d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study
Abstract Objective Treatment with a combination of d-β-hydroxybutyrate (BHB) and melatonin (M) improves survival in hemorrhagic shock models. Our objective was to find the most effective melatonin concentration in combination with 4 molar BHB (4 M BHB). Survival and markers of organ injury were anal...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-017-2975-0 |
| _version_ | 1818476481330806784 |
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| author | Andrea Wolf Kristine E. Mulier Sydne L. Muratore Gregory J. Beilman |
| author_facet | Andrea Wolf Kristine E. Mulier Sydne L. Muratore Gregory J. Beilman |
| author_sort | Andrea Wolf |
| collection | DOAJ |
| description | Abstract Objective Treatment with a combination of d-β-hydroxybutyrate (BHB) and melatonin (M) improves survival in hemorrhagic shock models. Our objective was to find the most effective melatonin concentration in combination with 4 molar BHB (4 M BHB). Survival and markers of organ injury were analyzed in pigs exposed to pulmonary contusion, liver crush injury, and hemorrhagic shock and treated with lactated Ringer’s solution; 4 M BHB/43 mM M; 4 M BHB/20 mM M; 4 M BHB/10 mM M; 4 M BHB/4.3 mM M; or 4 M BHB/0.43 mM M. This work is an extension of a previously published research study. Results Survival was highest in pigs receiving 4 M BHB/43 mM M (13/14), followed by lactated Ringer’s solution (11/16) and BHB/M with decreased melatonin concentrations (4 M BHB/20 mM M 3/6, 4 M BHB/10 mM M 2/6, 4 M BHB/4.3 mM M 3/6, 4 M BHB/0.43 mM M 1/6, p = 0.011). High mortality was associated with increases in serum lactate, higher liver and muscle injury markers and decreases in PaO2:FiO2 ratios. Our study indicates that treatment with 4 M BHB and melatonin concentrations below 43 mM lack the survival benefit observed from 4 M BHB/43 mM melatonin in pigs experiencing hemorrhagic shock and polytrauma. |
| first_indexed | 2024-12-10T09:26:17Z |
| format | Article |
| id | doaj.art-3ffd1dde81a04ef3a0b7f4276764b709 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-10T09:26:17Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-3ffd1dde81a04ef3a0b7f4276764b7092022-12-22T01:54:30ZengBMCBMC Research Notes1756-05002017-11-011011710.1186/s13104-017-2975-0d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging studyAndrea Wolf0Kristine E. Mulier1Sydne L. Muratore2Gregory J. Beilman3Department of Surgery, University of MinnesotaDepartment of Surgery, University of MinnesotaDepartment of Surgery, University of MinnesotaDepartment of Surgery, University of MinnesotaAbstract Objective Treatment with a combination of d-β-hydroxybutyrate (BHB) and melatonin (M) improves survival in hemorrhagic shock models. Our objective was to find the most effective melatonin concentration in combination with 4 molar BHB (4 M BHB). Survival and markers of organ injury were analyzed in pigs exposed to pulmonary contusion, liver crush injury, and hemorrhagic shock and treated with lactated Ringer’s solution; 4 M BHB/43 mM M; 4 M BHB/20 mM M; 4 M BHB/10 mM M; 4 M BHB/4.3 mM M; or 4 M BHB/0.43 mM M. This work is an extension of a previously published research study. Results Survival was highest in pigs receiving 4 M BHB/43 mM M (13/14), followed by lactated Ringer’s solution (11/16) and BHB/M with decreased melatonin concentrations (4 M BHB/20 mM M 3/6, 4 M BHB/10 mM M 2/6, 4 M BHB/4.3 mM M 3/6, 4 M BHB/0.43 mM M 1/6, p = 0.011). High mortality was associated with increases in serum lactate, higher liver and muscle injury markers and decreases in PaO2:FiO2 ratios. Our study indicates that treatment with 4 M BHB and melatonin concentrations below 43 mM lack the survival benefit observed from 4 M BHB/43 mM melatonin in pigs experiencing hemorrhagic shock and polytrauma.http://link.springer.com/article/10.1186/s13104-017-2975-0Blood lossResuscitationMelatoninKetone bodiesAntioxidantHibernation |
| spellingShingle | Andrea Wolf Kristine E. Mulier Sydne L. Muratore Gregory J. Beilman d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study BMC Research Notes Blood loss Resuscitation Melatonin Ketone bodies Antioxidant Hibernation |
| title | d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study |
| title_full | d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study |
| title_fullStr | d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study |
| title_full_unstemmed | d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study |
| title_short | d-β-Hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury: a melatonin dose-ranging study |
| title_sort | d β hydroxybutyrate and melatonin for treatment of porcine hemorrhagic shock and injury a melatonin dose ranging study |
| topic | Blood loss Resuscitation Melatonin Ketone bodies Antioxidant Hibernation |
| url | http://link.springer.com/article/10.1186/s13104-017-2975-0 |
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