Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum
The aim of the present study was to isolate endophytic fungi and examine their anti-diabetic as well as antioxidant potential. Twenty-nine endophytic fungi were isolated from the stem and root parts of Bryophyllum pinnatum. Based on the presence of phytoconstituents, 16 endophytic isolates were tes...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2023-06-01
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| Series: | Bangladesh Journal of Pharmacology |
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| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/65836 |
| _version_ | 1797799482409091072 |
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| author | Sugashini Settu Sathiavelu Arunachalam |
| author_facet | Sugashini Settu Sathiavelu Arunachalam |
| author_sort | Sugashini Settu |
| collection | DOAJ |
| description |
The aim of the present study was to isolate endophytic fungi and examine their anti-diabetic as well as antioxidant potential. Twenty-nine endophytic fungi were isolated from the stem and root parts of Bryophyllum pinnatum. Based on the presence of phytoconstituents, 16 endophytic isolates were tested for the inhibition of α-amylase enzyme and free radicals. Thirteen isolates showed significant inhibition of α-amylase enzyme (18.8 ± 0.8 to 62.5 ± 0.4%) and free radicals (15.3 ± 0.2 to 87.4 ± 0.8%). Good inhibitions were found in six isolates, which was further tested to validates its anti-diabetic activity by glucose uptake in yeast cell and glucose diffusion inhibition studies. The most active isolates were identified as Daldinia eschscholtzii (BPS17) and Phomopsis mangiferae (BPK9) by microscopic and molecular identification method. Identified fungal isolates were tested for glucose uptake using 3T3 cell lines (100 µg/mL) and the result showed effective glucose utilization. These finding provides evidence that D. eschscholtzii and P. mangiferae are potent endophytic fungi having anti-diabetic activity.
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| first_indexed | 2024-03-13T04:20:34Z |
| format | Article |
| id | doaj.art-3fe0fa8453ae46f2a0f9c30bb642eaf8 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-03-13T04:20:34Z |
| publishDate | 2023-06-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-3fe0fa8453ae46f2a0f9c30bb642eaf82023-06-20T10:13:14ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882023-06-0118210.3329/bjp.v18i2.65836Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatumSugashini Settu0Sathiavelu Arunachalam1Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore 14, India.Department of Agriculture Microbiology, Vellore Institute of Technology School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 14, India. The aim of the present study was to isolate endophytic fungi and examine their anti-diabetic as well as antioxidant potential. Twenty-nine endophytic fungi were isolated from the stem and root parts of Bryophyllum pinnatum. Based on the presence of phytoconstituents, 16 endophytic isolates were tested for the inhibition of α-amylase enzyme and free radicals. Thirteen isolates showed significant inhibition of α-amylase enzyme (18.8 ± 0.8 to 62.5 ± 0.4%) and free radicals (15.3 ± 0.2 to 87.4 ± 0.8%). Good inhibitions were found in six isolates, which was further tested to validates its anti-diabetic activity by glucose uptake in yeast cell and glucose diffusion inhibition studies. The most active isolates were identified as Daldinia eschscholtzii (BPS17) and Phomopsis mangiferae (BPK9) by microscopic and molecular identification method. Identified fungal isolates were tested for glucose uptake using 3T3 cell lines (100 µg/mL) and the result showed effective glucose utilization. These finding provides evidence that D. eschscholtzii and P. mangiferae are potent endophytic fungi having anti-diabetic activity. https://www.banglajol.info/index.php/BJP/article/view/65836Alpha-amylaseAnti-diabeticBryophyllum pinnatum3T3 cell lineDaldinia eschscholtziiDPPH |
| spellingShingle | Sugashini Settu Sathiavelu Arunachalam Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum Bangladesh Journal of Pharmacology Alpha-amylase Anti-diabetic Bryophyllum pinnatum 3T3 cell line Daldinia eschscholtzii DPPH |
| title | Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum |
| title_full | Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum |
| title_fullStr | Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum |
| title_full_unstemmed | Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum |
| title_short | Anti-diabetic effect of Daldinia eschscholtzii and Phomopsis mangiferae isolated from Bryophyllum pinnatum |
| title_sort | anti diabetic effect of daldinia eschscholtzii and phomopsis mangiferae isolated from bryophyllum pinnatum |
| topic | Alpha-amylase Anti-diabetic Bryophyllum pinnatum 3T3 cell line Daldinia eschscholtzii DPPH |
| url | https://www.banglajol.info/index.php/BJP/article/view/65836 |
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