Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression
The ATP1A2 coding α2 subunit of Na,K‐ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2tmCKwk/+) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and head...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-06-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12848 |
| _version_ | 1819230874733903872 |
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| author | Hiroki Sugimoto Masaaki Sato Junichi Nakai Kiyoshi Kawakami |
| author_facet | Hiroki Sugimoto Masaaki Sato Junichi Nakai Kiyoshi Kawakami |
| author_sort | Hiroki Sugimoto |
| collection | DOAJ |
| description | The ATP1A2 coding α2 subunit of Na,K‐ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2tmCKwk/+) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and headache. However, astrocytic properties during CSD have not been examined in FHM2 model mice. Using Atp1a2tmCKwk/+ crossed with transgenic mice expressing G‐CaMP7 in cortical neurons and astrocytes (Atp1a2+/−), we analyzed the changes in Ca2+ concentrations during CSD. The propagation speed of Ca2+ waves and the percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− were higher than those in wild‐type mice. Increased percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− may contribute to FHM2 pathophysiology. |
| first_indexed | 2024-12-23T11:36:00Z |
| format | Article |
| id | doaj.art-3ffa051a7ab94110b181a16f7dd72830 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-23T11:36:00Z |
| publishDate | 2020-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-3ffa051a7ab94110b181a16f7dd728302022-12-21T17:48:38ZengWileyFEBS Open Bio2211-54632020-06-011061031104310.1002/2211-5463.12848Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depressionHiroki Sugimoto0Masaaki Sato1Junichi Nakai2Kiyoshi Kawakami3Division of Biology Center for Molecular Medicine Jichi Medical University Tochigi JapanGraduate School of Science and Engineering Saitama University JapanGraduate School of Science and Engineering Saitama University JapanDivision of Biology Center for Molecular Medicine Jichi Medical University Tochigi JapanThe ATP1A2 coding α2 subunit of Na,K‐ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2tmCKwk/+) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and headache. However, astrocytic properties during CSD have not been examined in FHM2 model mice. Using Atp1a2tmCKwk/+ crossed with transgenic mice expressing G‐CaMP7 in cortical neurons and astrocytes (Atp1a2+/−), we analyzed the changes in Ca2+ concentrations during CSD. The propagation speed of Ca2+ waves and the percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− were higher than those in wild‐type mice. Increased percentages of astrocytes with elevated Ca2+ concentrations in Atp1a2+/− may contribute to FHM2 pathophysiology.https://doi.org/10.1002/2211-5463.12848astrocyteAtp1a2calcium imagingCSDFHM2G‐CaMP7 |
| spellingShingle | Hiroki Sugimoto Masaaki Sato Junichi Nakai Kiyoshi Kawakami Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression FEBS Open Bio astrocyte Atp1a2 calcium imaging CSD FHM2 G‐CaMP7 |
| title | Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression |
| title_full | Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression |
| title_fullStr | Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression |
| title_full_unstemmed | Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression |
| title_short | Astrocytes in Atp1a2‐deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression |
| title_sort | astrocytes in atp1a2 deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression |
| topic | astrocyte Atp1a2 calcium imaging CSD FHM2 G‐CaMP7 |
| url | https://doi.org/10.1002/2211-5463.12848 |
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