Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells
<i>Artemisia rupestris</i> L. has long been used as a traditional herbal medicine owing to its immunomodulatory activity. Aqueous extracts of <i>Artemisia rupestris</i> L. (AEAR) contain the main functional component and can activate the maturation of dendritic cells (DCs) an...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/3/525 |
| _version_ | 1797553772195479552 |
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| author | Yu Yang DanYang Wang QuanXiao Li Jiang He Bin Wang Jinyao Li Ailian Zhang |
| author_facet | Yu Yang DanYang Wang QuanXiao Li Jiang He Bin Wang Jinyao Li Ailian Zhang |
| author_sort | Yu Yang |
| collection | DOAJ |
| description | <i>Artemisia rupestris</i> L. has long been used as a traditional herbal medicine owing to its immunomodulatory activity. Aqueous extracts of <i>Artemisia rupestris</i> L. (AEAR) contain the main functional component and can activate the maturation of dendritic cells (DCs) and enhance the adaptive immunity as the adjuvant against infections. To explore the underlying mechanism of immunomodulatory activities of AEAR, DCs were produced from bone-marrow cells of mice and the effects of AEAR on cell viability were assessed by the Cell Counting Kit 8 (CCK8) method and annexin V/propidium iodide staining assays. Then, the effects of AEAR on the morphology, maturation, and function of DCs were detected using a microscope, flow cytometry-based surface receptor characterization, and endocytosis assays. The secretion levels of cytokines were then analyzed with enzyme-linked immunosorbent assay (ELISA). The activation state of DCs was evaluated by the mixed lymphocyte reaction (MLR). The activity of MAPKs and NF-κB pathways, which were involved in the regulation of AEAR on DCs, was further detected by Western blot. AEAR did not have a cytotoxic effect on DCs or mouse splenocytes. AEAR remarkably enhanced the phenotypic maturation of DCs and promoted the expression of costimulatory molecules and the secretion of cytokines in DCs. AEAR also significantly decreased the phagocytic ability of DCs and augmented the abilities of DCs to present antigens and stimulate allogeneic T-cell proliferation. Simultaneously, AEAR potently activated toll-like receptor (TLR)4-/TLR2-related MAPKs and induced the degradation of IκB and the translocation of NF-κB. In short, AEAR can profoundly enhance the immune-modulating activities of DCs via TLR4-/TLR2-mediated activation of MAPKs and NF-κB signaling pathways and is a promising candidate immunopotentiator for vaccines. |
| first_indexed | 2024-03-10T16:21:22Z |
| format | Article |
| id | doaj.art-3fe9c3f26ec446399fe6cf63a610c09f |
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| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T16:21:22Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-3fe9c3f26ec446399fe6cf63a610c09f2023-11-20T13:35:24ZengMDPI AGVaccines2076-393X2020-09-018352510.3390/vaccines8030525Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic CellsYu Yang0DanYang Wang1QuanXiao Li2Jiang He3Bin Wang4Jinyao Li5Ailian Zhang6Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaKey Laboratory of Uighur Medicine, Xinjiang Institute of Materia Medica, Xinjiang 830004, ChinaKey Lab of Medical Molecular Virology, School of Basic Medical Science, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China<i>Artemisia rupestris</i> L. has long been used as a traditional herbal medicine owing to its immunomodulatory activity. Aqueous extracts of <i>Artemisia rupestris</i> L. (AEAR) contain the main functional component and can activate the maturation of dendritic cells (DCs) and enhance the adaptive immunity as the adjuvant against infections. To explore the underlying mechanism of immunomodulatory activities of AEAR, DCs were produced from bone-marrow cells of mice and the effects of AEAR on cell viability were assessed by the Cell Counting Kit 8 (CCK8) method and annexin V/propidium iodide staining assays. Then, the effects of AEAR on the morphology, maturation, and function of DCs were detected using a microscope, flow cytometry-based surface receptor characterization, and endocytosis assays. The secretion levels of cytokines were then analyzed with enzyme-linked immunosorbent assay (ELISA). The activation state of DCs was evaluated by the mixed lymphocyte reaction (MLR). The activity of MAPKs and NF-κB pathways, which were involved in the regulation of AEAR on DCs, was further detected by Western blot. AEAR did not have a cytotoxic effect on DCs or mouse splenocytes. AEAR remarkably enhanced the phenotypic maturation of DCs and promoted the expression of costimulatory molecules and the secretion of cytokines in DCs. AEAR also significantly decreased the phagocytic ability of DCs and augmented the abilities of DCs to present antigens and stimulate allogeneic T-cell proliferation. Simultaneously, AEAR potently activated toll-like receptor (TLR)4-/TLR2-related MAPKs and induced the degradation of IκB and the translocation of NF-κB. In short, AEAR can profoundly enhance the immune-modulating activities of DCs via TLR4-/TLR2-mediated activation of MAPKs and NF-κB signaling pathways and is a promising candidate immunopotentiator for vaccines.https://www.mdpi.com/2076-393X/8/3/525water-extractable polysaccharides<i>Artemisia rupestris</i> L.dendritic cellstoll-like receptorsimmunomodulation |
| spellingShingle | Yu Yang DanYang Wang QuanXiao Li Jiang He Bin Wang Jinyao Li Ailian Zhang Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells Vaccines water-extractable polysaccharides <i>Artemisia rupestris</i> L. dendritic cells toll-like receptors immunomodulation |
| title | Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells |
| title_full | Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells |
| title_fullStr | Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells |
| title_full_unstemmed | Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells |
| title_short | Immune-Enhancing Activity of Aqueous Extracts from <i>Artemisia rupestris</i> L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells |
| title_sort | immune enhancing activity of aqueous extracts from i artemisia rupestris i l via mapk and nf kb pathways of tlr4 tlr2 downstream in dendritic cells |
| topic | water-extractable polysaccharides <i>Artemisia rupestris</i> L. dendritic cells toll-like receptors immunomodulation |
| url | https://www.mdpi.com/2076-393X/8/3/525 |
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