Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing
As an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the ne...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/1/209 |
| _version_ | 1811187765714354176 |
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| author | Ruixin Jiang Huihuang Wu Jianpeng Yang Haiyan Jiang Min Du Mangi Vai Siohang Pun Yueming Gao |
| author_facet | Ruixin Jiang Huihuang Wu Jianpeng Yang Haiyan Jiang Min Du Mangi Vai Siohang Pun Yueming Gao |
| author_sort | Ruixin Jiang |
| collection | DOAJ |
| description | As an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the needs of point-of-care testing because of its simple operation, portability and fast detection speed. However, the image gray level of common image sensors limits the detection range of the FICA reader, and high-precision image sensors are expensive, which is not conducive to the popularization of the instrument. In this paper, FICA strips’ image was collected using a common complementary metal oxide semiconductor (CMOS) image sensor and a range adjustment mechanism was established to automatically adjust the exposure time of the CMOS image sensor to achieve the effect of range expansion. The detection sensitivity showed a onefold increase, and the upper detection limit showed a twofold increase after the proposed method was implemented. In addition, in the experiments of linearity and accuracy, the fitting degree (<i>R</i><sup>2</sup>) of the fitted curves both reached 0.999. Therefore, the automatic range adjustment method can obviously improve the detection range of the FICA reader based on image processing. |
| first_indexed | 2024-04-11T14:09:06Z |
| format | Article |
| id | doaj.art-3fe5ab020c964c968f098650ef62389a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T14:09:06Z |
| publishDate | 2019-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-3fe5ab020c964c968f098650ef62389a2022-12-22T04:19:48ZengMDPI AGSensors1424-82202019-12-0120120910.3390/s20010209s20010209Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image ProcessingRuixin Jiang0Huihuang Wu1Jianpeng Yang2Haiyan Jiang3Min Du4Mangi Vai5Siohang Pun6Yueming Gao7College of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, ChinaCollege of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, ChinaCollege of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, ChinaKey Lab of Medical Instrumentation & Pharmaceutical Technology of Fujian Province, Fuzhou 350108, ChinaCollege of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, ChinaKey Lab of Medical Instrumentation & Pharmaceutical Technology of Fujian Province, Fuzhou 350108, ChinaState Key Laboratory of Analog and Mixed-Signal VLSI, University of Macau, Macau 999078, Macau SAR, ChinaCollege of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, ChinaAs an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the needs of point-of-care testing because of its simple operation, portability and fast detection speed. However, the image gray level of common image sensors limits the detection range of the FICA reader, and high-precision image sensors are expensive, which is not conducive to the popularization of the instrument. In this paper, FICA strips’ image was collected using a common complementary metal oxide semiconductor (CMOS) image sensor and a range adjustment mechanism was established to automatically adjust the exposure time of the CMOS image sensor to achieve the effect of range expansion. The detection sensitivity showed a onefold increase, and the upper detection limit showed a twofold increase after the proposed method was implemented. In addition, in the experiments of linearity and accuracy, the fitting degree (<i>R</i><sup>2</sup>) of the fitted curves both reached 0.999. Therefore, the automatic range adjustment method can obviously improve the detection range of the FICA reader based on image processing.https://www.mdpi.com/1424-8220/20/1/209fluorescence immunochromatographic assaypoint-of-care testingautomatic range adjustmentimage processingexposure time |
| spellingShingle | Ruixin Jiang Huihuang Wu Jianpeng Yang Haiyan Jiang Min Du Mangi Vai Siohang Pun Yueming Gao Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing Sensors fluorescence immunochromatographic assay point-of-care testing automatic range adjustment image processing exposure time |
| title | Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing |
| title_full | Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing |
| title_fullStr | Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing |
| title_full_unstemmed | Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing |
| title_short | Automatic Range Adjustment of the Fluorescence Immunochromatographic Assay Based on Image Processing |
| title_sort | automatic range adjustment of the fluorescence immunochromatographic assay based on image processing |
| topic | fluorescence immunochromatographic assay point-of-care testing automatic range adjustment image processing exposure time |
| url | https://www.mdpi.com/1424-8220/20/1/209 |
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