A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro
The American cranberry, <i>Vaccinium macrocarpon</i>, contains fibers and (poly)phenols that could exert health-promoting effects through modulation of gut microbiota. This study aimed to investigate how a freeze-dried whole cranberry powder (FCP) modulated metabolite production and micr...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | BioTech |
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| Online Access: | https://www.mdpi.com/2673-6284/11/2/14 |
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| author | Christina Khoo Cindy Duysburgh Massimo Marzorati Pieter Van den Abbeele Derek Zhang |
| author_facet | Christina Khoo Cindy Duysburgh Massimo Marzorati Pieter Van den Abbeele Derek Zhang |
| author_sort | Christina Khoo |
| collection | DOAJ |
| description | The American cranberry, <i>Vaccinium macrocarpon</i>, contains fibers and (poly)phenols that could exert health-promoting effects through modulation of gut microbiota. This study aimed to investigate how a freeze-dried whole cranberry powder (FCP) modulated metabolite production and microbial composition using both a 48-h incubation strategy and a long-term human gut simulator study with the M-SHIME (Mucosal Simulator of the Human Intestinal Microbial Ecosystem). FCP was repeatedly administered over three weeks. The studies included five and three study subjects, respectively. In both models, FCP significantly increased levels of health-related short-chain fatty acids (SCFA: acetate, propionate and butyrate), while decreased levels of branched-chain fatty acids (markers of proteolytic fermentation). Interestingly, FCP consistently increased luminal <i>Bacteroidetes</i> abundances in the proximal colon of the M-SHIME (+17.5 ± 9.3%) at the expense of <i>Proteobacteria</i> (−10.2 ± 1.5%). At family level, this was due to the stimulation of <i>Bacteroidaceae</i> and <i>Prevotellaceae</i> and a decrease of <i>Pseudomonodaceae</i> and <i>Enterobacteriaceae</i>. Despite of interpersonal differences, FCP also increased the abundance of families of known butyrate producers. Overall, FCP displayed an interesting prebiotic potential in vitro given its selective utilization by host microorganisms and potential health-related effects on inhibition of pathogens and selective stimulation of beneficial metabolites. |
| first_indexed | 2024-03-10T00:16:48Z |
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| id | doaj.art-3fdce182974e42569305b58bd046da0b |
| institution | Directory Open Access Journal |
| issn | 2673-6284 |
| language | English |
| last_indexed | 2024-03-10T00:16:48Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | BioTech |
| spelling | doaj.art-3fdce182974e42569305b58bd046da0b2023-11-23T15:49:36ZengMDPI AGBioTech2673-62842022-05-011121410.3390/biotech11020014A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In VitroChristina Khoo0Cindy Duysburgh1Massimo Marzorati2Pieter Van den Abbeele3Derek Zhang4Ocean Spray Cranberries, Inc., Bridge Street 152, Middleborough, MA 02349, USAProDigest BV, Technologiepark-Zwijnaarde 73, 9052 Ghent, BelgiumProDigest BV, Technologiepark-Zwijnaarde 73, 9052 Ghent, BelgiumProDigest BV, Technologiepark-Zwijnaarde 73, 9052 Ghent, BelgiumOcean Spray Cranberries, Inc., Bridge Street 152, Middleborough, MA 02349, USAThe American cranberry, <i>Vaccinium macrocarpon</i>, contains fibers and (poly)phenols that could exert health-promoting effects through modulation of gut microbiota. This study aimed to investigate how a freeze-dried whole cranberry powder (FCP) modulated metabolite production and microbial composition using both a 48-h incubation strategy and a long-term human gut simulator study with the M-SHIME (Mucosal Simulator of the Human Intestinal Microbial Ecosystem). FCP was repeatedly administered over three weeks. The studies included five and three study subjects, respectively. In both models, FCP significantly increased levels of health-related short-chain fatty acids (SCFA: acetate, propionate and butyrate), while decreased levels of branched-chain fatty acids (markers of proteolytic fermentation). Interestingly, FCP consistently increased luminal <i>Bacteroidetes</i> abundances in the proximal colon of the M-SHIME (+17.5 ± 9.3%) at the expense of <i>Proteobacteria</i> (−10.2 ± 1.5%). At family level, this was due to the stimulation of <i>Bacteroidaceae</i> and <i>Prevotellaceae</i> and a decrease of <i>Pseudomonodaceae</i> and <i>Enterobacteriaceae</i>. Despite of interpersonal differences, FCP also increased the abundance of families of known butyrate producers. Overall, FCP displayed an interesting prebiotic potential in vitro given its selective utilization by host microorganisms and potential health-related effects on inhibition of pathogens and selective stimulation of beneficial metabolites.https://www.mdpi.com/2673-6284/11/2/14SCFAcranberryprebioticin vitropathogen<i>Akkermansia muciniphila</i> |
| spellingShingle | Christina Khoo Cindy Duysburgh Massimo Marzorati Pieter Van den Abbeele Derek Zhang A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro BioTech SCFA cranberry prebiotic in vitro pathogen <i>Akkermansia muciniphila</i> |
| title | A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro |
| title_full | A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro |
| title_fullStr | A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro |
| title_full_unstemmed | A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro |
| title_short | A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro |
| title_sort | freeze dried cranberry powder consistently enhances scfa production and lowers abundance of opportunistic pathogens in vitro |
| topic | SCFA cranberry prebiotic in vitro pathogen <i>Akkermansia muciniphila</i> |
| url | https://www.mdpi.com/2673-6284/11/2/14 |
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