Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay
Tuberculosis (TB) remains a main hurdle for national programs due to increase in drug resistance to antitubercular drugs. World Health Organization (WHO)-endorsed Line Probe Assay, Genotype MTBDRsl Ver 2.0, gives opportunity for rapid diagnosis and molecular characterization of different mutations i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2020-01-01
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| Series: | Journal of Epidemiology and Global Health |
| Subjects: | |
| Online Access: | https://www.atlantis-press.com/article/125931650/view |
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| author | Sunil Sethi Priyanka Agarwal Rajiv Khaneja Naresh Kumar Nitin Kumar Jagesh Chandna Ashutosh Nath Aggarwal Rakesh Yadav |
| author_facet | Sunil Sethi Priyanka Agarwal Rajiv Khaneja Naresh Kumar Nitin Kumar Jagesh Chandna Ashutosh Nath Aggarwal Rakesh Yadav |
| author_sort | Sunil Sethi |
| collection | DOAJ |
| description | Tuberculosis (TB) remains a main hurdle for national programs due to increase in drug resistance to antitubercular drugs. World Health Organization (WHO)-endorsed Line Probe Assay, Genotype MTBDRsl Ver 2.0, gives opportunity for rapid diagnosis and molecular characterization of different mutations in drug targets of fluoroquinolone (FQ) and second-line injectable drugs (SLID). We, retrospectively, analyzed the data of Genotype MTBDRsl Ver 2.0 from January 2018 to June 2018. A total of 863 isolates of Mycobacterium tuberculosis, 687 rifampicin resistant and 176 isoniazid resistant only, were screened for drug resistance in FQ and SLID. All the isolates were tested for Genotype MTBDRsl Ver 2.0 according to the manufacturer’s instructions. The FQ and SLID resistance were detected in 295 (34.2%) and 70 (8.1%) isolates, respectively. Among newly diagnosed and follow-up rifampicin-resistant TB (RR TB) patients, the FQ resistance was 25.8% and 44.5%, respectively. The most common mutation (42.7%) in FQ-resistant isolates was MUT3C in gyrA gene. Both SLID and FQ resistance were detected in 59 (6.8%) RR TB isolates. The mono SLID resistance was detected in 12 (1.7%) isolates of RR TB. Genotype MTBDRsl Ver 2.0 assay is a rapid and important tool for the diagnosis and molecular characterization of second-line drug resistance under programmatic conditions. |
| first_indexed | 2024-04-14T06:30:33Z |
| format | Article |
| id | doaj.art-3fe6b4d456954ed798ac665442beddb0 |
| institution | Directory Open Access Journal |
| issn | 2210-6014 |
| language | English |
| last_indexed | 2024-04-14T06:30:33Z |
| publishDate | 2020-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Epidemiology and Global Health |
| spelling | doaj.art-3fe6b4d456954ed798ac665442beddb02022-12-22T02:07:38ZengSpringerJournal of Epidemiology and Global Health2210-60142020-01-0110110.2991/jegh.k.191215.003Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl AssaySunil SethiPriyanka AgarwalRajiv KhanejaNaresh KumarNitin KumarJagesh ChandnaAshutosh Nath AggarwalRakesh YadavTuberculosis (TB) remains a main hurdle for national programs due to increase in drug resistance to antitubercular drugs. World Health Organization (WHO)-endorsed Line Probe Assay, Genotype MTBDRsl Ver 2.0, gives opportunity for rapid diagnosis and molecular characterization of different mutations in drug targets of fluoroquinolone (FQ) and second-line injectable drugs (SLID). We, retrospectively, analyzed the data of Genotype MTBDRsl Ver 2.0 from January 2018 to June 2018. A total of 863 isolates of Mycobacterium tuberculosis, 687 rifampicin resistant and 176 isoniazid resistant only, were screened for drug resistance in FQ and SLID. All the isolates were tested for Genotype MTBDRsl Ver 2.0 according to the manufacturer’s instructions. The FQ and SLID resistance were detected in 295 (34.2%) and 70 (8.1%) isolates, respectively. Among newly diagnosed and follow-up rifampicin-resistant TB (RR TB) patients, the FQ resistance was 25.8% and 44.5%, respectively. The most common mutation (42.7%) in FQ-resistant isolates was MUT3C in gyrA gene. Both SLID and FQ resistance were detected in 59 (6.8%) RR TB isolates. The mono SLID resistance was detected in 12 (1.7%) isolates of RR TB. Genotype MTBDRsl Ver 2.0 assay is a rapid and important tool for the diagnosis and molecular characterization of second-line drug resistance under programmatic conditions.https://www.atlantis-press.com/article/125931650/viewDrug resistancediagnosisMTBDRsl Ver 2.0Mycobacterium tuberculosis |
| spellingShingle | Sunil Sethi Priyanka Agarwal Rajiv Khaneja Naresh Kumar Nitin Kumar Jagesh Chandna Ashutosh Nath Aggarwal Rakesh Yadav Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay Journal of Epidemiology and Global Health Drug resistance diagnosis MTBDRsl Ver 2.0 Mycobacterium tuberculosis |
| title | Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay |
| title_full | Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay |
| title_fullStr | Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay |
| title_full_unstemmed | Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay |
| title_short | Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay |
| title_sort | second line drug resistance characterization in mycobacterium tuberculosis by genotype mtbdrsl assay |
| topic | Drug resistance diagnosis MTBDRsl Ver 2.0 Mycobacterium tuberculosis |
| url | https://www.atlantis-press.com/article/125931650/view |
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