<i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L.
<i>Phaseolus vulgaris</i> L. (Fabaceae) forms symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing rhizobia (NFB). The tripartite relationship uses molecular singals to establish intracellular symbioses in roots. The goal of this study was to determine if <i>Rhizob...
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| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/3/876 |
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| author | Jacob Banuelos Esperanza Martínez-Romero Noé Manuel Montaño Sara Lucía Camargo-Ricalde |
| author_facet | Jacob Banuelos Esperanza Martínez-Romero Noé Manuel Montaño Sara Lucía Camargo-Ricalde |
| author_sort | Jacob Banuelos |
| collection | DOAJ |
| description | <i>Phaseolus vulgaris</i> L. (Fabaceae) forms symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing rhizobia (NFB). The tripartite relationship uses molecular singals to establish intracellular symbioses in roots. The goal of this study was to determine if <i>Rhizobium tropici</i> CIAT 899 and exogenous riboflavin (vitamin B2) have an effect on AMF species selection and root colonization of <i>P. vulgaris</i>. Using SSU rRNA fragment amplification of DNA extracted from <i>P. vulgaris</i> roots, we found that the presence of <i>R. tropici</i> altered the relative distribution of AMF species. <i>Dominikia bernensis</i> (Ohel) was the most abundant AMF species in <i>P. vulgaris</i> roots but when <i>R. tropici</i> was co-inoculated, <i>Glomus</i> species dominated. Rhizobacteria such as <i>R. tropici</i>, secrete riboflavin and could affect AMF symbiosis. Addition of 50 μM riboflavin to <i>P. vulgaris,</i> increased plant growth (28%), dry nodule weight (18%), AMF colonization (248%) and mycorrhizal vesicle frequency (56%) in bean roots. 3.12 and 12.5 µM riboflavin favored the presence of <i>Glomus macrocarpum</i> in <i>P. vulgaris</i> roots. This work provides the basis to further study of rhizobial and mycorrhizal co-inoculation of <i>Phaseolus vulgaris</i> bean. |
| first_indexed | 2024-03-11T07:02:23Z |
| format | Article |
| id | doaj.art-3fe8789e644d4035a5c8aeffd10a9a68 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T07:02:23Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-3fe8789e644d4035a5c8aeffd10a9a682023-11-17T09:07:30ZengMDPI AGAgronomy2073-43952023-03-0113387610.3390/agronomy13030876<i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L.Jacob Banuelos0Esperanza Martínez-Romero1Noé Manuel Montaño2Sara Lucía Camargo-Ricalde3Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Av. Ferrocarill San Rafael Atlixco 186, Mexico City 09310, MexicoCentro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca 62210, MexicoDepartamento de Biología, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Av. Ferrocarill san Rafael Atlixco 186, Mexico City 09310, MexicoDepartamento de Biología, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Av. Ferrocarill san Rafael Atlixco 186, Mexico City 09310, Mexico<i>Phaseolus vulgaris</i> L. (Fabaceae) forms symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing rhizobia (NFB). The tripartite relationship uses molecular singals to establish intracellular symbioses in roots. The goal of this study was to determine if <i>Rhizobium tropici</i> CIAT 899 and exogenous riboflavin (vitamin B2) have an effect on AMF species selection and root colonization of <i>P. vulgaris</i>. Using SSU rRNA fragment amplification of DNA extracted from <i>P. vulgaris</i> roots, we found that the presence of <i>R. tropici</i> altered the relative distribution of AMF species. <i>Dominikia bernensis</i> (Ohel) was the most abundant AMF species in <i>P. vulgaris</i> roots but when <i>R. tropici</i> was co-inoculated, <i>Glomus</i> species dominated. Rhizobacteria such as <i>R. tropici</i>, secrete riboflavin and could affect AMF symbiosis. Addition of 50 μM riboflavin to <i>P. vulgaris,</i> increased plant growth (28%), dry nodule weight (18%), AMF colonization (248%) and mycorrhizal vesicle frequency (56%) in bean roots. 3.12 and 12.5 µM riboflavin favored the presence of <i>Glomus macrocarpum</i> in <i>P. vulgaris</i> roots. This work provides the basis to further study of rhizobial and mycorrhizal co-inoculation of <i>Phaseolus vulgaris</i> bean.https://www.mdpi.com/2073-4395/13/3/876arbuscular mycorrhizaspecies compositionriboflavin (vitamin B2)nitrogen fixingvesicles<i>Dominikia</i> |
| spellingShingle | Jacob Banuelos Esperanza Martínez-Romero Noé Manuel Montaño Sara Lucía Camargo-Ricalde <i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L. Agronomy arbuscular mycorrhiza species composition riboflavin (vitamin B2) nitrogen fixing vesicles <i>Dominikia</i> |
| title | <i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L. |
| title_full | <i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L. |
| title_fullStr | <i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L. |
| title_full_unstemmed | <i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L. |
| title_short | <i>Rhizobium tropici</i> and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in <i>Phaseolus vulgaris</i> L. |
| title_sort | i rhizobium tropici i and riboflavin amendment condition arbuscular mycorrhiza colonization in i phaseolus vulgaris i l |
| topic | arbuscular mycorrhiza species composition riboflavin (vitamin B2) nitrogen fixing vesicles <i>Dominikia</i> |
| url | https://www.mdpi.com/2073-4395/13/3/876 |
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