Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil
Abstract Listeria monocytogenes is an opportunistic pathogen that causes listeriosis, a foodborne disease with low incidence but with high mortality rate in humans. This microorganism has been recovered from several dairy products, especially those produced with raw milk. The objective of this work...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academia Brasileira de Ciências
2021-04-01
|
| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/aabc/v93n3/0001-3765-aabc-93-03-e20190200.pdf |
| _version_ | 1798026424780587008 |
|---|---|
| author | LEANDRO PARUSSOLO RICARDO ANTÔNIO P. SFACIOTTE KARINE ANDREZZA DALMINA FERNANDA DANIELLE MELO UBIRAJARA M. DA COSTA SANDRA MARIA FERRAZ |
| author_facet | LEANDRO PARUSSOLO RICARDO ANTÔNIO P. SFACIOTTE KARINE ANDREZZA DALMINA FERNANDA DANIELLE MELO UBIRAJARA M. DA COSTA SANDRA MARIA FERRAZ |
| author_sort | LEANDRO PARUSSOLO |
| collection | DOAJ |
| description | Abstract Listeria monocytogenes is an opportunistic pathogen that causes listeriosis, a foodborne disease with low incidence but with high mortality rate in humans. This microorganism has been recovered from several dairy products, especially those produced with raw milk. The objective of this work was to investigate the presence of virulence genes, and also to define the antimicrobial susceptibility profile of L. monocytogenes isolates recovered from serrano artisanal cheese produced in Southern region of Brazil. Nine strains of L. monocytogenes (serotypes 1/2b and 4b) were evaluated through PCR to detect the presence of the virulence genes hly, inlA, inlC, inlJ, actA, plcB and iap, while antimicrobial susceptibility profile was determined via disk diffusion method. All strains exhibited the presence of the genes hly and plcB, whereas the other genes (iap, actA, inlA, inlC and inlJ) were only detected in eight strains. We verified that all strains were resistant to at least one antimicrobial agent and three of them showed multidrug resistance. These findings demonstrated the serrano artisanal cheese offers risks to consumers’ health and point to a need of adaptations and monitoring of manufacturing process of this food, in order to prevent the dissemination of L. monocytogenes. |
| first_indexed | 2024-04-11T18:36:16Z |
| format | Article |
| id | doaj.art-3fef3f60b8574aed8a21a0cc622284db |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T18:36:16Z |
| publishDate | 2021-04-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-3fef3f60b8574aed8a21a0cc622284db2022-12-22T04:09:16ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-04-0193310.1590/0001-3765202120190200Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of BrazilLEANDRO PARUSSOLOhttps://orcid.org/0000-0003-0150-959XRICARDO ANTÔNIO P. SFACIOTTEhttps://orcid.org/0000-0002-0198-5695KARINE ANDREZZA DALMINAhttps://orcid.org/0000-0002-7652-4025FERNANDA DANIELLE MELOhttps://orcid.org/0000-0002-3659-7940UBIRAJARA M. DA COSTAhttps://orcid.org/0000-0002-7571-3430SANDRA MARIA FERRAZhttps://orcid.org/0000-0002-1826-8713Abstract Listeria monocytogenes is an opportunistic pathogen that causes listeriosis, a foodborne disease with low incidence but with high mortality rate in humans. This microorganism has been recovered from several dairy products, especially those produced with raw milk. The objective of this work was to investigate the presence of virulence genes, and also to define the antimicrobial susceptibility profile of L. monocytogenes isolates recovered from serrano artisanal cheese produced in Southern region of Brazil. Nine strains of L. monocytogenes (serotypes 1/2b and 4b) were evaluated through PCR to detect the presence of the virulence genes hly, inlA, inlC, inlJ, actA, plcB and iap, while antimicrobial susceptibility profile was determined via disk diffusion method. All strains exhibited the presence of the genes hly and plcB, whereas the other genes (iap, actA, inlA, inlC and inlJ) were only detected in eight strains. We verified that all strains were resistant to at least one antimicrobial agent and three of them showed multidrug resistance. These findings demonstrated the serrano artisanal cheese offers risks to consumers’ health and point to a need of adaptations and monitoring of manufacturing process of this food, in order to prevent the dissemination of L. monocytogenes.http://www.scielo.br/pdf/aabc/v93n3/0001-3765-aabc-93-03-e20190200.pdfdairy productsmultidrug resistanceraw milkvirulence genes |
| spellingShingle | LEANDRO PARUSSOLO RICARDO ANTÔNIO P. SFACIOTTE KARINE ANDREZZA DALMINA FERNANDA DANIELLE MELO UBIRAJARA M. DA COSTA SANDRA MARIA FERRAZ Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil Anais da Academia Brasileira de Ciências dairy products multidrug resistance raw milk virulence genes |
| title | Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil |
| title_full | Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil |
| title_fullStr | Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil |
| title_full_unstemmed | Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil |
| title_short | Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil |
| title_sort | detection of virulence genes and antimicrobial susceptibility profile of listeria monocytogenes isolates recovered from artisanal cheese produced in the southern region of brazil |
| topic | dairy products multidrug resistance raw milk virulence genes |
| url | http://www.scielo.br/pdf/aabc/v93n3/0001-3765-aabc-93-03-e20190200.pdf |
| work_keys_str_mv | AT leandroparussolo detectionofvirulencegenesandantimicrobialsusceptibilityprofileoflisteriamonocytogenesisolatesrecoveredfromartisanalcheeseproducedinthesouthernregionofbrazil AT ricardoantoniopsfaciotte detectionofvirulencegenesandantimicrobialsusceptibilityprofileoflisteriamonocytogenesisolatesrecoveredfromartisanalcheeseproducedinthesouthernregionofbrazil AT karineandrezzadalmina detectionofvirulencegenesandantimicrobialsusceptibilityprofileoflisteriamonocytogenesisolatesrecoveredfromartisanalcheeseproducedinthesouthernregionofbrazil AT fernandadaniellemelo detectionofvirulencegenesandantimicrobialsusceptibilityprofileoflisteriamonocytogenesisolatesrecoveredfromartisanalcheeseproducedinthesouthernregionofbrazil AT ubirajaramdacosta detectionofvirulencegenesandantimicrobialsusceptibilityprofileoflisteriamonocytogenesisolatesrecoveredfromartisanalcheeseproducedinthesouthernregionofbrazil AT sandramariaferraz detectionofvirulencegenesandantimicrobialsusceptibilityprofileoflisteriamonocytogenesisolatesrecoveredfromartisanalcheeseproducedinthesouthernregionofbrazil |