Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus
Abstract The Gram‐negative pathogen Vibrio vulnificus produces several iron‐sequestration systems including a hemin uptake system in response to iron limitation as a means to acquire this essential element. Strains of this organism are capable of causing serious septicemia in humans and eels, where...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-10-01
|
| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.905 |
| _version_ | 1818266462553374720 |
|---|---|
| author | Shreya Datta Ryan J. Kenton |
| author_facet | Shreya Datta Ryan J. Kenton |
| author_sort | Shreya Datta |
| collection | DOAJ |
| description | Abstract The Gram‐negative pathogen Vibrio vulnificus produces several iron‐sequestration systems including a hemin uptake system in response to iron limitation as a means to acquire this essential element. Strains of this organism are capable of causing serious septicemia in humans and eels, where hemin is abundant and an advantageous source of iron. Vibrio vulnificus hemin uptake systems consist of HupA, a well studied outer membrane protein, and a recently identified HvtA protein receptor. In this study, we confirmed that the expression of the hvtA gene is iron‐regulated in a fur‐dependent manner. When analyzed for virulence in a hemin‐overloaded murine model system, the hupA gene was more important for establishing infection than the hvtA gene. Transcriptional profiling of these genes using strains of two different biotypes, biotype 1 (human pathogen) and biotype 2 (eel pathogen), showed that the expression of the two receptors was also regulated in response to temperature. The expression of hupA was highly induced in elevated temperatures in the human pathogenic strain when tested in iron‐depleted conditions. Conversely, hvtA expression was induced significantly in the eel pathogenic strain at a lower temperature, a condition where the hupA locus was relatively repressed. Our results indicate that although both hupA and hvtA are involved for optimal hemin uptake in V. vulnificus, their expression is dually regulated by the environmental cues of iron concentration and temperature. Together, these data suggest that the virulence genes hupA and hvtA are tightly regulated and strictly induced during iron limitation combined with the physiological temperature of the host organism. |
| first_indexed | 2024-12-12T20:07:05Z |
| format | Article |
| id | doaj.art-3feef34e49af4cf9a53fe1541367574f |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-12T20:07:05Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-3feef34e49af4cf9a53fe1541367574f2022-12-22T00:13:36ZengWileyMicrobiologyOpen2045-88272019-10-01810n/an/a10.1002/mbo3.905Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificusShreya Datta0Ryan J. Kenton1Acumed LLC USA Headquarters Hillsboro OR USADepartment of Biology University of Portland Portland OR USAAbstract The Gram‐negative pathogen Vibrio vulnificus produces several iron‐sequestration systems including a hemin uptake system in response to iron limitation as a means to acquire this essential element. Strains of this organism are capable of causing serious septicemia in humans and eels, where hemin is abundant and an advantageous source of iron. Vibrio vulnificus hemin uptake systems consist of HupA, a well studied outer membrane protein, and a recently identified HvtA protein receptor. In this study, we confirmed that the expression of the hvtA gene is iron‐regulated in a fur‐dependent manner. When analyzed for virulence in a hemin‐overloaded murine model system, the hupA gene was more important for establishing infection than the hvtA gene. Transcriptional profiling of these genes using strains of two different biotypes, biotype 1 (human pathogen) and biotype 2 (eel pathogen), showed that the expression of the two receptors was also regulated in response to temperature. The expression of hupA was highly induced in elevated temperatures in the human pathogenic strain when tested in iron‐depleted conditions. Conversely, hvtA expression was induced significantly in the eel pathogenic strain at a lower temperature, a condition where the hupA locus was relatively repressed. Our results indicate that although both hupA and hvtA are involved for optimal hemin uptake in V. vulnificus, their expression is dually regulated by the environmental cues of iron concentration and temperature. Together, these data suggest that the virulence genes hupA and hvtA are tightly regulated and strictly induced during iron limitation combined with the physiological temperature of the host organism.https://doi.org/10.1002/mbo3.905HeminHupAHvtAIronVibrio vulnificus |
| spellingShingle | Shreya Datta Ryan J. Kenton Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus MicrobiologyOpen Hemin HupA HvtA Iron Vibrio vulnificus |
| title | Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus |
| title_full | Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus |
| title_fullStr | Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus |
| title_full_unstemmed | Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus |
| title_short | Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus |
| title_sort | characterization of temperature dependent hemin uptake receptors hupa and hvta in vibrio vulnificus |
| topic | Hemin HupA HvtA Iron Vibrio vulnificus |
| url | https://doi.org/10.1002/mbo3.905 |
| work_keys_str_mv | AT shreyadatta characterizationoftemperaturedependentheminuptakereceptorshupaandhvtainvibriovulnificus AT ryanjkenton characterizationoftemperaturedependentheminuptakereceptorshupaandhvtainvibriovulnificus |