Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES study
Circulating miRNA may contribute to the development of adverse birth outcomes. However, few studies have investigated extracellular vesicle (EV) miRNA, which play important roles in intercellular communication, or compared miRNA at multiple time points in pregnancy. In the current study, 800 miRNA w...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-03-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2021.1899887 |
| _version_ | 1797678808462000128 |
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| author | Caitlin G Howe Helen B Foley Elizabeth M Kennedy Sandrah P Eckel Thomas a Chavez Dema Faham Brendan H Grubbs Laila Al-Marayati Deborah Lerner Shakira Suglia Theresa M Bastain Carmen J Marsit Carrie V Breton |
| author_facet | Caitlin G Howe Helen B Foley Elizabeth M Kennedy Sandrah P Eckel Thomas a Chavez Dema Faham Brendan H Grubbs Laila Al-Marayati Deborah Lerner Shakira Suglia Theresa M Bastain Carmen J Marsit Carrie V Breton |
| author_sort | Caitlin G Howe |
| collection | DOAJ |
| description | Circulating miRNA may contribute to the development of adverse birth outcomes. However, few studies have investigated extracellular vesicle (EV) miRNA, which play important roles in intercellular communication, or compared miRNA at multiple time points in pregnancy. In the current study, 800 miRNA were profiled for EVs from maternal plasma collected in early (median: 12.5 weeks) and late (median: 31.8 weeks) pregnancy from 156 participants in the MADRES Study, a health disparity pregnancy cohort. Associations between miRNA and birth weight, birth weight for gestational age (GA), and GA at birth were examined using covariate-adjusted robust linear regression. Differences by infant sex and maternal BMI were also investigated. Late pregnancy measures of 13 miRNA were associated with GA at birth (PFDR<0.050). Negative associations were observed for eight miRNA (miR-4454+ miR-7975, miR-4516, let-7b-5p, miR-126-3p, miR-29b-3p, miR-15a-5p, miR-15b-5p, miR-19b-3p) and positive associations for five miRNA (miR-212-3p, miR-584-5p, miR-608, miR-210-3p, miR-188-5p). Predicted target genes were enriched (PFDR<0.050) in pathways involved in organogenesis and placental development. An additional miRNA (miR-107), measured in late pregnancy, was positively associated with GA at birth in infants born to obese women (PFDR for BMI interaction = 0.011). In primary analyses, the associations between early pregnancy miRNA and birth outcomes were not statistically significant (PFDR≥0.05). However, sex-specific associations were observed for early pregnancy measures of 37 miRNA and GA at birth (PFDR for interactions<0.050). None of the miRNA were associated with fetal growth measures (PFDR≥0.050). Our findings suggest that EV miRNA in both early and late pregnancy may influence gestational duration. |
| first_indexed | 2024-03-11T23:05:18Z |
| format | Article |
| id | doaj.art-3fd851f09e66461bbd2e40757bc3f04d |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:05:18Z |
| publishDate | 2022-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-3fd851f09e66461bbd2e40757bc3f04d2023-09-21T13:09:25ZengTaylor & Francis GroupEpigenetics1559-22941559-23082022-03-0117326928510.1080/15592294.2021.18998871899887Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES studyCaitlin G Howe0Helen B Foley1Elizabeth M Kennedy2Sandrah P Eckel3Thomas a Chavez4Dema Faham5Brendan H Grubbs6Laila Al-Marayati7Deborah Lerner8Shakira Suglia9Theresa M Bastain10Carmen J Marsit11Carrie V Breton12Geisel School of Medicine at DartmouthKeck School of Medicine at the University of Southern CaliforniaEmory Rollins School of Public HealthKeck School of Medicine at the University of Southern CaliforniaKeck School of Medicine at the University of Southern CaliforniaKeck School of Medicine at the University of Southern CaliforniaKeck School of MedicineKeck School of MedicineEisner HealthEmory Rollins School of Public HealthKeck School of Medicine at the University of Southern CaliforniaEmory Rollins School of Public HealthKeck School of Medicine at the University of Southern CaliforniaCirculating miRNA may contribute to the development of adverse birth outcomes. However, few studies have investigated extracellular vesicle (EV) miRNA, which play important roles in intercellular communication, or compared miRNA at multiple time points in pregnancy. In the current study, 800 miRNA were profiled for EVs from maternal plasma collected in early (median: 12.5 weeks) and late (median: 31.8 weeks) pregnancy from 156 participants in the MADRES Study, a health disparity pregnancy cohort. Associations between miRNA and birth weight, birth weight for gestational age (GA), and GA at birth were examined using covariate-adjusted robust linear regression. Differences by infant sex and maternal BMI were also investigated. Late pregnancy measures of 13 miRNA were associated with GA at birth (PFDR<0.050). Negative associations were observed for eight miRNA (miR-4454+ miR-7975, miR-4516, let-7b-5p, miR-126-3p, miR-29b-3p, miR-15a-5p, miR-15b-5p, miR-19b-3p) and positive associations for five miRNA (miR-212-3p, miR-584-5p, miR-608, miR-210-3p, miR-188-5p). Predicted target genes were enriched (PFDR<0.050) in pathways involved in organogenesis and placental development. An additional miRNA (miR-107), measured in late pregnancy, was positively associated with GA at birth in infants born to obese women (PFDR for BMI interaction = 0.011). In primary analyses, the associations between early pregnancy miRNA and birth outcomes were not statistically significant (PFDR≥0.05). However, sex-specific associations were observed for early pregnancy measures of 37 miRNA and GA at birth (PFDR for interactions<0.050). None of the miRNA were associated with fetal growth measures (PFDR≥0.050). Our findings suggest that EV miRNA in both early and late pregnancy may influence gestational duration.http://dx.doi.org/10.1080/15592294.2021.1899887mirnaextracellular vesiclespregnancybirth outcomes |
| spellingShingle | Caitlin G Howe Helen B Foley Elizabeth M Kennedy Sandrah P Eckel Thomas a Chavez Dema Faham Brendan H Grubbs Laila Al-Marayati Deborah Lerner Shakira Suglia Theresa M Bastain Carmen J Marsit Carrie V Breton Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES study Epigenetics mirna extracellular vesicles pregnancy birth outcomes |
| title | Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES study |
| title_full | Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES study |
| title_fullStr | Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES study |
| title_full_unstemmed | Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES study |
| title_short | Extracellular vesicle microRNA in early versus late pregnancy with birth outcomes in the MADRES study |
| title_sort | extracellular vesicle microrna in early versus late pregnancy with birth outcomes in the madres study |
| topic | mirna extracellular vesicles pregnancy birth outcomes |
| url | http://dx.doi.org/10.1080/15592294.2021.1899887 |
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