DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of theLeptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immun...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2015-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000800989&lng=en&tlng=en |
| _version_ | 1797756765075406848 |
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| author | Karine M Forster Daiane D Hartwig Thaís L Oliveira Kátia L Bacelo Rodrigo Schuch Marta G Amaral Odir A Dellagostin |
| author_facet | Karine M Forster Daiane D Hartwig Thaís L Oliveira Kátia L Bacelo Rodrigo Schuch Marta G Amaral Odir A Dellagostin |
| author_sort | Karine M Forster |
| collection | DOAJ |
| description | Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of theLeptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain ofLeptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development. |
| first_indexed | 2024-03-12T18:06:17Z |
| format | Article |
| id | doaj.art-3feee00e01b94447b7c03de22ba61571 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:06:17Z |
| publishDate | 2015-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-3feee00e01b94447b7c03de22ba615712023-08-02T09:28:25ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-12-01110898999510.1590/0074-02760150222S0074-02762015000800989DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosisKarine M ForsterDaiane D HartwigThaís L OliveiraKátia L BaceloRodrigo SchuchMarta G AmaralOdir A DellagostinLeptospirosis is a zoonotic disease caused by pathogenic spirochetes of theLeptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain ofLeptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000800989&lng=en&tlng=enleptospirosisLeptospiraLigBrepsubunit vaccineDNA vaccineprime-boost |
| spellingShingle | Karine M Forster Daiane D Hartwig Thaís L Oliveira Kátia L Bacelo Rodrigo Schuch Marta G Amaral Odir A Dellagostin DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis Memorias do Instituto Oswaldo Cruz leptospirosis Leptospira LigBrep subunit vaccine DNA vaccine prime-boost |
| title | DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis |
| title_full | DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis |
| title_fullStr | DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis |
| title_full_unstemmed | DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis |
| title_short | DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis |
| title_sort | dna prime protein boost based vaccination with a conserved region of leptospiral immunoglobulin like a and b proteins enhances protection against leptospirosis |
| topic | leptospirosis Leptospira LigBrep subunit vaccine DNA vaccine prime-boost |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000800989&lng=en&tlng=en |
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