Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications
Polymethyl methacrylate (PMMA) bone cement (PBC) is commonly used in orthopaedic surgery. However, polymerization volumetric shrinkage, exothermic injury, and low bioactivity prevent PBC from being an ideal material. The developed expandable P(MMA-AA-St) well overcomes the volumetric shrinkage of PB...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422002988 |
| _version_ | 1797938033971953664 |
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| author | Quan-Chang Tan Xia-Shu Jiang Lei Chen Jin-Feng Huang Qiu-Xia Zhou Jing Wang Yan Zhao Bo Zhang Ya-Ni Sun Min Wei Xiong Zhao Zhao Yang Wei Lei Yu-Fei Tang Zi-Xiang Wu |
| author_facet | Quan-Chang Tan Xia-Shu Jiang Lei Chen Jin-Feng Huang Qiu-Xia Zhou Jing Wang Yan Zhao Bo Zhang Ya-Ni Sun Min Wei Xiong Zhao Zhao Yang Wei Lei Yu-Fei Tang Zi-Xiang Wu |
| author_sort | Quan-Chang Tan |
| collection | DOAJ |
| description | Polymethyl methacrylate (PMMA) bone cement (PBC) is commonly used in orthopaedic surgery. However, polymerization volumetric shrinkage, exothermic injury, and low bioactivity prevent PBC from being an ideal material. The developed expandable P(MMA-AA-St) well overcomes the volumetric shrinkage of PBC. However, its biomechanical properties are unsatisfactory. Herein, graphene oxide (GO), a hydrophilic material with favourable biomechanics and osteogenic capability, was added to P(MMA-AA-St) to optimize its biomechanics and bioactivity. The GO-modified self-expandable P(MMA-AA-St)-GO nanocomposite (PGBCs) exhibited outstanding compressive strength (>70 MPa), water absorption, and volume expansion, as well as a longer handling time and a reduced setting temperature. The cytocompatibility of PGBCs was superior to that of PBC, as demonstrated by CCK-8 assay, live-dead cell staining, and flow cytometry. In addition, better osteoblast attachment was observed, which could be attributed to the effects of GO. The improved level of osteogenic gene and protein expression further illustrated the improved cell-material interactions between osteoblasts and PGBCs. The results of an in vivo study performed by filling bone defects in the femoral condyles of rabbits with PGBCs demonstrated promising intraoperative handling properties and convenient implantation. Blood testing and histological staining demonstrated satisfactory in vivo biosafety. Furthermore, bone morphological and microarchitecture analyses using bone tissue staining and micro-CT scanning revealed better bone-PGBCs contact and osteogenic capability. The results of this study indicate that GO modification improved the physiochemical properties, cytocompatibility, and osteogenic capability of P(MMA-AA-St) and overcame the drawbacks of PBC, allowing its material derivatives to serve as effective implantable biomaterials. |
| first_indexed | 2024-04-10T18:54:28Z |
| format | Article |
| id | doaj.art-3fd893ec82c14b749ec162d84ddb625d |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-10T18:54:28Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-3fd893ec82c14b749ec162d84ddb625d2023-02-01T04:27:16ZengElsevierMaterials Today Bio2590-00642023-02-0118100500Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applicationsQuan-Chang Tan0Xia-Shu Jiang1Lei Chen2Jin-Feng Huang3Qiu-Xia Zhou4Jing Wang5Yan Zhao6Bo Zhang7Ya-Ni Sun8Min Wei9Xiong Zhao10Zhao Yang11Wei Lei12Yu-Fei Tang13Zi-Xiang Wu14Department of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR China; Department of Orthopaedics, Air Force Hospital of Eastern Theater Command, Malujie Road No. 1, Nanjing, Jiangsu Province, PR ChinaSchool of Materials Science and Engineering, The Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, PR ChinaSchool of Materials Science and Engineering, The Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, PR ChinaDepartment of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR ChinaSchool of Materials Science and Engineering, The Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, PR ChinaDepartment of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR ChinaDepartment of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR ChinaSchool of Materials Science and Engineering, The Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, PR ChinaSchool of Materials Science and Engineering, The Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, PR ChinaSchool of Materials Science and Engineering, The Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, PR ChinaDepartment of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR ChinaDepartment of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR ChinaDepartment of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR China; Corresponding author. Department of Orthopaedics, Xijing Hospital, No. 127 Changlexi Road, Xi'an Shaanxi Province, 710032, PR China.School of Materials Science and Engineering, The Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, PR China; Corresponding author. School of Materials Science and Engineering, Xi'an University of Technology, No. 5 Jinhua South Road, Xi'an, Shaanxi Province, 710048, PR China.Department of Orthopaedics, Xijing Hospital, The Air Force Medical University, Changlexi Road No. 127, Xi'an, Shaanxi Province, PR China; Corresponding author. Department of Orthopaedics, Xijing Hospital, No. 127 Changlexi Road, Xi'an, Shaanxi Province, 710032, PR China.Polymethyl methacrylate (PMMA) bone cement (PBC) is commonly used in orthopaedic surgery. However, polymerization volumetric shrinkage, exothermic injury, and low bioactivity prevent PBC from being an ideal material. The developed expandable P(MMA-AA-St) well overcomes the volumetric shrinkage of PBC. However, its biomechanical properties are unsatisfactory. Herein, graphene oxide (GO), a hydrophilic material with favourable biomechanics and osteogenic capability, was added to P(MMA-AA-St) to optimize its biomechanics and bioactivity. The GO-modified self-expandable P(MMA-AA-St)-GO nanocomposite (PGBCs) exhibited outstanding compressive strength (>70 MPa), water absorption, and volume expansion, as well as a longer handling time and a reduced setting temperature. The cytocompatibility of PGBCs was superior to that of PBC, as demonstrated by CCK-8 assay, live-dead cell staining, and flow cytometry. In addition, better osteoblast attachment was observed, which could be attributed to the effects of GO. The improved level of osteogenic gene and protein expression further illustrated the improved cell-material interactions between osteoblasts and PGBCs. The results of an in vivo study performed by filling bone defects in the femoral condyles of rabbits with PGBCs demonstrated promising intraoperative handling properties and convenient implantation. Blood testing and histological staining demonstrated satisfactory in vivo biosafety. Furthermore, bone morphological and microarchitecture analyses using bone tissue staining and micro-CT scanning revealed better bone-PGBCs contact and osteogenic capability. The results of this study indicate that GO modification improved the physiochemical properties, cytocompatibility, and osteogenic capability of P(MMA-AA-St) and overcame the drawbacks of PBC, allowing its material derivatives to serve as effective implantable biomaterials.http://www.sciencedirect.com/science/article/pii/S2590006422002988Expandable materialGraphene oxideWater absorptionBiomechanicsOsteointegration |
| spellingShingle | Quan-Chang Tan Xia-Shu Jiang Lei Chen Jin-Feng Huang Qiu-Xia Zhou Jing Wang Yan Zhao Bo Zhang Ya-Ni Sun Min Wei Xiong Zhao Zhao Yang Wei Lei Yu-Fei Tang Zi-Xiang Wu Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications Materials Today Bio Expandable material Graphene oxide Water absorption Biomechanics Osteointegration |
| title | Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications |
| title_full | Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications |
| title_fullStr | Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications |
| title_full_unstemmed | Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications |
| title_short | Bioactive graphene oxide-functionalized self-expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications |
| title_sort | bioactive graphene oxide functionalized self expandable hydrophilic and osteogenic nanocomposite for orthopaedic applications |
| topic | Expandable material Graphene oxide Water absorption Biomechanics Osteointegration |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422002988 |
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