Gld 1B3, which is the genetic marker of rye translocation T1BL.1RS, the Sr31 gene of resistance to black rust, higher cold resistance and the marker of poor baking quality (presence of secalin genes). The results proved the potential practical application of the method of electrophoretic detection of polymorphism of prolamin proteins as markers of impurities of foreign genotypes in a seed sample.https://pse.agriculturejournals.cz/artkey/pse-200303-0001_detection-of-the-varietal-purity-in-sample-of-harvested-wheat-and-triticale-grains-by-prolamin-marker.phpwinter wheatwinter triticaleprolamin proteinselectrophoresisadmixture |
| spellingShingle | T. Vyhnánek J. Bednář Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker Plant, Soil and Environment winter wheat winter triticale prolamin proteins electrophoresis admixture |
| title | Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker |
| title_full | Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker |
| title_fullStr | Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker |
| title_full_unstemmed | Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker |
| title_short | Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker |
| title_sort | detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker |
| topic | winter wheat winter triticale prolamin proteins electrophoresis admixture |
| url | https://pse.agriculturejournals.cz/artkey/pse-200303-0001_detection-of-the-varietal-purity-in-sample-of-harvested-wheat-and-triticale-grains-by-prolamin-marker.php |
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