The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes
Objective We investigated how pituitary adenylate cyclase-activating polypeptide (PACAP) affects embryonic development during pre-in vitro maturation (pre-IVM) using porcine oocytes isolated from small follicles. Methods We divided the follicles into the experimental groups by size (SF, small follic...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2019-12-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-19-0162.pdf |
| _version_ | 1819172923263418368 |
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| author | Kyu-Mi Park Kyu-Jun Kim Minghui Jin Yongquan Han Kyoung-Ha So Sang-Hwan Hyun |
| author_facet | Kyu-Mi Park Kyu-Jun Kim Minghui Jin Yongquan Han Kyoung-Ha So Sang-Hwan Hyun |
| author_sort | Kyu-Mi Park |
| collection | DOAJ |
| description | Objective We investigated how pituitary adenylate cyclase-activating polypeptide (PACAP) affects embryonic development during pre-in vitro maturation (pre-IVM) using porcine oocytes isolated from small follicles. Methods We divided the follicles into the experimental groups by size (SF, small follicles; MF, medium follicles) and treated with and without PACAP and cultured for 18 hours (Pre-SF[−]PACAP; without PACAP, Pre-SF[+]PACAP; with PACAP) before undergoing IVM. The gene expression related to extracellular matrix formation (amphiregulin, epiregulin, and hyaluronan synthase 2 [HAS2]) and apoptosis (Bcl-2-associated X [BAX], B-cell lymphoma 2, and cysteine-aspartic acid protease 3) was investigated after maturation. The impact on developmental competence was assessed by the cleavage and blastocyst rate and total cell number of blastocysts in embryos generated from parthenogenesis (PA) and in vitro fertilization (IVF). Results Cleavage rates in the Pre-SF(+)PACAP after PA were significantly higher than SF and Pre-SF(−)PACAP (p<0.05). The cleavage rates between MF and Pre- SF(+)PACAP groups yielded no notable differences after IVF. Pre-SF(+)PACAP displayed the higher rate of blastocyst formation and greater total cell number than SF and Pre-SF(−)PACAP (p<0.05). Cumulus cells showed significant upregulation of HAS2 mRNA in the Pre-SF(+)PACAP compared to the SF (p<0.05). In comparison to other groups, the Pre-SF(+)PACAP group displayed a downregulation in mRNA expression of BAX in matured oocytes (p<0.05). Conclusion The PACAP treatment during pre-IVM improved the developmental potential of porcine oocytes derived from SF by regulating cumulus expansion and apoptosis of oocytes. |
| first_indexed | 2024-12-22T20:14:53Z |
| format | Article |
| id | doaj.art-3ffd10fea29d47bfaccd81b073e11909 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-22T20:14:53Z |
| publishDate | 2019-12-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-3ffd10fea29d47bfaccd81b073e119092022-12-21T18:13:59ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172019-12-0132121844185310.5713/ajas.19.016224330The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytesKyu-Mi Park0Kyu-Jun Kim1Minghui Jin2Yongquan Han3Kyoung-Ha So4Sang-Hwan Hyun5 Institute for Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, Korea Institute for Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, Korea Institute for Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, Korea Institute for Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, Korea Institute for Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, Korea Institute for Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, KoreaObjective We investigated how pituitary adenylate cyclase-activating polypeptide (PACAP) affects embryonic development during pre-in vitro maturation (pre-IVM) using porcine oocytes isolated from small follicles. Methods We divided the follicles into the experimental groups by size (SF, small follicles; MF, medium follicles) and treated with and without PACAP and cultured for 18 hours (Pre-SF[−]PACAP; without PACAP, Pre-SF[+]PACAP; with PACAP) before undergoing IVM. The gene expression related to extracellular matrix formation (amphiregulin, epiregulin, and hyaluronan synthase 2 [HAS2]) and apoptosis (Bcl-2-associated X [BAX], B-cell lymphoma 2, and cysteine-aspartic acid protease 3) was investigated after maturation. The impact on developmental competence was assessed by the cleavage and blastocyst rate and total cell number of blastocysts in embryos generated from parthenogenesis (PA) and in vitro fertilization (IVF). Results Cleavage rates in the Pre-SF(+)PACAP after PA were significantly higher than SF and Pre-SF(−)PACAP (p<0.05). The cleavage rates between MF and Pre- SF(+)PACAP groups yielded no notable differences after IVF. Pre-SF(+)PACAP displayed the higher rate of blastocyst formation and greater total cell number than SF and Pre-SF(−)PACAP (p<0.05). Cumulus cells showed significant upregulation of HAS2 mRNA in the Pre-SF(+)PACAP compared to the SF (p<0.05). In comparison to other groups, the Pre-SF(+)PACAP group displayed a downregulation in mRNA expression of BAX in matured oocytes (p<0.05). Conclusion The PACAP treatment during pre-IVM improved the developmental potential of porcine oocytes derived from SF by regulating cumulus expansion and apoptosis of oocytes.http://www.ajas.info/upload/pdf/ajas-19-0162.pdfoocyte maturationporcinefolliclesembryo developmentapoptosis |
| spellingShingle | Kyu-Mi Park Kyu-Jun Kim Minghui Jin Yongquan Han Kyoung-Ha So Sang-Hwan Hyun The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes Asian-Australasian Journal of Animal Sciences oocyte maturation porcine follicles embryo development apoptosis |
| title | The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes |
| title_full | The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes |
| title_fullStr | The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes |
| title_full_unstemmed | The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes |
| title_short | The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes |
| title_sort | use of pituitary adenylate cyclase activating polypeptide in the pre maturation system improves in vitro developmental competence from small follicles of porcine oocytes |
| topic | oocyte maturation porcine follicles embryo development apoptosis |
| url | http://www.ajas.info/upload/pdf/ajas-19-0162.pdf |
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