Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell Stress
<i>Leishmania</i> are an understudied genus of parasitic protozoans causing significant health problems for people, particularly in tropical climates. To better understand the growth of <i>Leishmania</i> and potential drug sensitivity implications, the effects of motion on ce...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Stresses |
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| Online Access: | https://www.mdpi.com/2673-7140/3/3/42 |
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| author | Linda M. Ferrence Akshata Gajula Marjorie A. Jones |
| author_facet | Linda M. Ferrence Akshata Gajula Marjorie A. Jones |
| author_sort | Linda M. Ferrence |
| collection | DOAJ |
| description | <i>Leishmania</i> are an understudied genus of parasitic protozoans causing significant health problems for people, particularly in tropical climates. To better understand the growth of <i>Leishmania</i> and potential drug sensitivity implications, the effects of motion on cells grown in vitro were probed. Using a stock <i>Leishmania tarentolae</i> cell culture, cells were placed in 10 mL of a Brain–Heart Infusion medium in either a non-moving (static) environment or on a flat platform of one of two lab rockers (set at a minimal speed) in a dark environment for 13 days. Also, the addition of 0.5 M of L-Proline was evaluated. Microscopy, cell clumping, cell viability, and secreted acid phosphatase (SAP) activity data were collected. Results show that a constant slow rocking motion changed cell growth, clumping behavior, and detectable SAP activity relative to the no-motion cultures, but this change was dependent on which rocker was used, indicating a complexity in the growth of these cells in culture. Thus, continuous motion affects the stresses placed on the cells during a growth curve under some conditions. The implications of this study lead to questions about the effects of motion on the efficacy of pharmaceutical testing in vitro. Further study of the effects of motion on <i>Leishmania</i> is important. |
| first_indexed | 2024-03-10T21:59:13Z |
| format | Article |
| id | doaj.art-3fd93d6e64a04a05bb494f92dbbe7cd4 |
| institution | Directory Open Access Journal |
| issn | 2673-7140 |
| language | English |
| last_indexed | 2024-03-10T21:59:13Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-3fd93d6e64a04a05bb494f92dbbe7cd42023-11-19T13:00:56ZengMDPI AGStresses2673-71402023-08-013360561410.3390/stresses3030042Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell StressLinda M. Ferrence0Akshata Gajula1Marjorie A. Jones2Department of Chemistry, Illinois State University, Normal, IL 61790-4160, USADepartment of Chemistry, Illinois State University, Normal, IL 61790-4160, USADepartment of Chemistry, Illinois State University, Normal, IL 61790-4160, USA<i>Leishmania</i> are an understudied genus of parasitic protozoans causing significant health problems for people, particularly in tropical climates. To better understand the growth of <i>Leishmania</i> and potential drug sensitivity implications, the effects of motion on cells grown in vitro were probed. Using a stock <i>Leishmania tarentolae</i> cell culture, cells were placed in 10 mL of a Brain–Heart Infusion medium in either a non-moving (static) environment or on a flat platform of one of two lab rockers (set at a minimal speed) in a dark environment for 13 days. Also, the addition of 0.5 M of L-Proline was evaluated. Microscopy, cell clumping, cell viability, and secreted acid phosphatase (SAP) activity data were collected. Results show that a constant slow rocking motion changed cell growth, clumping behavior, and detectable SAP activity relative to the no-motion cultures, but this change was dependent on which rocker was used, indicating a complexity in the growth of these cells in culture. Thus, continuous motion affects the stresses placed on the cells during a growth curve under some conditions. The implications of this study lead to questions about the effects of motion on the efficacy of pharmaceutical testing in vitro. Further study of the effects of motion on <i>Leishmania</i> is important.https://www.mdpi.com/2673-7140/3/3/42<i>Leishmania tarentolae</i>rocking motioncell viabilityprolinesecreted acid phosphatase |
| spellingShingle | Linda M. Ferrence Akshata Gajula Marjorie A. Jones Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell Stress Stresses <i>Leishmania tarentolae</i> rocking motion cell viability proline secreted acid phosphatase |
| title | Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell Stress |
| title_full | Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell Stress |
| title_fullStr | Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell Stress |
| title_full_unstemmed | Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell Stress |
| title_short | Surprising Effects of Rocking Motion on <i>Leishmania tarentolae</i> Behavior in Culture and Implications for Cell Stress |
| title_sort | surprising effects of rocking motion on i leishmania tarentolae i behavior in culture and implications for cell stress |
| topic | <i>Leishmania tarentolae</i> rocking motion cell viability proline secreted acid phosphatase |
| url | https://www.mdpi.com/2673-7140/3/3/42 |
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