Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i>
Reducing the oxidative stress in neurons extends lifespan in <i>Drosophila melanogaster</i>, highlighting the crucial role of neuronal oxidative damage in lifespan determination. However, the source of the reactive oxygen species (ROS) that provoke oxidative stress in neurons is not clea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/13/2059 |
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| author | Minwoo Baek Wijeong Jang Changsoo Kim |
| author_facet | Minwoo Baek Wijeong Jang Changsoo Kim |
| author_sort | Minwoo Baek |
| collection | DOAJ |
| description | Reducing the oxidative stress in neurons extends lifespan in <i>Drosophila melanogaster</i>, highlighting the crucial role of neuronal oxidative damage in lifespan determination. However, the source of the reactive oxygen species (ROS) that provoke oxidative stress in neurons is not clearly defined. Here, we identify <i>dual oxidase</i> (<i>duox</i>), a calcium-activated ROS-producing enzyme, as a lifespan determinant. Due to the lethality of <i>duox</i> homozygous mutants, we employed a <i>duox</i> heterozygote that exhibited normal appearance and movement. We found that <i>duox</i> heterozygous male flies, which were isogenized with control flies, demonstrated extended lifespan. Neuronal knockdown experiments further suggested that <i>duox</i> is crucial to oxidative stress in neurons. Our findings suggest <i>duox</i> to be a source of neuronal oxidative stress associated with animal lifespan. |
| first_indexed | 2024-03-09T22:01:24Z |
| format | Article |
| id | doaj.art-3fe1240db7a846cf8e569918dfb667fe |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T22:01:24Z |
| publishDate | 2022-06-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-3fe1240db7a846cf8e569918dfb667fe2023-11-23T19:48:50ZengMDPI AGCells2073-44092022-06-011113205910.3390/cells11132059Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i>Minwoo Baek0Wijeong Jang1Changsoo Kim2School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, KoreaSchool of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, KoreaSchool of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, KoreaReducing the oxidative stress in neurons extends lifespan in <i>Drosophila melanogaster</i>, highlighting the crucial role of neuronal oxidative damage in lifespan determination. However, the source of the reactive oxygen species (ROS) that provoke oxidative stress in neurons is not clearly defined. Here, we identify <i>dual oxidase</i> (<i>duox</i>), a calcium-activated ROS-producing enzyme, as a lifespan determinant. Due to the lethality of <i>duox</i> homozygous mutants, we employed a <i>duox</i> heterozygote that exhibited normal appearance and movement. We found that <i>duox</i> heterozygous male flies, which were isogenized with control flies, demonstrated extended lifespan. Neuronal knockdown experiments further suggested that <i>duox</i> is crucial to oxidative stress in neurons. Our findings suggest <i>duox</i> to be a source of neuronal oxidative stress associated with animal lifespan.https://www.mdpi.com/2073-4409/11/13/2059lifespanduoxROS<i>Drosophila melanogaster</i> |
| spellingShingle | Minwoo Baek Wijeong Jang Changsoo Kim Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i> Cells lifespan duox ROS <i>Drosophila melanogaster</i> |
| title | Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i> |
| title_full | Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i> |
| title_fullStr | Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i> |
| title_full_unstemmed | Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i> |
| title_short | Dual Oxidase, a Hydrogen-Peroxide-Producing Enzyme, Regulates Neuronal Oxidative Damage and Animal Lifespan in <i>Drosophila melanogaster</i> |
| title_sort | dual oxidase a hydrogen peroxide producing enzyme regulates neuronal oxidative damage and animal lifespan in i drosophila melanogaster i |
| topic | lifespan duox ROS <i>Drosophila melanogaster</i> |
| url | https://www.mdpi.com/2073-4409/11/13/2059 |
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