Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties
Reactive oxygen species and other radicals potentially cause oxidative damage to proteins, lipids, and DNA which may ultimately lead to various complications including mutations, carcinogenesis, neurodegeneration, cardiovascular disease, aging, and inflammatory disease. Recent reports demonstrate th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2017.00276/full |
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| author | Loh Teng-Hern Tan Kok-Gan Chan Tahir Mehmood Khan Tahir Mehmood Khan Sarah Ibrahim Bukhari Surasak Saokaew Surasak Saokaew Surasak Saokaew Surasak Saokaew Acharaporn Duangjai Acharaporn Duangjai Priyia Pusparajah Learn-Han Lee Learn-Han Lee Bey-Hing Goh Bey-Hing Goh |
| author_facet | Loh Teng-Hern Tan Kok-Gan Chan Tahir Mehmood Khan Tahir Mehmood Khan Sarah Ibrahim Bukhari Surasak Saokaew Surasak Saokaew Surasak Saokaew Surasak Saokaew Acharaporn Duangjai Acharaporn Duangjai Priyia Pusparajah Learn-Han Lee Learn-Han Lee Bey-Hing Goh Bey-Hing Goh |
| author_sort | Loh Teng-Hern Tan |
| collection | DOAJ |
| description | Reactive oxygen species and other radicals potentially cause oxidative damage to proteins, lipids, and DNA which may ultimately lead to various complications including mutations, carcinogenesis, neurodegeneration, cardiovascular disease, aging, and inflammatory disease. Recent reports demonstrate that Streptomyces bacteria produce metabolites with potent antioxidant activity that may be developed into therapeutic drugs to combat oxidative stress. This study shows that Streptomyces sp. MUM212 which was isolated from mangrove soil in Kuala Selangor, Malaysia, could be a potential source of antioxidants. Strain MUM212 was characterized and determined as belonging to the genus Streptomyces using 16S rRNA gene phylogenetic analysis. The MUM212 extract demonstrated significant antioxidant activity through DPPH, ABTS and superoxide radical scavenging assays and also metal-chelating activity of 22.03 ± 3.01%, 61.52 ± 3.13%, 37.47 ± 1.79%, and 41.98 ± 0.73% at 4 mg/mL, respectively. Moreover, MUM212 extract was demonstrated to inhibit lipid peroxidation up to 16.72 ± 2.64% at 4 mg/mL and restore survival of Vero cells from H2O2-induced oxidative damages. The antioxidant activities from the MUM212 extract correlated well with its total phenolic contents; and this in turn was in keeping with the gas chromatography–mass spectrometry analysis which revealed the presence of phenolic compounds that could be responsible for the antioxidant properties of the extract. Other chemical constituents detected included hydrocarbons, alcohols and cyclic dipeptides which may have contributed to the overall antioxidant capacity of MUM212 extract. As a whole, strain MUM212 seems to have potential as a promising source of novel molecules for future development of antioxidative therapeutic agents against oxidative stress-related diseases. |
| first_indexed | 2024-12-20T12:21:43Z |
| format | Article |
| id | doaj.art-3fde621c9fab4b3085a54c053f98933a |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-20T12:21:43Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-3fde621c9fab4b3085a54c053f98933a2022-12-21T19:40:58ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122017-05-01810.3389/fphar.2017.00276246601Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating PropertiesLoh Teng-Hern Tan0Kok-Gan Chan1Tahir Mehmood Khan2Tahir Mehmood Khan3Sarah Ibrahim Bukhari4Surasak Saokaew5Surasak Saokaew6Surasak Saokaew7Surasak Saokaew8Acharaporn Duangjai9Acharaporn Duangjai10Priyia Pusparajah11Learn-Han Lee12Learn-Han Lee13Bey-Hing Goh14Bey-Hing Goh15Novel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of MalayaKuala Lumpur, MalaysiaNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaDepartment of Pharmacy, Abasyn UniversityPeshawar, PakistanDepartment of Pharmaceutics, College of Pharmacy, King Saud UniversityRiyadh, Saudi ArabiaNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandPharmaceutical Outcomes Research Center, Faculty of Pharmaceutical Sciences, Naresuan UniversityPhitsanulok, ThailandUnit of Excellence on Herbal Medicine, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandDivision of Physiology, School of Medical Sciences, University of PhayaoPhayao, ThailandJeffrey Cheah School of Medicine and Health Sciences, Monash University MalaysiaBandar Sunway, MalaysiaNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandNovel Bacteria and Drug Discovery Research Group, School of Pharmacy, Monash University MalaysiaBandar Sunway, MalaysiaCenter of Health Outcomes Research and Therapeutic Safety, School of Pharmaceutical Sciences, University of PhayaoPhayao, ThailandReactive oxygen species and other radicals potentially cause oxidative damage to proteins, lipids, and DNA which may ultimately lead to various complications including mutations, carcinogenesis, neurodegeneration, cardiovascular disease, aging, and inflammatory disease. Recent reports demonstrate that Streptomyces bacteria produce metabolites with potent antioxidant activity that may be developed into therapeutic drugs to combat oxidative stress. This study shows that Streptomyces sp. MUM212 which was isolated from mangrove soil in Kuala Selangor, Malaysia, could be a potential source of antioxidants. Strain MUM212 was characterized and determined as belonging to the genus Streptomyces using 16S rRNA gene phylogenetic analysis. The MUM212 extract demonstrated significant antioxidant activity through DPPH, ABTS and superoxide radical scavenging assays and also metal-chelating activity of 22.03 ± 3.01%, 61.52 ± 3.13%, 37.47 ± 1.79%, and 41.98 ± 0.73% at 4 mg/mL, respectively. Moreover, MUM212 extract was demonstrated to inhibit lipid peroxidation up to 16.72 ± 2.64% at 4 mg/mL and restore survival of Vero cells from H2O2-induced oxidative damages. The antioxidant activities from the MUM212 extract correlated well with its total phenolic contents; and this in turn was in keeping with the gas chromatography–mass spectrometry analysis which revealed the presence of phenolic compounds that could be responsible for the antioxidant properties of the extract. Other chemical constituents detected included hydrocarbons, alcohols and cyclic dipeptides which may have contributed to the overall antioxidant capacity of MUM212 extract. As a whole, strain MUM212 seems to have potential as a promising source of novel molecules for future development of antioxidative therapeutic agents against oxidative stress-related diseases.http://journal.frontiersin.org/article/10.3389/fphar.2017.00276/fullStreptomyces sp.Malaysiamangroveantioxidantradical-scavenging |
| spellingShingle | Loh Teng-Hern Tan Kok-Gan Chan Tahir Mehmood Khan Tahir Mehmood Khan Sarah Ibrahim Bukhari Surasak Saokaew Surasak Saokaew Surasak Saokaew Surasak Saokaew Acharaporn Duangjai Acharaporn Duangjai Priyia Pusparajah Learn-Han Lee Learn-Han Lee Bey-Hing Goh Bey-Hing Goh Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties Frontiers in Pharmacology Streptomyces sp. Malaysia mangrove antioxidant radical-scavenging |
| title | Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties |
| title_full | Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties |
| title_fullStr | Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties |
| title_full_unstemmed | Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties |
| title_short | Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties |
| title_sort | streptomyces sp mum212 as a source of antioxidants with radical scavenging and metal chelating properties |
| topic | Streptomyces sp. Malaysia mangrove antioxidant radical-scavenging |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2017.00276/full |
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