Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe
In this paper, a novel, accurate, sensitive and rapid ratiometric fluorescent sensor was fabricated using a copper nanoclusters@infinite coordination polymer (ICP), specifically, terbium ion-guanosine 5’-disodium (Cu NCs@Tb-GMP) nanocomposites as the ratiometric fluorescent probe, to detect alkaline...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2079-6374/12/6/372 |
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| author | Xin Li Xiaoling Wang Wei Guo Yunfei Wang Qing Hua Feiyan Tang Feng Luan Chunyuan Tian Xuming Zhuang Lijun Zhao |
| author_facet | Xin Li Xiaoling Wang Wei Guo Yunfei Wang Qing Hua Feiyan Tang Feng Luan Chunyuan Tian Xuming Zhuang Lijun Zhao |
| author_sort | Xin Li |
| collection | DOAJ |
| description | In this paper, a novel, accurate, sensitive and rapid ratiometric fluorescent sensor was fabricated using a copper nanoclusters@infinite coordination polymer (ICP), specifically, terbium ion-guanosine 5’-disodium (Cu NCs@Tb-GMP) nanocomposites as the ratiometric fluorescent probe, to detect alkaline phosphatase (ALP) in water. The fluorescence probe was characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The experimental results showed that, compared with Tb-GMP fluorescent sensors, Cu ratiometric fluorescent sensors based on NCs encapsulated in Tb-GMP had fewer experimental errors and fewer false-positive signals and were more conducive to the sensitive and accurate detection of ALP. In addition, the developed fluorescent probe had good fluorescence intensity, selectivity, repeatability and stability. Under optimized conditions, the ratiometric fluorescent sensor detected ALP in the range of 0.002–2 U mL<sup>−1</sup> (R<sup>2</sup> = 0.9950) with a limit of detection of 0.002 U mL<sup>−1</sup>, and the recovery of ALP from water samples was less than 108.2%. These satisfying results proved that the ratiometric fluorescent probe has good application prospects and provides a new method for the detection of ALP in real water samples. |
| first_indexed | 2024-03-10T00:17:46Z |
| format | Article |
| id | doaj.art-3fe117cd04cc42c4937d84f7295c1212 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:17:46Z |
| publishDate | 2022-05-01 |
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| series | Biosensors |
| spelling | doaj.art-3fe117cd04cc42c4937d84f7295c12122023-11-23T15:48:32ZengMDPI AGBiosensors2079-63742022-05-0112637210.3390/bios12060372Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent ProbeXin Li0Xiaoling Wang1Wei Guo2Yunfei Wang3Qing Hua4Feiyan Tang5Feng Luan6Chunyuan Tian7Xuming Zhuang8Lijun Zhao9College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaShandong Dyne Marine Biopharmaceutical Co., Ltd., Weihai 264300, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaCollege of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, ChinaIn this paper, a novel, accurate, sensitive and rapid ratiometric fluorescent sensor was fabricated using a copper nanoclusters@infinite coordination polymer (ICP), specifically, terbium ion-guanosine 5’-disodium (Cu NCs@Tb-GMP) nanocomposites as the ratiometric fluorescent probe, to detect alkaline phosphatase (ALP) in water. The fluorescence probe was characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The experimental results showed that, compared with Tb-GMP fluorescent sensors, Cu ratiometric fluorescent sensors based on NCs encapsulated in Tb-GMP had fewer experimental errors and fewer false-positive signals and were more conducive to the sensitive and accurate detection of ALP. In addition, the developed fluorescent probe had good fluorescence intensity, selectivity, repeatability and stability. Under optimized conditions, the ratiometric fluorescent sensor detected ALP in the range of 0.002–2 U mL<sup>−1</sup> (R<sup>2</sup> = 0.9950) with a limit of detection of 0.002 U mL<sup>−1</sup>, and the recovery of ALP from water samples was less than 108.2%. These satisfying results proved that the ratiometric fluorescent probe has good application prospects and provides a new method for the detection of ALP in real water samples.https://www.mdpi.com/2079-6374/12/6/372alkaline phosphatasecopper nanoclustersinfinite coordination polymerratiometricfluorescent probe |
| spellingShingle | Xin Li Xiaoling Wang Wei Guo Yunfei Wang Qing Hua Feiyan Tang Feng Luan Chunyuan Tian Xuming Zhuang Lijun Zhao Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe Biosensors alkaline phosphatase copper nanoclusters infinite coordination polymer ratiometric fluorescent probe |
| title | Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe |
| title_full | Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe |
| title_fullStr | Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe |
| title_full_unstemmed | Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe |
| title_short | Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe |
| title_sort | selective detection of alkaline phosphatase activity in environmental water samples by copper nanoclusters doped lanthanide coordination polymer nanocomposites as the ratiometric fluorescent probe |
| topic | alkaline phosphatase copper nanoclusters infinite coordination polymer ratiometric fluorescent probe |
| url | https://www.mdpi.com/2079-6374/12/6/372 |
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