Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. Isolate
In this study, the effect of media composition, N/P ratio and cultivation strategy on the formation of carotenoids in a <i>Coelastrella</i> sp. isolate was investigated. A two-stage process utilizing different media in the vegetative stage, with subsequent re-suspension in medium without...
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/20/6950 |
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| author | Hamdy Elsayed Ahmed Ali Fritz Vorisek Scot E. Dowd Stephanie Kesner Yang Song Dali Qian Mark Crocker |
| author_facet | Hamdy Elsayed Ahmed Ali Fritz Vorisek Scot E. Dowd Stephanie Kesner Yang Song Dali Qian Mark Crocker |
| author_sort | Hamdy Elsayed Ahmed Ali |
| collection | DOAJ |
| description | In this study, the effect of media composition, N/P ratio and cultivation strategy on the formation of carotenoids in a <i>Coelastrella</i> sp. isolate was investigated. A two-stage process utilizing different media in the vegetative stage, with subsequent re-suspension in medium without nitrate, was employed to enhance the formation of carotenoids. The optimal growth and carotenoid content (β-carotene and lutein) in the vegetative phase were obtained by cultivation in M-8 and BG11 media. Use of a N/P ratio of 37.5 and low light intensity of 40 μmol m<sup>−2</sup> s<sup>−1</sup> (control conditions) led to optimal biomass production of up to 1.31 g L<sup>−1</sup>. Low concentrations of astaxanthin (maximum of 0.31 wt. %) were accumulated under stress conditions (nitrogen-deficient medium containing 1.5 % of NaCl and light intensity of 500 μmol m<sup>−2</sup> s<sup>−1</sup>), while β-carotene and lutein (combined maximum of 2.12 wt. %) were produced under non-stress conditions. Lipid analysis revealed that palmitic (C16:0) and oleic (C18:1) constituted the main algal fatty acid chains (50.2 ± 2.1% of the total fatty acids), while esterifiable lipids constituted 17.2 ± 0.5% of the biomass by weight. These results suggest that <i>Coelastrella</i> sp. could also be a promising feedstock for biodiesel production. |
| first_indexed | 2024-03-09T19:42:13Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:42:13Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3fe91095852b4cbb9d9117997c4b57062023-11-24T01:34:30ZengMDPI AGMolecules1420-30492022-10-012720695010.3390/molecules27206950Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. IsolateHamdy Elsayed Ahmed Ali0Fritz Vorisek1Scot E. Dowd2Stephanie Kesner3Yang Song4Dali Qian5Mark Crocker6Center for Applied Energy Research, University of Kentucky, Lexington, KY 40511, USACenter for Applied Energy Research, University of Kentucky, Lexington, KY 40511, USAMR DNA (Molecular Research LP), 503 Clovis Road, Shallowater, TX 79363, USACenter for Applied Energy Research, University of Kentucky, Lexington, KY 40511, USACenter for Applied Energy Research, University of Kentucky, Lexington, KY 40511, USACenter for Applied Energy Research, University of Kentucky, Lexington, KY 40511, USACenter for Applied Energy Research, University of Kentucky, Lexington, KY 40511, USAIn this study, the effect of media composition, N/P ratio and cultivation strategy on the formation of carotenoids in a <i>Coelastrella</i> sp. isolate was investigated. A two-stage process utilizing different media in the vegetative stage, with subsequent re-suspension in medium without nitrate, was employed to enhance the formation of carotenoids. The optimal growth and carotenoid content (β-carotene and lutein) in the vegetative phase were obtained by cultivation in M-8 and BG11 media. Use of a N/P ratio of 37.5 and low light intensity of 40 μmol m<sup>−2</sup> s<sup>−1</sup> (control conditions) led to optimal biomass production of up to 1.31 g L<sup>−1</sup>. Low concentrations of astaxanthin (maximum of 0.31 wt. %) were accumulated under stress conditions (nitrogen-deficient medium containing 1.5 % of NaCl and light intensity of 500 μmol m<sup>−2</sup> s<sup>−1</sup>), while β-carotene and lutein (combined maximum of 2.12 wt. %) were produced under non-stress conditions. Lipid analysis revealed that palmitic (C16:0) and oleic (C18:1) constituted the main algal fatty acid chains (50.2 ± 2.1% of the total fatty acids), while esterifiable lipids constituted 17.2 ± 0.5% of the biomass by weight. These results suggest that <i>Coelastrella</i> sp. could also be a promising feedstock for biodiesel production.https://www.mdpi.com/1420-3049/27/20/6950algaepigmentsluteinβ-caroteneastaxanthinfatty acids |
| spellingShingle | Hamdy Elsayed Ahmed Ali Fritz Vorisek Scot E. Dowd Stephanie Kesner Yang Song Dali Qian Mark Crocker Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. Isolate Molecules algae pigments lutein β-carotene astaxanthin fatty acids |
| title | Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. Isolate |
| title_full | Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. Isolate |
| title_fullStr | Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. Isolate |
| title_full_unstemmed | Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. Isolate |
| title_short | Formation of Lutein, β-Carotene and Astaxanthin in a <i>Coelastrella</i> sp. Isolate |
| title_sort | formation of lutein β carotene and astaxanthin in a i coelastrella i sp isolate |
| topic | algae pigments lutein β-carotene astaxanthin fatty acids |
| url | https://www.mdpi.com/1420-3049/27/20/6950 |
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